Hints For Successful Use Of The Binary Pump - Agilent Technologies 1260 Infinity II User Manual

Hints for Successful Use of the Binary Pump

• Place solvent cabinet with the solvent bottles always on top (or at a higher
• Flush the degasser with at least 5 mL per channel before operating the
• Prevent blocking of solvent inlet filters (never use the pump without solvent
• Check purge valve frit and column frit in regular time intervals. A blocked
• Whenever possible use a minimum flow rate of 5 μL/min per solvent
• Whenever exchanging the pump seals, the purge valve frit should be
• When using buffer solutions, flush the system with water before switching
• Check the pump pistons for scratches, grooves and dents when changing
• After changing the piston seals, apply the seal wear-in procedure (see
• Place the aqueous solvent on channel A and the organic solvent on
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual
level) of the pump.
pump, especially when the pumping system had been turned off for a
certain length of time (for example, overnight) and volatile solvent mixtures
are used in the channels.
inlet filters). Growth of algae should be avoided (see
Solvent Filters" on page 55).
purge valve frit can be identified by black, yellow or greenish layers on its
surface or by a pressure greater than 10 bar in low delay volume
configuration and 20 bar in standard configuration when pumping distilled
water at a rate of 5 mL/min with an open purge valve.
channel to avoid crossflow of solvent into the unused pump channel.
exchanged, too.
it off. The seal wash option should be used when installed, especially when
buffer solutions with concentrations of 0.1 M or higher are being pumped
for long periods of time.
the piston seals. Damaged pistons cause micro leaks and will decrease the
lifetime of the seals.
Wear-in Procedure"
channel B. The default compressibility settings are set accordingly.

Hints for Successful Use of the Binary Pump

on page 136).
Using the Pump
"Prevent Blocking of
"Seal
3
41