Prevent Blocking Of Solvent Filters; Checking The Solvent Filters - Agilent Technologies 1260 Infinity II User Manual

Prevent Blocking of Solvent Filters

Contaminated solvents or algae growth in the solvent bottle will reduce the
lifetime of the solvent filter and will influence the performance of the module.
This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7).
The following suggestions will prolong lifetime of the solvent filter and will
maintain the performance of the module.
• Use a sterile, if possible amber, solvent bottle to slow down algae growth.
• Filter solvents through filters or membranes that remove algae.
• Exchange solvents every two days or refilter.
• If the application permits add 0.0001 – 0.001 M sodium azide to the solvent.
• Place a layer of argon on top of your solvent.
• Avoid exposure of the solvent bottle to direct sunlight.
Never use the system without solvent filter installed.
N O T E

Checking the Solvent Filters

The solvent filters are located on the low-pressure side of the binary pump. A
blocked filter therefore does not necessarily affect the high pressure readings
of the pump. The pressure readings cannot be used to check whether the
filters are blocked or not. If the solvent cabinet is placed on top of the binary
pump, the filter condition can be checked in the following way:
Remove the solvent inlet tube from the inlet port of the solvent selection valve
or the degasser. If the filter is in good condition, the solvent will freely drip out
of the solvent tube (due to hydrostatic pressure). If the solvent filter is partly
blocked only very little solvent will drip out of the solvent tube.
When opening capillary or tube fittings, solvents may leak out.
WA R N I N G
The handling of toxic and hazardous solvents and reagents can carry health risks.
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InfinityLab LC Series 1260 Infinity II Binary Pump User Manual
Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
Using the Pump

Prevent Blocking of Solvent Filters

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