Power Up / Shut-Down The Pump; Prepare The Pump - Agilent Technologies 1290 Infinity User Manual

4
Using the Pump
Best Practices

Power up / Shut-down the pump

Power up the pump
• Use new or different mobile phase (as required).
• Purge each channel with 2.5 – 3 mL/min for 5 min. Open the manual purge
• Equilibrate your system with composition of your application for 15 min.
Long-term shut-down of the system
• Flush system with water to remove buffer.
• Remove all samples from the sampler and store according to good
• Use recommended solvents to store the system.
• Power off the system.

Prepare the pump

Purge
Use the Purge function to:
• fill the pump,
• exchange a solvent,
• remove air bubbles in tubes and pump heads.
Condition
Use the Conditioning function:
• daily when starting the pump,
• to minimize pressure ripple by dissolving air bubbles in pump heads.
Condition your complete system with solvents and composition of your application (for
N O T E
example 50 %/50 % A/B at 0.5 mL/min).
54
valve or use the purge command.
Use conditioning for 1290 Infinity II Pumps and G7104C.
laboratory practice.
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