Setup Of Basic Pump Parameters - Agilent Technologies 1260 Infinity II User Manual

3
Using the Pump
Setting up the Pump with the Instrument Control Interface

Setup of Basic Pump Parameters

The most important parameters of the pump are listed in
Table 3 Basic pump parameters
Parameter Limits
• Flow 0.001 – 5 mL/min
• Stop Time 0.01 min - no limit
• Post Time off - 99999 min
• Pressure Max: 0 – 600 bar
Limits Min: 0 – 600 bar
• Solvent A 0 – 100 %
• Solvent B off - 100 %
• Solvent H O, ACN, MeOH, IPA
2
type
• Solvent
Comment
• Timetable max. number of lines
depends on free
space in pump
memory
• Display
44
Description
Total flow rate of the pump. See
page 63 for pump hardware modifications to achieve lowest delay volume.
The stop time of the pump usually controls the run time of the whole LC system.
Use no limit to stop the run manually (useful for method development).
Time between the end of a run and the start of the next. Used for column
equilibration after a gradient.
Max must be bigger than Min! Set max pressure to the maximum operating
pressure of your column. A min pressure setting of e.g. 10 bar will turn off your
pump automatically when running out of solvent. A smarter way, however, is to
use the bottle fillings function (see
Although channel A can be set to 0 %, it cannot be turned off. This channel
should be used for the aqueous phase (water).
The percentage of channel B is automatically complemented by channel A to
give 100 %.
Select the solvent you are using in the respective solvent channel from the
drop-down list. In case your solvent is not listed, perform a solvent
compressibility calibration (see
Calibration" on page 104).
For details on solvent compressibility see
Calibration" on page 103.
Free text field for a description of the solvent. This description will show up in
method printouts, etc.
Use the timetable to build solvent gradients, flow gradients, or combinations of
both. Gradients are always linear. Use multiple timetable entries to mimic
exponential or parabolic gradients.
There are three ways to display the timetable:
• in tabular form
• as flow/pressure graph
• as solvent percentage plot
Values can only be changed in tabular view.
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual
Table 3
"When to Remove Damper and Mixer"
"Bottle Filling" on page 47).
"Running the Solvent Compressibility
"Binary Pump Solvent Compressibility
on page 44.
on