GE ImageQuant LAS 4000 Original Instructions Manual page 74

6 Troubleshooting
6.6 Error messages and countermeasures
Error message
Please input a valid repeat limit
count.
CCD Cooling is not stable.
Dark frame correction will not be ac-
curate.
Do you want to continue?
Can not start the exposure because
the light source does not match the
method. Please exchange the light
source or select another method.
Cannot start the exposure. Flat frame
calibration has not been performed.
Please run flat frame calibration for
the selected settings.
Cannot start the exposure. Flat frame
calibration has not been performed
for digitization epi-illumination.
Please run flat frame calibration for
digitization epi-illumination.
Flat frame calibration data was not
found.
Cannot start the exposure. CCD cali-
bration has not been performed.
Please run CCD calibration for the
selected exposure time. Calibrate
now and continue?
Cannot start the exposure. CCD cali-
bration has not been performed.
Please run CCD calibration for the
selected exposure time.
Cannot start the exposure. Automatic
exposure requires CCD calibration.
Please run CCD calibration for the
exposure time range from 1/100 sec
to 2 hours.
74
Meaning
The number of exposures
is not approriate in Incre-
ment or Repetition mode
CCD calibration will not be
accurate since the temper-
ature is not stable (when
exposing an EtBr sample
before the CCD has
reached its target cooling
temperature)
The incorrect light source
is installed
There is no Flat Frame file
that satisfies the exposure
conditions.
CCD calibration is not
ready for exposure
The CCD is not calibrated
No CCD calibration file ex-
ists.
Getting Started with ImageQuant LAS 4000 28-9607-59 AB
Countermeasure
Change the number of expo-
sures or repeat limits to a suit-
able quantity
Click the OK button if you wish
to take an exposure without
correction
Check that the intended light
source(s) are correctly in place
and that correct method is
chosen
Create a correction file that
satisfies exposure conditions
using the Flat Frame Calibra-
tion function in the Option
menu
Click the OK button to start ex-
posure. CCD calibration for the
exposure conditions is initiated.
Create a correction file that
satisfies exposure conditions
using the CCD Calibration
function in the Option menu
Create a correction file using
the CCD Calibration function
in the Option menu. Perform
calibration for both over 2
hours and from 1/100 sec to 2
hours.