Operation; Installing The Rotor - Beckman Coulter JA-25.50 Manual

OPERATION

INSTALLING THE ROTOR

Tie-down Knob
Daisy
Knob
Lubricate
Threads
Dual-Locking Lid
➠
NOTE
Do not run an empty rotor. Place filled tubes in
at least two opposing cavities.
• Precool the rotor in the centrifuge or in a refrigerator before use—
especially before short runs—to ensure that the rotor reaches the
set temperature. A suggested precooling cycle is a minimum of
30 minutes at 2000 rpm at the required temperature.
• If fluid containment is required, use capped tubes or bottles. It is
strongly recommended that all containers carrying physiological
fluids be capped to prevent leakage.
• If you are using an Avanti J series centrifuge, select the JA-25.50
rotor.
• If you are using a microprocessor-controlled J2 series centrifuge,
enter rotor code 25.5. Refer to your centrifuge instruction manual
for additional information.
1. To prevent the rotor from sticking to the centrifuge drive hub,
apply a thin coat of Spinkote lubricant to the rotor drive pins and
to the lid knob threads.
2. Lightly coat the large and small lid O-rings with silicone vacuum
grease.
3. Dual-locking lid only. Place filled tubes into the rotor, then place
the lid on the rotor and turn the daisy knob to the right (clockwise)
until secure. Do not overtighten.
4. Carefully lower the rotor straight down onto the centrifuge drive
spindle hub. Do not drop the rotor onto the spindle hub.
5. Slowly turn the rotor around the drive spindle to make sure that it
is properly seated.
JA-25.50 Rotor
7