Description; Preparation And Use - Beckman Coulter Avanti J-26S XP Series Instructions For Use Manual

JS-5.3 Swinging-Bucket Rotor

Description

Description
Beckman Coulter JS-5.3 rotors are manufactured in a facility that maintains certifications to both
ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges.
The JS-5.3 is a four-place swinging-bucket rotor used in Beckman Coulter Avanti JXN-26, J-26S XP
series, Avanti J-26 XP series, Avanti J-E, and discontinued J-20 XP series centrifuges. Each rotor
bucket can carry a 96-well kit for high-throughput processing (such as a DNA or RNA kit), or
standard microplates used in the serial dilution of small liquid volumes—up to six stacked (not to
exceed 83.2 mm/3.28 in.) 96-well polypropylene plates, two (stacked) deep-well plates, or one
square-well plate per bucket. An array of adapters carry a wide range of tube and bottle sizes
(from 1.5 to 500 mL).
The rotor yoke is made of aluminum and is black-anodized for corrosion protection. Blue-anodized
aluminum buckets can be run by placing them over pivot pins on the arms of the yoke; they
swing out to horizontal position during centrifugation. A tie-down knob secures the rotor to the
centrifuge drive hub.
Aeroseal covers, made of transparent polyphenysulfone (PPSU) are available. These covers have
*
been tested
the associated Beckman Coulter rotors and centrifuges when used and maintained as instructed. In
the event of labware leakage, the covers will contain liquids and broken labware, reducing the need
to clean the centrifuge chamber and allowing you to take appropriate precautions before removing
the covers.
Each cover requires a replaceable silicone rubber O-ring that seats around the outside edge of the
cover. An air-vent filter allows passage of air, but not of liquids or aerosols larger than 0.3 micron,
in and out of the bucket to minimize the effects of vacuum conditions inside the centrifuge during
operation. The covers are held in place by attached latches.
Refer to the Warranty at the back of this manual for warranty information.

Preparation and Use

Specific information about the JS-5.3 rotor is given here. Use the J Series Rotors and Tubes Manual
(JR-IM) along with this rotor manual for complete rotor and accessory operation.
NOTE
Although rotor components and accessories made by other manufacturers may fit in the JS-5.3 rotor,
their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers'
components or accessories in the JS-5.3 rotor may void the rotor warranty and should be prohibited by
your laboratory safety officer. Only the components and accessories listed in this publication should be
used in this rotor.
* Validation of microbiological containment was done at an independent third-party testing facility, Health Protection
Agency, Porton Down. Improper use or maintenance may affect seal integrity and thus containment.
1-2
to demonstrate containment of microbiological aerosols under normal conditions of
PN J-TB-089AG