Run Speeds - Beckman Coulter Avanti J-26S XP Series Instructions For Use Manual

JS-5.3 Swinging-Bucket Rotor
Preparation and Use
Table 2 Tube and Bottle Adapters for the JS-5.3 Rotor (Continued)
Adapter
a. Maximum RCF for adapters. Use manufacturers' recommendations for tube and bottle limits.
b. Observe limitations described on page 8 when using bucket covers with these adapters.
c. Maximum RCF for labware in this adapter is 40000 ´ g.
d. Requires a cushion (356983) to be placed at bottom of adapter.
e. Cannot be used with bucket cover.
f. Use this adapter inside the 250-mL adapter (392077) to run tubes at 6670 ´ g.

Run Speeds

The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces
between different rotors are made by comparing the rotors' relative centrifugal fields (RCF). When
rotational speed is adjusted so that identical samples are subjected to the same rcf in two different
rotors, the samples are subjected to the same force (see
If the weight of the load in a bucket exceeds 1040 grams, or if the solution density is more than
1.2 g/mL, reduce the maximum allowable run speed according to the following equation:
Do not select rotational speeds in excess of 5300 RPM.
NOTE
Polystyrene plates are subject to cracking at high speeds. Test polystyrene plates using water in place
of sample to determine required speed deration.
1-14
Part
Adapter Number
Color (Pkg/4)
e
blue
392078
356966
yellow
(each)
356964
yellow
(each)
reduced maximum speed = (5300 RPM)
Maximum
Tubes/
Tube/Bottle Bottles per
Size Adapter
500 mL conical 1
f
1
50 mL conical
f
4
15 mL conical
Table 3 for examples).
1040 grams
------------------------------------------------------ -
heaviest load in grams
RCF at Max
Speed
a
(RCF)
6870 ´ g
6670 ´ g
6670 ´ g
PN J-TB-089AG