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Proteins and solutions containing surfactants are known to "un-condition" the measurement pedestal surfaces so that the liquid column
does not form. "Re-condition the pedestal as follows:
•
Apply 5-10 ul of ~0.5M of HCl to the bottom pedestal, lower the upper pedestal into the solution and let it sit for approximately 2
to 3 minutes. Wipe both pedestals with a clean lab wipe and repeat this using 5-10 ul of deionized water to wash away any
residue.
•
Fold a clean dry lab wipe over several times to increase its thickness. Press the lab wipe firmly down on the lower pedestal and
"buff/rub" very aggressively at least 50 times - the lab wipe will rip during this procedure and will have to be refolded several
times throughout the procedure. The upper pedestal may also be buffed but care should be taken not to put too much force on
the upper arm.
•
Test the effectiveness of the re-conditioning by pipetting a 1-2ul sample of water onto the lower measurement pedestal and
making sure the water "beads up" on the pedestal surface
If the warning persists and the user visually confirms that the liquid column is forming, contact NanoDrop Technologies or your local
distributor.
Error: Code 8
This error code is most likely to occur if the Windows account that is currently logged into Windows does not have read and write
access to the folder c:\nanodrop data or one of its subfolders. See your PC administrator to make sure that all users of the NanoDrop
software operate with a Windows account that has the appropriate access.
Can't Find LabView RunTime Engine...
This error message likely means that one or more of the software components have been removed or corrupted. If this occurs, reinstall
the Labview Runtime engine by selecting Start
Report Format Loading Error
This error occurs when the user does not have "Write" access to one of the report format files located at c:\nanodrop data\custom report
formats or the file has been moved from this folder. This error is similar to Error Code 8 (see above). Contact your PC administrator to
give all users Read and Write access to this folder. Replace the files if they have been removed. If the file cannot be located, reinstall
the software.
Other Software Error Messages
Can't find file OOIDRV.INI...
This error occurs when trying to install the software without administrator privileges. Contact your system administrator to install the
software.
Source Error...
.
Programs
NanoDrop Utilities
15-4
Runtime Installer.
Section 15- Troubleshooting
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