Saturated Detector; Liquid Column Breakage - Thermo Scientific NanoDrop 1000 V3.3 User Manual

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With the sampling arm down, select OK to initialize the spectrometer and then select 'Intensity Check'. You should see a red
and black spectrum and a bias value greater than 65 as shown below. This indicates that the USB communication is normal,
the power supply is operational and the flashlamp is functioning.
If no spectra appear in the image, confirm that the power supply is firmly connected to the instrument and the plug is
connected to a working outlet. Next, confirm that the power supply is operating properly. To do this, connect the leads of a
volt-ohmmeter to the outlet of the supply. The voltage should be 12-20 Vdc, center positive.
If none of the troubleshooting steps above solve the problem, refer to the Technical Service section for getting help from your distributor
or NanoDrop Technologies.

Saturated Detector

This error message can occur when the software calculates too high integration times during initialization. This is most likely due to a
dried sample left on the measurement surface after the last use of the instrument. Cleaning both the top and bottom pedestals with de-
ionized water and exiting out of the software module to the main menu should alleviate the problem. It is not necessary to close the
software completely as each module is re-initialized when it is opened. If the error persists, contact NanoDrop Technologies or your
local distributor.

Liquid Column Breakage

This warning occurs when a possible problem with the column is detected. The software compares the long path and short path
absorbances and issues a warning to the user if the short path is not 20% of the long path absorbance, within a tolerance. The most
common explanation is that the column is not forming properly due to the pedestal being "unconditioned".
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Section 15- Troubleshooting