Table Of Contents - Thermo Scientific NanoDrop 1000 V3.3 User Manual

Table of Contents

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1.
Overview ................................................................................................. 1-1
Instrument Description.............................................................................. 1-1
Operation.................................................................................................. 1-1
Applications .............................................................................................. 1-1
Patents ..................................................................................................... 1-1
2.
Initial Set Up............................................................................................ 2-1
Computer Requirements .......................................................................... 2-1
Software Installation ................................................................................. 2-1
Software Upgrades................................................................................... 2-1
Registering Your Instrument ..................................................................... 2-2
3.
General Operation .................................................................................. 3-1
The Sample Retention System ................................................................. 3-1
Cleaning the Sample Retention System ................................................... 3-1
Software Architecture and Features ......................................................... 3-2
User Preferences...................................................................................... 3-3
Utilities and Diagnostics ........................................................................... 3-3
Account Management............................................................................... 3-3
Dye/Chromophore Editor .......................................................................... 3-5
4.
Common Module Functions .................................................................. 4-6
Module Startup ......................................................................................... 4-6
Escape Key (ESC).................................................................................... 4-6
Measure (F1) ............................................................................................ 4-6
Blank (F3) ................................................................................................. 4-6
Blanking Cycle.......................................................................................... 4-6
Re-blank (F2)............................................................................................ 4-7
Print Screen (F4) ...................................................................................... 4-7
Saving Current Screen as .JPG Image..................................................... 4-7
Start Report / Recording (F6) ................................................................... 4-7
Print Report (F5)....................................................................................... 4-7
Show Report (F7) ..................................................................................... 4-8
Sample ID................................................................................................. 4-8
Sample # .................................................................................................. 4-8
Exit ........................................................................................................... 4-8
Show Context Help................................................................................... 4-8
User's Manual........................................................................................... 4-8
5.
Nucleic Acids .......................................................................................... 5-1
Sample Volume Requirements ................................................................. 5-1
Measurement Concentration Range......................................................... 5-1
Spectrum Normalization ........................................................................... 5-2
Spectrum Overlay Control ........................................................................ 5-2
6.
MicroArray............................................................................................... 6-1
Fluorescent Dye Selection........................................................................ 6-1
Sample Volume Requirements ................................................................. 6-1
Measurement Concentration Range......................................................... 6-1
Baseline Calculation & Normalization....................................................... 6-2
7.
UV-VIS ..................................................................................................... 7-1
Sample Volume Requirements ................................................................. 7-1
Measurement Concentration Range......................................................... 7-1
Unique Screen Features........................................................................... 7-1
8.
Protein A280............................................................................................ 8-1
Sample Volume Requirements ................................................................. 8-1
Special Cleaning Requirements for Proteins ............................................ 8-1
Measurement Concentration Range......................................................... 8-1
Unique Screen Features........................................................................... 8-1
Spectrum Normalization ........................................................................... 8-2
Spectrum Overlay Control ........................................................................ 8-2
9.
Proteins & Labels ................................................................................... 9-1
Fluorescent Dye Selection........................................................................ 9-1
Sample Volume Requirements ................................................................. 9-1
Special Cleaning Requirements for Proteins ............................................ 9-1

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Nd-1000

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