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λ and A: current value of the user-selectable wavelength cursor and corresponding absorbance. The wavelength can be set by
dragging the cursor, using the up/down arrows or typing in the desired wavelength. Note: The user-selected wavelength and
absorbance are not utilized in any calculations.
A280 10-mm Path: 10 mm-equivalent absorbance at 280 nm for the protein sample measured
A260/280: ratio of sample absorbance at 260 and 280 nm
Show Report: formatted for 200 samples although the buffer size can be increased to accommodate thousands of samples.
Spectrum Normalization
The baseline is automatically set to the absorbance value of the sample at 340 nm, which should be very nearly zero absorbance. All
spectra are referenced off of this zero. All data are now normalized and archived in the same format.
Spectrum Overlay Control
The user can display more than one spectrum in the same display using this feature. The current sample plot will be displayed in bold
and previous plots will be distinguished by different colors. See example below:
The default option is set to clear the display for the next reading. The user may set the overlay control to clear after each sample plot
(default setting), after each new report, or accumulate plots until prompted to clear. The clear now setting will clear all current and
previous plots. When the overlay function is active, the software module will auto scale the y-axis based on the sample with the highest
absorbance at 280nm. Note: When the overlay function is active, the 'Blank' function doesn't clear the existing overlaid sample
IgG reference. Unknown (sample) protein concentrations are calculated using the mass extinction coefficient of
13.7 at 280 nm for a 1% (10 mg/ml) IgG solution.
Lysozyme reference. Unknown (sample) protein concentrations are calculated using the mass extinction
coefficient of 26.4 at 280 nm for a 1% (10 mg/ml) Lysozyme solution.
User-entered values for molar extinction coefficient (M
respective protein reference. Maximum value for e is 999 X 1000 and maximum value for M.W. is 9999 X 1000.
User-entered mass extinction coefficient (L gm
protein.
-1
-1
cm
) and molecular weight (MW) in kilo Daltons for their
-1
-1
cm
) for a 10 mg/ml (1%) solution of the respective reference
8-2
Section 8- Protein A280
spectra.
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