Thermo Scientific NanoDrop 1000 V3.3 User Manual page 4

Measurement Concentration Range......................................................... 9-1
Unique Screen Features........................................................................... 9-1
Baseline Type........................................................................................... 9-2
10. Protein BCA .......................................................................................... 10-1
Sample Volume Requirements ............................................................... 10-1
Special Cleaning Requirements for Proteins .......................................... 10-1
Measurement Concentration Range....................................................... 10-1
BCA Kits, Protocols, and Sample Preparation........................................ 10-1
Unique Screen Features......................................................................... 10-1
Making BCA Measurements ................................................................... 10-2
Standard Curve Features ....................................................................... 10-2
Exiting the BCA Module.......................................................................... 10-3
11. Protein Lowry........................................................................................ 11-1
Sample Volume Requirements ............................................................... 11-1
Special Cleaning Requirements for Proteins .......................................... 11-1
Measurement Concentration Range....................................................... 11-1
Modified Lowry Kits, Protocols, and Sample Preparation ....................... 11-1
Unique Screen Features......................................................................... 11-1
Making Lowry Measurements................................................................. 11-2
Standard Curve Features ....................................................................... 11-2
Exiting the Lowry Module ....................................................................... 11-3
12. Protein Bradford ................................................................................... 12-1
Sample Volume Requirement................................................................. 12-1
Special Cleaning Requirements for Proteins .......................................... 12-1
Measurement Concentration Range....................................................... 12-1
Bradford Kits, Protocols, and Sample Preparation ................................. 12-1
Unique Screen Features......................................................................... 12-1
Making Bradford Protein Measurements ................................................ 12-2
Standard Curve Features ....................................................................... 12-3
Exiting the Bradford Module ................................................................... 12-3
13. Cell Cultures ......................................................................................... 13-1
Sample Size Requirements .................................................................... 13-1
Cell Suspension Concentrations............................................................. 13-1
Sample Homogeneity ............................................................................. 13-1
Decontamination of Measurement Pedestals ......................................... 13-2
14. Archived Data and Data Viewer........................................................... 14-1
Archive File Creation .............................................................................. 14-1
Data Storage Hierarchy .......................................................................... 14-1
Data Viewer ............................................................................................ 14-2
Archive File Converter ............................................................................ 14-5
Opening Archived Data with Spreadsheet Programs ............................. 14-6
15. Troubleshooting ................................................................................... 15-1
Error USB2000 ....................................................................................... 15-1
Connection Error .................................................................................... 15-2
Signal Error............................................................................................. 15-2
Saturated Detector ................................................................................. 15-3
Liquid Column Breakage ........................................................................ 15-3
Other Software Error Messages ............................................................. 15-4
Sample Accuracy and Reproducibility .................................................... 15-5
260/280 Ratio ......................................................................................... 15-6
Unusual Spectrum .................................................................................. 15-6
Technical Service ................................................................................... 15-7
16. Maintenance and Warranty .................................................................. 16-1
Cleaning ................................................................................................. 16-1
Calibration .............................................................................................. 16-1
Warranty ................................................................................................. 16-1
17. Appendices ........................................................................................... 17-1
Instrument Specifications ....................................................................... 17-1
Blanking and Absorbance Calculations .................................................. 17-1
Concentration Calculation (Beer's Law) ................................................. 17-1
Solvent Compatibility .............................................................................. 17-2
Decontamination of Measurement & Optical Surfaces ........................... 17-2
Setting Up a Dymo 400 Label Writer Printer........................................... 17-2