Spectrum Normalization; Spectrum Overlay Control - Thermo Scientific NanoDrop 1000 V3.3 User Manual

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Section 5- Nucleic Acids
Show Report: formatted for 200 samples although the buffer size can be increased to accommodate thousands of samples.

Spectrum Normalization

The baseline is automatically set to the absorbance value of the sample at 340 nm, which should be very nearly zero absorbance. All
spectra are referenced off of this zero.

Spectrum Overlay Control

The user can display more than one spectrum in the same display using this feature. The current sample plot will be displayed in bold
and previous plots will be distinguished by different colors. See example below:
The default option is set to clear the display for the next reading. The user may set the overlay control to clear after each sample plot,
(default setting), after each new report, or accumulate plots until prompted to clear. The Clear Now setting will clear all current and
previous plots. When the overlay function is active, the software will auto scale the y-axis based on the sample with the highest
absorbance at 260 nm. Note: When the overlay function is active, the 'Blank' function does not clear the existing overlaid sample
spectra.
5-2

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