Objectives For Various Observation Modes - Olympus BX-URA2 Instruction Manual

Exclusively for Fluorescent Proteins
Excitation
Mirror Unit
Method
CFP U-MCFPHQ
GFP U-MGFPHQ
YFP U-MYFPHQ
RFP U-MRFPHQ
Mirror Unit Name Meaning
U - M C F P H Q
3

Objectives for Various Observation Modes

UIS2 Series
UPlanSApo
PlanApoN
UPlanFLN
¦ : Recommended combination.
¦* : Slightly inferior in U-excitation.
– – : Not usable, or applicable objective is not available.
¦** : A phase contrast (Ph) objective is necessary for phase contrast observation.
10
Dichroic Mirror
DM450HQ
DM485HQ
DM505HQ
DM565HQ
Mirror unit
Universal
Objective
4X
10X 2
20X
20X O
40X 2
60X W
60X O
100X O
60X O
4X
10X 2
20X
40X
40X O
60X
60X OΙ
100X O2
100X OΙ2
Excitation Filter
BP425-445HQ
BP460-480HQ
BP490-500HQ
BP535-555HQ
High Quality
CFP/GFP/YFP/RFP
Reflected light
Phase contrast
fluorescence
¦
¦
¦
¦
¦
¦
¦
¦
¦*
¦
¦
¦
¦
¦
¦
¦
¦
¦
Barrier Filter
BA460-510HQ
BA495-540HQ
BA515-560HQ
BA570-625HQ
Transmitted light DIC
difference
–
–
–
–
–
–
–
–
–
–
¦**
¦**
¦**
–
–
¦**
¦**
–
Fluorochromes
For ECFP
For EGFP
For EYFP
For RFP
¦
¦
¦
¦
¦
¦
¦
¦
¦
–
¦
¦
¦
¦
–
¦
¦
¦