Exciter Balancers U-Exbabg/Exbaub/Exbaug (For Exclusive Use With The Bx-Rfa) - Olympus BX-URA2 Instruction Manual

Reflected fluorescence system
Table of Contents

Advertisement

4

Exciter Balancers U-EXBABG/EXBAUB/EXBAUG (for exclusive use with the BX-RFA)

}When an image of fluorescence by multiple excitation of U/B/G is observed with dual- or triple-band fluorescence mirror
units, use the exciter balancer to select the balance between the excitation light intensities of the fluorochromes.
Installation Procedure (Fig. 9)
@
U-EXBAUG
U-EXBABG/EXBAUB
Operation
Observing a Double Stained Specimen
1. Set up normal reflected fluorescence observation.
2. Mount the fluorescence mirror units for double staining and engage them in the light path.
}Olympus standard products
Exciter Balancer
U-EXBABG
U-EXBAUB
U-EXBAUG
28
Fig. 9
Fluorescence Mirror Unit
Fluorescence mirror units
Fluorescence mirror units
for double staining
for triple staining
· U-DM-FI/TR2
· U-DM-FI/PI2
· U-DM-FI/TX2
· U-DM-DA/FI2
· U-DM-DA/TR2
· U-DM-DA/PI2
· U-DM-DA/TX2
Clamping screw
1. Stand the adjustment lever @ of the exciter balancer vertically and insert
it in one of the ND filter inlets with the same number as the slider on the
left side of the illuminator, or into the one which is located near the illumi-
nator rear panel.
· The insertion position is variable depending on the type of the exciter
balancer.
· With any type of exciter balancer, always insert so that the clamping
screw faces toward you.
2. Tighten the clamping screws using the Allen screwdriver.
# Due to its own characteristics, the G-exci-
# Lighting irregularities may be observed on
· U-DM-DA/FI/TR2
· U-DM-DA/FI/PI2
· U-DM-DA/FI/TX2
Adjustment lever
tation has a narrower intensity control range
than the U- and B-excitation. The intensity
control range is also variable depending on
the status of specimen and mirror units.
the upper and lower edges of the field due
to the rotation angles of filters and the vari-
ance in mirror units' characteristics. How-
ever, these lighting irregularities does not
affect the photographed area.

Advertisement

Table of Contents
loading

Table of Contents