Olympus BX-RLA2 Instructions Manual
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Olympus BX-RLA2 Instructions Manual

Olympus BX-RLA2 Instructions Manual

Reflected light brightfield/darkfield illuminator
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INSTRUCTIONS
BX-RLA2
REFLECTED LIGHT BRIGHTFIELD/
DARKFIELD ILLUMINATOR
This instruction manual is for the Olympus Reflected Light Brightfield/Darkfield Illuminator Model BX-RLA2.
The BX-RLA2 is capable of brightfield and darkfield observations under the reflected light when it is
installed on the BX51RF/BX51TRF system microscope frame together with the BXFM focusing unit. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this unit, we
recommend that you study this manual thoroughly before operating the microscope. Retain this instruc-
tion manual in an easily accessible place near the work desk for future reference.
A X 7 6 2 1

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Summary of Contents for Olympus BX-RLA2

  • Page 1 This instruction manual is for the Olympus Reflected Light Brightfield/Darkfield Illuminator Model BX-RLA2. The BX-RLA2 is capable of brightfield and darkfield observations under the reflected light when it is installed on the BX51RF/BX51TRF system microscope frame together with the BXFM focusing unit. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this unit, we recommend that you study this manual thoroughly before operating the microscope.

  • Page 3: Table Of Contents

    — Be sure to read this chapter for safe use of the equipment. — 1 SYSTEM DIAGRAM 2 NOMENCLATURE OF BX-RLA2 3 REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE 4 USING THE CONTROLS OF BX-RLA2 7-11 Selecting the Light Path Centering the Field Iris Diameter (FS)

  • Page 4: Important - Be Sure To Read This Chapter For Safe Use Of The Equipment

    IMPORTANT Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. 3.
  • Page 5 BX-RLA2 Caution If this unit is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

  • Page 6: System Diagram

    SYSTEM DIAGRAM Reflected Light Brightfield/Darkfield Illuminator Reflected Light Brightfield/Darkfield BX-RLA2 Reflected Light Illuminator Lamp Housing BX-RLA2 Module Focusing Unit BXFM-F Illuminator Holder Stage for BXFM-ILH Metallurgic Specimens or Stage Plate* Microscope frame BX51RF BX51TRF * Replace the standard stage with the stage for metallurgic specimens or the specimen...

  • Page 7: Nomenclature Of Bx-Rla2

    BX-RLA2 NOMENCLATURE OF BX-RLA2 Polarizer insertion slot (Page 14) FS centering screws (Page 7) Field iris diaphragm (FS) knob Analyzer insertion slot (Page 14) (Page 7) Filter insertion slot (Page 11) #Always insert filters from the left side! Mirror selector lever...

  • Page 8: Reflected Light Brightfield/Darkfield Observation Procedure

    REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE }The following flow shows the basic operating procedure for reflected light brightfield or darkfield observation. The operating procedures for polarized light and Nomarski DIC observations will be described separately in their descriptions. (The micro- scope frame used is the BX51TRF.) (Controls Used) (Page) (Controls Used)
  • Page 9 BX-RLA2 š Insertion from the left ™ Œ ‹ ‰ ² ³ † ƒ Š … ‡ }Make a photocopy of the observation procedure page and post it near your microscope.

  • Page 10: Using The Controls Of Bx-Rla2

    USING THE CONTROLS OF BX-RLA2 Selecting the Light Path (Fig. 1) Slide the mirror selector lever @ toward the indication of the mirror for the desired observation method. BF: Reflected light brightfield observation DF: Reflected light darkfield observation # Be sure to slide the mirror selector lever until it contacts the stopper position.
  • Page 11 BX-RLA2 6. Open the field iris so that its image is almost the same size as (i.e. sub- scribes) the field of view. Field iris diaphragm image Using the Field Iris Diaphragm {In reflected light brightfield observation The field iris diaphragm adjusts the illuminated area to obtain an image with high contrast.

  • Page 12: Centering The Aperture Iris Diaphragm (As)

    (Fig. 3) Centering the Aperture Iris Diaphragm (AS) ² 1. Slide the mirror selector lever @ to “BF”. 2. Engage the 10X objective by rotating the revolving nosepiece and bring the specimen in approximate focus. ³ 3. Remove the eyepiece, look into the eyepiece sleeve and pull the AS knob ²...

  • Page 13: Using The Nd Filter Knob

    BX-RLA2 Using the ND Filter Knob (Fig. 4) }The ND filter is interlocked with the brightfield (BF) light path selecting so it can be engaged or disengaged according to the mirror selector lever @. The ND filter makes it possible to reduce glare when darkfield (DF) is switched to brightfield (BF).

  • Page 14: Using The Filters

    Using the Filters (Fig. 5) }Engage the optimum filter sliders for the purpose of observation in the two filter insertion slots @. Be sure to engage from the left side. The first click position is the idle position and the second click engages the filter in the light path.

  • Page 15: Stage For Metallurgical Specimens

    BX-RLA2 STAGE FOR METALLURGICAL SPECIMENS }To improve the ease of operation, it is recommended to use a stage for exclusive use with metallurgical specimens or replace the stage plate with a specimen holder. Placing the Specimen With the U-MSSP or U-MSSP4 Stage Plate Place the specimen on the stage plate @.

  • Page 16: Using The Y-Axis Lock Lever

    [U-SIC4R2/SIC4L2 Stage Only] Observation with the U-MSSPG Glass Holder Plate (Fig. 8) ² }If the microscope is equipped with transmitted light illumination, transpar- ent specimens can also be observed. 1. Place the glass plate ² gently on the glass holder plate @. 2.

  • Page 17: Observation Methods

    BX-RLA2 OBSERVATION METHODS (Using BX-RLA2) 6-1 Reflected Light Brightfield Observation See “REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE” on page 5. 6-2 Reflected Light Nomarski DIC (Differential Interference Contrast) Observation # The performance of polarizer may deteriorate when it has been exposed to light for a long period (about continu- ous 2000 hours).
  • Page 18 Using the Joint Plate (Fig. 11) ƒ … When the U-AN360-3 analyzer ƒ and U-PO3 or U-POTP3 polarizer … are coupled by using the joint plate | provided with the polarizer and tighten- ing the clamping knobs on it, the analyzer and polarizer can be engaged or disengaged in the light path together.
  • Page 19 BX-RLA2 3. With the U-DICRH or U-DICRHC slider that does not have the slide lever, the applicable objectives are as follows. DIC Slider Applicable Objectives U-DICRH MPLFLN/MPLFLN-BD series UIS2 UMPlanFl/UMPlanFl-BD series MPlanFl-BD series MPlanApo20X, 100X U-DICRHC LMPLFLN/LMPLFLN-BD series UIS2 LMPlanFl/LMPlanFl-BD series...
  • Page 20 Observation Method (Fig. 12) 1. Place the specimen on the stage and adjust the focus by moving the stage up or down. 2. Adjust the field iris diaphragm so that its image circumscribes the field of view. 3. The contrast may sometimes be enhanced by closing the aperture iris diaphragm slightly.
  • Page 21 BX-RLA2 U-DICRH 1. Adjust the background contrast by turning the slider movement knob | on the DIC slider as described below. (Fig. 12) 2. When the slider movement knob on the U-DICRH DIC slider is turned, the interference color in the background varies from -100 to 100 nm. Set the retardation which can provide best contrast.

  • Page 22: 6-3 Reflected Light Simplified Polarized Light Observation

    Switching to Brightfield/Darkfield Observation (Fig. 12) 1. Loosen the mounting knob @ on the front of the DIC revolving nosepiece, gently pull out the DIC slider ² until it clicks, then clamp by tightening the mounting knob. 2. Slide the analyzer (U-AN360-3) and polarizer to disengage them from the light path.

  • Page 23: Specifications

    Item Specification Optical system UIS2 (UIS) (Universal Infinity System) optical system Reflected light Reflected light illuminator (BX-RLA2), tube magnification 1X, super illumination widefield (FN 26.5) compatible. Available observations: @ Reflected light brightfield ² Reflected light darkfield ³ Reflected light Nomarski DIC...

  • Page 24: Optical Characteristics <

    (as shown in the diagram on the right). UIS marking NA (Numerical of Aperture) NOTE Brightfield/darkfield Refer to the latest catalogue or consult Olympus for the updated infor- application mation on the eyepieces and objectives that can be combined with this unit. Cover glass thickness FN (Field Number) —: May be used with our with-...
  • Page 25 BX-RLA2 Eyepieces Optical Cover characteristics Resolu- WHN10X (FN22) SWH10X (FN26.5) glass Magnifi- W.D. tion N.A. thick cation (mm) Depth Field Depth Field (µm) Total Total ness of focus of view of focus of view mag. mag. (mm) (µm) (mm) (µm)
  • Page 26 Eyepieces Optical Cover characteristics Resolu- WHN10X (FN22) SWH10X (FN26.5) glass Magnifi- W.D. tion N.A. thick cation (mm) Depth Field Depth Field (µm) Total Total ness of focus of view of focus of view mag. mag. (mm) (µm) (mm) (µm) (mm) Series Marking 20X 0.60...
  • Page 27 BX-RLA2 Significance of Objective Name (Examples) (Plan) None : Brightfield : Darkfield BDP : Brightfield or polarized : IR light Figure : Magnification None : UIS : UIS 2 None : Achromat, or aberration correction with 2 wavelengths (red and bleu).
  • Page 28 Glossary of Terms Used in the Optical Characteristics Table Working distance (WD) : The distance from the top of specimen and the front lens of objective. Number of aperture (NA) : Important figure determining the objective characteristics (resolution, focal depth and bright- ness).

  • Page 29: Assembly

    BX-RLA2 ASSEMBLY }The following diagram is applicable only to the BX51RF/BX51TRF microscopes. For the BXFM-F focusing unit, please refer to its instruction manual. Reflected light brightfield/ darkfield illuminator Rotary wafer holder BX-RLA2 BH2-WHR43 Stage plate U-MSSP4* Revolving nosepiece U-5RE-2 U-D6RE...
  • Page 30 Attaching the Illuminator (Fig. 13) ² }Attach the illuminator using the exclusive 4 mm Allen wrench ( provided with it. }Remove the caps of the mounting screws ² using tweezers, etc. 1. Place the illuminator @ on the mounting surface by slightly deviating the ³...
  • Page 32 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany One Corporate Drive, Orangeburg, NY 10962, U.S.A. 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 31 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia. 6100 Blue Lagoon Drive, Suite 390 Miami, FL 33126-2087, U.S.A.

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