Observation Procedure - Olympus BX-URA2 Instruction Manual

3. With the U-DICRH or U-DICRHC slider that does not have the slide lever,
the applicable objectives are as follows.
DIC Slider
UIS2
U-DICRH
UIS
UIS2
U-DICRHC
UIS
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Observation Procedure

1. Place the specimen on the stage and move the stage to bring the speci-
men into focus.
2. Adjust the field iris diaphragm until it circumscribes the field of view.
3. Stopping down the aperture iris diaphragm may increase the contrast
somewhat.
U-DICR U-DICRHC
1. Rotate the prism control knob | for the DIC prism to adjust the back-
ground contrast as outlined below.
2. Rotating the prism control knob of the DIC prism will continuously change
the interference color of the background from the gray sensitive color to
magenta sensitive color (-100 to 600 nm). Select the interference color
offering optimum contrast for each specimen.
· If the background color is gray, a 3D-looking observation with good con-
trast is possible in the most sensitive gray colors.
· If the background color is sensitive magenta, even a minor optical retar-
dation can be observed as a color change.
U-DICRH
1. Rotate the prism control knob | for the DIC prism to adjust the back-
ground contrast as outlined below.
2. Rotating the prism control knob of the U-DICRH DIC prism will continu-
ously change the interference color of the background from -100 to 100
nm. Select the retardation offering optimum contrast.
· If the background color is gray, a 3D-looking observation with good con-
trast is possible in the most sensitive gray colors.
· If the background color is sensitive magenta, even a minor optical retar-
dation can be observed as a color change.
To use the background color sensitive magenta, use the U-.POTP3 polar-
izer. Position the polarizer so that the symbol can be seen from the front
when the polarizer is inserted into the inlet slot.
# Care should be taken to keep the specimen surface clan, as even a
small amount of contamination on the surface may show up due to
the exceptionally high sensitivity of the DIC method.
}Since the detection sensitivity is variable depending on orientation, it is
recommended to use a rotary stage.
Applicable Objectives
MPLFLN/MPLFLN-BD series
UMPlanFl/UMPlanFl-BD series
MPlanFl-BD series
MPlanApo20X, 100X
LMPLFLN/LMPLFLN-BD series
LMPlanFl/LMPlanFl-BD series
LMPlanApo/LMPlanApo-BD series
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