Troubleshooting Guide - Olympus BX-URA2 Instruction Manual

Reflected fluorescence system
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TROUBLESHOOTING GUIDE

Reflected Light Observation Modes
Problem
a) Bulb operates, but field of view re-
mains dark.
b) Field of view is obscured or not
evenly illuminated.
c) Image glares.
d) Visibility is poor.
· Image is not sharp.
· Contrast is poor.
· Details are indistinct.
e) One side of image is blurred.
Cause
Reflected light lamp is not on.
Aperture and field iris diaphragms are not
opened wide enough.
Mirror unit is not mounted.
Mirror unit is not correctly engaged in
light path.
Optimum mirror unit for observation is
not engaged in light path.
Field iris diaphragm has not been cen-
tered.
Field iris diaphragm is stopped down too
far.
Mercury burner is not centered correctly. Center mercury burner.
Frost filter is not engaged in light path.
Filter is not in click position.
Aperture iris diaphragm is stopped down
too far.
A non-UIS2/UIS objective is used.
Front lens of objective is dirty.
Immersion oil is not being used with an
oil immersion objective.
Recommended immersion oil is not
used.
Light shield tube is not attached.
Specimen is tilted.
Revolving nosepiece is not correctly
mounted.
Objective is not correctly engaged in light
path.
Remedy
Turn lamp on.
Enlarge them to proper sizes.
Mount mirror unit.
Engage mirror unit correctly in light
path.
Set turret so that optimum mirror unit
for observation is engaged in light path.
Center field iris diaphragm/
Enlarge field iris diaphragm until it cir-
cumscribes field of view.
Engage frost filter in light path.
Push filter until it clicks properly.
Open aperture iris diaphragm.
Use only UIS2/UIS series objectives
with this microscope.
Clean objective.
Use immersion oil.
Use provided immersion oil.
Attach light shield tube.
Place specimen properly on stage and
fix with specimen holders.
Attach revolving nosepiece correctly.
Engage objective correctly in light
path.
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