Differential Interference Contrast Microscopy - Nikon Eclipse Ni-U Instruction Manual

Differential Interference Contrast Microscopy

1.3
Problem
The universal condenser's turret is at the
intermediate position.
The DIC slider is at the intermediate position for
an oil condenser.
The DIC module on the objective is at the
intermediate position.
Lack of visibility around
periphery of field of view.
Incomplete attachment of nosepiece.
The polarizer and an analyzer are at the
intermediate position.
The lambda plate is at the intermediate position.
The polarizer is out of the optical path.
The analyzer is out of the optical path.
A correct DIC module on the condenser has not
been selected.
No contrast.
The DIC slider on the objective is out of the
optical path.
Incorrect combination of the objective and the
DIC slider on the objective.
The immersion oil is not sufficient in the oil
condenser, and air bubbles are between the
slide and the condenser.
Chapter 2
Troubleshooting
Cause
31
Measure
Rotate it to the click stop position.
(→See Chapter 3 "7.2 Using the NI-CUD Universal
Condenser (Dry)" in the "Operation".)
Push in the slider until it reaches the limit.
(→See Chapter 3 "7.3 Using the D-CUO DIC Condenser
(Oil)" in the "Operation".)
Attach it correctly.
(→See Chapter 3 "15.2 Using Optical Elements" in the
"Operation".)
Attach it correctly.
(→See Chapter 1, "3 Assembly Method, 14 Attach the
nosepiece" in the "Assembly/Maintenance".)
Switch over correctly.
(→See Chapter 3 "15.2 Using Optical Elements" in the
"Operation".)
Make sure that it is attached to the limit.
(→See Chapter 3 "15.2 Using Optical Elements" in the
"Operation".)
Bring the polarizer into the optical path.
(→See Chapter 2 "4 Differential Interference Contrast
Microscopy - 14 Adjust the orientation (vibration direction)
of the polarizer and analyzer" in the "Operation".)
Bring the analyzer into the optical path.
(→See Chapter 2 "4 Differential Interference Contrast
Microscopy - 14 Adjust the orientation (vibration direction)
of the polarizer and analyzer" in the "Operation".)
Select the DIC module suitable for the objective
used.
(→See Chapter 1 "3 Assembly Method - 13 Attach the
condenser, ■ Attaching the optical module to the universal
condenser", and "■Attaching the DIC module to DIC
condenser oil" in the "Assembly/Maintenance".)
Bring the DIC slider on the objective into the optical
path.
(→See Chapter 2 "4 Differential Interference Contrast
Microscopy - 16 Attach the DIC slider (on the objective) to
the nosepiece" in the "Operation".)
Use the DIC slider suitable for the objective.
(→See Chapter 1 "3 Assembly Method - 13 Attach the
condenser, ■ Attaching the optical module to the universal
condenser", and "■Attaching the DIC module to DIC
condenser oil" in the "Assembly/Maintenance".)
Apply the immersion oil again.
(→See Chapter 3 "12 Oil Immersion/Water Immersion" in
the "Operation".)