Optical System And Operation; General - Nikon Eclipse Ni-U Instruction Manual

1

Optical System and Operation

General

1.1
Problem
Dirt or dust rotates when the eyepiece is turned.
Dirt or dust does not rotate when eyepiece is
turned
(1) The specimen is dirty if dirt or dust moves
(2) The tip of the condenser lens is dirty if dirt
Dirty or dusty field of view
when looking into eyepiece.
(3) The objective is dirty if dirt or dust
(4) The field diaphragm image is not focused
(5) An aperture diaphragm is stopped down
Dirt or dust on the monitor moves when the
camera is turned
Dirt or dust appears on the
monitor.
Dirt or dust on the monitor does not move when
the camera is turned
No cover glass is attached.
The thickness of the cover glass is inadequate.
A high magnification objective appropriate for
hemocytometer cover glass thickness (0.4 mm,
0.7 mm) is not used.
Image quality is poor.
Contrast is poor. Resolution
The objective correction ring does not match the
is poor.
thickness of the cover glass. (for the objective
with a correction ring)
The lens and specimen are dirty or dusty.
Chapter 2
Troubleshooting
Cause
The eyepiece is dirty.
(1) to (5)
when the specimen is moved on stage.
or dust goes in and out of view when the
condenser is moved up and down while
using a low magnification objective.
disappears when the objective is switched.
on the specimen surface. (Condenser
adjustment is incorrect)
too far.
Lens or specimen is dirty or dusty.
The camera is dirty.
26
Measure
Clean the eyepiece.
(→See Chapter 3, "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
(1) Clean the specimen.
(2) Clean the condenser.
(→See Chapter 3, "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
(3) Clean the objective.
(→See Chapter 3, "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
(4) Make sure the condenser is focused and
centered.
(See Chapter 3, "5 Focusing and Centering the Condenser"
in the "Operation".)
(5) Open it to proper size.
(→See Chapter 3, "6 Adjusting the Aperture Diaphragm" in
the "Operation".)
Check and clean it in accordance with the "Dirt or dust
does not rotate when eyepiece is turned" of "Dirty or
dusty field of view when looking into eyepiece".
Remove the camera and clean it in accordance with
the camera's instruction manual.
Attach a cover glass of the specified thickness (0.17
mm). (However, no cover glass is required for an
NCG objective.)
Use the high magnification objective (e.g. CFI LWD
40xC) appropriate for observation using thick cover
glass.
Correct the ring as appropriate.
Check and clean them in accordance with "Dirt or
dust does not rotate when eyepiece is turned" of
"Dirty or dusty field of view when looking into
eyepiece".