Epi-Fluorescence Microscopy - Nikon Eclipse Ni-U Instruction Manual

Epi-fluorescence Microscopy

1.2
Problem
Lack of visibility around
periphery of field of view.
Illumination is uneven The filter cube is misaligned.
across the field of view.
Field of view is not visible.
The shutter is closed.
A fluorescent image is not
visible (when the lamp is
ON).
The selection of the filter cube is incorrect.
The ND filters of the epi-fluorescence
attachment are in the optical path.
The fluorescent image is
very dark (when the lamp is
A halogen light source is used for a dark
ON).
specimen.
A designated objective is not used at UV or V
excitation.
The room is bright.
The dia-illumination lamp is on.
The filter cube being used is not suitable for the
The fluorescent image specimen.
quality is poor.
The objective or cover glass is dirty.
The immersion oil is fluorescent.
The contrast of the
fluorescent image is poor.
The slide glass is fluorescent.
Stray light is entering from the condenser.
Chapter 2
Troubleshooting
Cause
30
Measure
Push the cube in to the limit.
(→See Chapter 1 "3 Assembly Method, 6 Attach the
epi-fluorescence cube turret and epi-fluorescence
attachment - ■ Attaching a filter cube" in the
"Assembly/Maintenance".)
Open the shutter.
(→See Chapter 3 "14.3 Protecting the Sample and
Preventing It from Decoloration (Using the Shutter)" in the
"Operation".)
Use a correct filter cube.
(→See Chapter 3 "14.2 Selecting Filters" in the
"Operation".)
Remove the ND filters from the optical path as
necessary.
(→See Chapter 3 "14.4 Adjusting the Brightness of the
Fluorescent Image (Using ND Filters and the Aperture
Diaphragm)" in the "Operation".)
Change the light source to a mercury lamp.
Use a designated objective.
Make it darker.
Turn off the dia-illumination lamp.
Use a filter cube suitable for the specimen.
(→See Chapter 3 "14.2 Selecting Filters" in the
"Operation".)
Clean it as appropriate.
(→See Chapter 3, "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
Use the non-fluorescent immersion oil.
(→See Chapter 3 "14.7 Other Considerations Concerning
Epi-Fluorescence Microscopy" in the "Operation".)
Use a non-fluorescent slide glass.
(→See Chapter 3 "14.7 Other Considerations Concerning
Epi-Fluorescence Microscopy" in the "Operation".)
Lower the condenser, or remove the condenser and
attach a shielding tube.