Nikon Eclipse Ni-U Instruction Manual page 36

Problem
Parts are attached incorrectly.
Lack of visibility around
Movable part of manually operated unit is not
periphery of field of view.
switched correctly.
Illumination is uneven
across the field of view.
Field diaphragm image is not focused on the
specimen surface.
Field of view is not visible.
Field diaphragm is stopped down too far.
Incorrect combination of the objective with the
condenser.
When using objectives with 4x or less, 2-4x
Lack of visibility around
auxiliary lens of universal condenser is not in
periphery of field of view.
the optical path or condenser switching such as
swing-out or sliding operation is not performed.
Illumination is uneven
across the field of view.
Field of view is not visible. The lamp is attached incorrectly.
The lens and specimen are dirty or dusty.
The specimen is upside down.
Out of focus with an
The thickness of the cover glass is inadequate.
objective of high
magnification.
Fail-safe device for specimen damage
protection of the objective is pushed in.
Objective lens is attached incorrectly.
A focal deviation is high
when switching over
objectives.
Diopter adjustment has not been performed.
The stage is attached incorrectly.
Image is not in focus
although the stage is raised
to the highest position.
The refocusing position is set lower than the
focusing position.
Chapter 2
Troubleshooting
Cause
28
Measure
Confirm that parts (nosepiece, condenser, etc.) are
correctly attached.
(→See Chapter 1, "3 Assembly Method" in the
"Assembly/Maintenance".)
Correctly set the optical path switching knob,
nosepiece, filter cube switchover turret, condenser
turret, and slider, etc. (Move the part until it clicks.)
Make sure the condenser is focused and centered.
(See Chapter 3, "5 Focusing and Centering the Condenser"
in the "Operation".)
Open the field diaphragm slightly wider than the
field of view.
(→See Chapter 3, "8 Adjusting the Field Diaphragm" in the
"Operation".)
Adopt an appropriate combination.
(→See Chapter 3, "7.1 Compatibility of Condenser Type
and Objective Magnification" in the "Operation".)
Bring 2-4x auxiliary lens of universal condenser in
optical path.
For other condensers, swing-out or slide the
condenser.
Attach it correctly.
(→See Chapter 1, "3 Assembly Method, 3 Attach the lamp"
in the "Assembly/Maintenance".)
Clean them as appropriate.
(→See Chapter 3, "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
Turn up the cover glass and attach it to the stage.
(→See Chapter 2 "2 Bright/Dark-field Microscopy - 7 Place
a specimen on the stage, and move the stage to bring the
target into view" in the "Operation".)
Attach a cover glass of the specified thickness (0.17
mm).
Some objective has a stopper to keep the pushed in
state. Turn the tip of the object to release.
If the objective does not have a stopper, do not pull
because the tip cannot be turned. In this case,
contact your nearest Nikon representative.
Screw the objective all the way in.
(→See Chapter 1, "3 Assembly Method - 15 Attach the
objective" in the "Assembly/Maintenance".)
Perform diopter adjustment.
(→See Chapter 3 "4 Adjusting the Diopter" in the
"Operation".)
Attach it correctly.
(→See Chapter 1, "3 Assembly Method - 12 Attach the
stage" in the "Assembly/Maintenance".)
Loosen the coarse focus clamp ring to the limit
which clamps the refocusing mechanism.
(→See Chapter 3 "2.3 Refocusing" in the "Operation".)