Nikon Eclipse Ni-U Instruction Manual page 37

Problem
The nosepiece is not attached correctly, or has
not been fully rotated to the click stop position.
The specimen is tilted relative to the stage
One side of the field of view
surface.
(up, down, right, or left) is
not focused.
The image flows (i.e.
The stage is tilted.
becomes asymmetrically
defocused when moving
the focal point)
The substage is tilted.
The condenser is tilted.
Interpupillary adjustment has not been
performed.
Images in right and left
eyepieces are not
coincident.
Diopter adjustment has not been performed.
Diopter adjustment has not been performed.
Eyes become fatigued.
Brightness is inadequate.
The specimen holder is not securely-fixed to the
stage.
The specimen does not
move smoothly.
Rotating torque of the stage knob is set too
heavy.
Rotating torque of the coarse focus knob is set
Difficult to focus.
too heavy.
The optical path does not
switch when the
The quadrocular tilting tube is fastened.
quadrocular tilting tube is
used.
Chapter 2
Troubleshooting
Cause
29
Measure
Attach it correctly and rotate it to the click stop
position.
(→See Chapter 1, "3 Assembly Method, 14 Attach the
nosepiece" in the "Assembly/Maintenance".)
Position the specimen in place on the stage.
(→See Chapter 2 "2 Bright/Dark-field Microscopy - 7 Place
a specimen on the stage, and move the stage to bring the
target into view" in the "Operation".)
Attach the stage correctly.
(→See Chapter 1, "3 Assembly Method - 12 Attach the
stage" in the "Assembly/Maintenance".)
Attach the substage correctly.
(→See Chapter 1, "3 Assembly Method, 10 Attach the
substage" in the "Assembly/Maintenance".)
Attach the condenser securely.
(→See Chapter 1, "3 Assembly Method, 13 Attach the
condenser" in the "Assembly/Maintenance".)
Perform interpupillary adjustment.
(→See Chapter 2 "2 Bright/Dark-field Microscopy - 10
Adjust the interpupillary distance" in the "Operation".)
Perform diopter adjustment.
(→See Chapter 3 "4 Adjusting the Diopter" in the
"Operation".)
Perform diopter adjustment.
(→See Chapter 3 "4 Adjusting the Diopter" in the
"Operation".)
Adjust the brightness using the brightness control
knob or ND filters to attain a suitable brightness.
(See Chapter 3, "1 Adjusting the Brightness of a Diascopic
Image" in the "Operation".)
Fix the holder securely.
(→See Chapter 2 "2 Bright/Dark-field Microscopy - 7 Place
a specimen on the stage, and move the stage to bring the
target into view" in the "Operation".)
Adjust to the appropriate torque weight.
(→See Chapter 3 "3 Bringing the Target into the Optical
Path - Adjusting the knob rotation torque" in the
"Operation".)
Adjust to the appropriate torque weight.
(→See Chapter 3 "2.2 Focus Knobs on the Main
Body - Adjusting the rotating torque of the coarse focus
knob" in the "Operation".)
Unfasten it. (Remove the tightening screw for the
quadrocular tilting tube.)
(→See Chapter 1 "3 Assembly Method - 7 Attach
the quadrocular tilting tube and DSC zooming port
for quadrocular tube" in the
"Assembly/Maintenance".)