Nikon Eclipse Ni-U Instruction Manual page 35

Problem
An aperture diaphragm is stopped down too far.
Otherwise, it is open too much.
The field diaphragm image is not focused on the
specimen surface.
(Condenser adjustment is incorrect)
No immersion oil is applied to the tip of an
Image quality is poor.
oil-immersion objective.
Contrast is poor. Resolution
is poor.
The designated immersion oil is not used.
The immersion oil contains air bubbles.
The immersion oil adheres to the tip of the
dry-type objective.
The ND filters are out of the optical path.
Field of view is too bright.
The lamp voltage is too high.
The lamp voltage is too low.
The condenser aperture diaphragm is stopped
down too far.
Field of view is too dark.
The field diaphragm image is not focused on the
specimen surface.
The optical path is not switched to binocular
100%.
The NCB11 filter is not used.
Image is yellowish or very
bluish.
Lamp voltage is too low or too high.
Visually observed image
color does not match color White balance of the camera is not set correctly.
of image on monitor.
A filter cube is in the optical path even though
The entire field of view is
epi-fluorescence observation is not being
bluish or yellowish.
performed.
Chapter 2
Troubleshooting
Cause
27
Measure
Open it to the proper size.
(→See Chapter 3, "6 Adjusting the Aperture Diaphragm" in
the "Operation".)
Make sure the condenser is focused and centered.
(See Chapter 3, "5 Focusing and Centering the Condenser"
in the "Operation".)
Apply our designated non-fluorescent immersion oil.
(→See Chapter 3 "12 Oil Immersion/Water Immersion" in
the "Operation".)
Remove the air bubbles.
(→See Chapter 3 "12 Oil Immersion/Water Immersion" in
the "Operation".)
Clean it as appropriate.
(→See Chapter 3 "12 Oil Immersion/Water Immersion" in
the "Operation".)
Place the ND filters into the optical path.
(→See Chapter 3 "1.2 Adjustment with ND Filters" in the
"Operation".)
Turn the brightness control knob to the
position and adjust the brightness with the ND
filters. (See Chapter 3, "1 Adjusting the Brightness
of a Diascopic Image" in the "Operation".)
Turn the brightness control knob to the
position and adjust the brightness with the ND
filters. (See Chapter 3, "1 Adjusting the Brightness
of a Diascopic Image" in the "Operation".)
This should normally be adjusted to 70 to 80% of
numerical aperture of the objective.
(→See Chapter 3, "6 Adjusting the Aperture Diaphragm" in
the "Operation".)
Make sure the condenser is focused and centered.
(→See Chapter 3, "5 Focusing and Centering the
Condenser" in the "Operation".)
Set to binocular 100%.
(→See Chapter 3, Section 10 "Switching the Optical Path
of the Tube" in the "Operation".)
Use the NCB11 filter.
(→See Chapter 3 "1.2 Adjustment with ND Filters" in the
"Operation".)
Turn the brightness control knob to the
position and adjust the brightness with ND filters.
(See Chapter 3, "1 Adjusting the Brightness of a Diascopic
Image" in the "Operation".)
Set the white balance in accordance with the
camera's instruction manual.
Remove the filter cube from the optical path.
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