Run A Blanking Cycle - Thermo Scientific NanoDrop One User Manual

Solutions for blanking problems
• Thoroughly clean and/or
then:
– rerun blanking cycle, or
– measure new blank using new aliquot of appropriate
buffer solution, then measure new aliquot of unknown
sample
• For most applications, blank with the same buffer solution
used to resuspend the analyte of interest. The blanking
solution should be a similar pH and ionic strength as the
analyte solution. For details, see "To measure samples" in
the application used.
• If blanking problems persists, use an application that may
be more suitable such as a fluorescence assay using the
NanoDrop 3300 or a colorimetric assay if measuring
proteins.

Run a Blanking Cycle

Run a blanking cycle to verify the following:
Supplies needed

For quick demonstration, watch multimedia training
Suitability.
Thermo Scientific
recondition both pedestals
• instrument is operating normally (with flat baseline)
• pedestals are clean (i.e., no dried-down sample material on pedestals)
• absorbance contribution of buffer solution you plan to use for sample analyses
• lint-free laboratory wipes
• calibrated precision pipettor (0–2 μL)
• buffer solution for evaluation
To run a blanking cycle
and
Evaluating a Blanking Solution for
18
Learning Center
Prepare Samples and Blanks
NanoDrop One User Guide 201