Column Cleaning, Regenerating, And Storage; Cleaning And Regeneration - Waters CORTECS Care And Use Manual

2.7nm columns

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[ CARE AND USE MANUAL ]

IV. COLUMN CLEANING, REGENERATING,

AND STORAGE

a. Cleaning and Regeneration

Changes in peak shape, peak splitting, shoulders on the

peak, shifts in retention, change in resolution, or increasing

backpressure may indicate contamination of the column.

Flushing with a neat organic solvent, taking care not to

precipitate buffers, is usually sufficient to remove the

contaminant. If the flushing procedure does not solve the

problem, purge the column using the following cleaning

and regeneration procedures.

Use the cleaning routine that matches the properties of the

samples and/or what you believe is contaminating the column

(see Table 3). Flush columns with 20-column volumes of

Table 3. Recommended pH and Temperature Limits for CORTECS Columns

Name of

Ligand type

column

Trifunctional C

Phenyl

Trifunctional phenyl

HILIC

None

Table 4. Reversed-Phase Column Cleaning Sequence

Polar Samples

1. Water

2. Methanol

3. Tetrahydrofuran (THF)

4. Methanol

5. Water

6. Mobile phase

* Prior to using THF or hexane, ensure your system is compatible with these solvents. THF or hexane should only be considered when the column cannot be

cleaning by running neat, reversed-phase organic solvents such as acetonitrile. Reduce flow rate, lower operating temperatures, and limit system exposure to

THF and/or hexane.

** Use low organic solvent content to avoid precipitating buffers.

Surface

End cap

charge

style

modification

None

Proprietary

None

Proprietary

None