Related Manuals for Merck Pellicon Capsule
Summary of Contents for Merck Pellicon Capsule
- Page 1 User Guide Pellicon Capsule ® Pellicon Capsule Stand ® The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
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Page 2: Table Of Contents
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Contents Introduction The capsules are self-contained and do not require a compression holder. Sizes 0.1, 0.5 and 1 m may be clamped on a user- Pellicon capsules are a family of single- ®... -
Page 3: Pellicon ® Capsules
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Pellicon Capsules Unpacking ® Pellicon capsules are individually packaged. The packaging includes double bags, foam ® pouches or foam frames, and a corrugated box. Pellicon capsule manifolds in sizes 3 and 4.5 ®... - Page 4 Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Securely grasp the outer plastic bag Place the bagged manifold on a flat Hold the bottom foam piece down and assembly by its edges and lift free surface, cut the inner bag at the seal lift the manifold unit by the handles.
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Page 5: Installation Materials
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Installation Materials Operation Mode Below is a list of recommended materials for Pellicon capsule Product installation and system setup is defined by the TFF operation ® installation: mode: Component Operation Mode Parameter Diaphragm or Peristaltic Pump Feed Pump... - Page 6 Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Batch TFF Installation Batch TFF installations allow the feed to pass through the filter assembly multiple times. If installing multiple devices, assemble for parallel flow using tubing to connect shared feed, retentate, and permate lines. Permeate Permeate to Drain...
- Page 7 Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Single-pass TFF Installation Single-pass TFF installations allow the feed to pass through each filter once to generate permeate and concentrated retentate without recirculation. Single-pass TFF installations use either one or mutiple Pellicon capsules.
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Page 8: Manifold Installation
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Manifold Installation Installation of 1, 3 or 4.5 m of Membrane Area Pellicon capsule manifold assemblies of 1, 3 and 4.5 m of membrane area (manifold formats ® Pellicon capsule manifold assemblies are ®... - Page 9 Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Align the three (3) AseptiQuik L connectors on the opposite side The following procedure requires various connections of AseptiQuik ® ® of the E manifold labeled To Filter to the three (3) AseptiQuik L connectors.
- Page 10 Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® NOTE Do not pull the membrane tabs on the connectors at Insert the center unification bracket provided with the E this step as the flow path installation is not complete. manifold into the space on the inner handles of each manifold plate and push the center unification bracket down until it clicks E Manifold Assembly...
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Page 11: Integrity Testing
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Integrity Testing Air Integrity Test Specifications Ultracel 30 kD Membrane ® Air Flow Rate (cc/min) at 30 psig Optional integrity testing of the Pellicon capsule may be performed ® 0.1 m Capsule 0.5 m Capsule... -
Page 12: Conditioning Flush
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Conditioning Flush Retentate Pellicon capsules are wetted with purified water, then gamma ® sterilized prior to shipment. Flushing removes the storage water present in the shipped product, and can be performed with either water for injection (WFI) or conditioning buffer. -
Page 13: Product Processing
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Product Processing Record the feed and permeate flow rate; feed, retentate, and permeate pressures; and temperature throughout the concentration process. Always avoid stress such as impact and pulling at connection points After reaching the target concentration, fully open the retentate during use. -
Page 14: Pellicon ® Capsule Stand
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Pellicon Capsule Stand ® Installation The Pellicon capsule stand accommodates up to two 0.1 m capsules in parallel or three 0.1 m capsules in series on one side and one 0.5 m ®... -
Page 15: Troubleshooting
Pellicon Capsule and Pellicon Capsule Stand User Guide ® ® Troubleshooting Problem Possible Cause Remedy Replace filter. Determine cause to avoid recurrence. Physical Problem Possible Cause Remedy damage, such as exceeding Low retention Membrane damaged Tubing split or fitting permeate backpressure limits Replace damaged part. - Page 16 Phone: 1-800-645-5476 www.sigma-aldrich.com Millipore, Pellicon and Ultracel are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. © 2022 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
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