Page 2
Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
Safety During Installation and/or Maintenance This centrifuge is designed to be installed by a Beckman Coulter field service representa- tive. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument.
Page 4
Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge. • Do not exceed the maximum rated speed of the rotor in use. • NEVER attempt to slow or stop a rotor by hand.
ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.
Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual is the sole responsibility of the user.
Introduction WARNING Used whenever an action or condition may potentially cause personal injury or loss of life. Mechanical damage may also result. WARNING Indicates high voltage or risk of electric shock. Refer servicing of all areas displaying either symbol to qualified service personnel. TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and...
For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment,...
Specifications and Preinstallation Requirements SPECIFICATIONS Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. CONTROL FEATURES Speed Setting range... . 100 to 26 000 rpm (in 10-rpm increments) or equivalent RCF (in 100 ×...
Specifications and Preinstallation Requirements Deceleration ....... maximum, slow, timed (1 to 10 min from 500 to 0 rpm), or off OPERATIONAL FEATURES Door.
4°C minimum at 21 500 rpm (30°C ambient). Maximum speed for the JA-30.50 Ti rotor is 26 000 rpm in the Avanti J-26XPI centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge. Temperature performance for the JA-25.50 and JA-25.15 rotors in the Avanti J-26 XPI is as follows: 10°C minimum @ 25 000 rpm (at 30°C ambient);...
Page 18
4°C minimum at 21 500 rpm (30°C ambient). Maximum speed for the JA-30.50 Ti rotor is 26 000 rpm in the Avanti J-26XPI centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge. Temperature performance for the JA-25.50 and JA-25.15 rotors in the Avanti J-26 XPI is as follows: 10°C minimum @ 25 000 rpm (at 30°C ambient);...
Page 19
4°C minimum at 21 500 rpm (30°C ambient). Maximum speed for the JA-30.50 Ti rotor is 26 000 rpm in the Avanti J-26XPI centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge. Temperature performance for the JA-25.50 and JA-25.15 rotors in the Avanti J-26 XPI is as follows: 10°C minimum @ 25 000 rpm (at 30°C ambient);...
Page 20
4°C minimum at 21 500 rpm (30°C ambient). Maximum speed for the JA-30.50 Ti rotor is 26 000 rpm in the Avanti J-26XPI centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge. Temperature performance for the JA-25.50 and JA-25.15 rotors in the Avanti J-26 XPI is as follows: 10°C minimum @ 25 000 rpm (at 30°C ambient);...
Page 21
4°C minimum at 21 500 rpm (30°C ambient). Maximum speed for the JA-30.50 Ti rotor is 26 000 rpm in the Avanti J-26XPI centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge. Temperature performance for the JA-25.50 and JA-25.15 rotors in the Avanti J-26 XPI is as follows: 10°C minimum @ 25 000 rpm (at 30°C ambient);...
Specifications and Preinstallation Requirements PREINSTALLATION REQUIREMENTS Do not attempt to install this instrument. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than an authorized Beckman Coulter representative invalidates any warranty covering the instrument. Preinstallation requirements have been sent prior to shipment of the instrument.
Page 23
Specifications and Preinstallation Requirements Table 1-1. Nominal Supply Voltage Ratings Instrument Instrument Part Number Nominal Instrument Part (Elutriation Nominal Power Cord and Plug Voltage Rating Number System) Supply Frequency Description Single-phase, 393127 393133 180–264 VAC, permanently attached three- 200/208/240 V 50/60 Hz, 30 A wire UL/CSA-approved cord with NEMA type 6-30P plug...
Specifications and Preinstallation Requirements Additional Requirements for Three-phase Power Connections For three-phase power service to the centrifuge, use the “Y” connected configuration shown in Figure 1-3. Also note the following information: • The steady state current draw of the centrifuge can be as high as 12 amperes per phase, depending on the voltage.
Specifications and Preinstallation Requirements • In addition to space for the centrifuge, allow a 7.7-cm (3-in.) clearance on each side of the centrifuge and a 16-cm (6.25-in.) clearance behind the centrifuge for air circulation. The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during centrifuge operation.
Refer to this document for additional installation instructions. ➠ NOTE Beckman Coulter representatives are not equipped to drill holes in your floor. The holes must be drilled before your scheduled installation.
Avanti J. To check for drive pins, hold the rotor up or turn it on its side and look into the drive hole. If you do not see two metal pins in the hole, call your local Beckman Coulter office for information on returning the rotor to the factory for upgrading.
Specifications and Preinstallation Requirements Rotor Drive Drive Pins Hole (Angled pins shown; pins can also be vertical or horizontal.) Drive Spindle Assembly Top View Side View Figure 1-5. Checking the Rotor for Drive Pins USING THE JCF-Z CONTINUOUS FLOW/ZONAL ROTOR Zonal Bracket Kits A special bracket and mounting hardware are required when the JCF-Z Continuous Flow/Zonal Rotor is used.
Page 30
The excess lubrication produces drag on the bearings, which can change the rotor’s dynamic properties enough to cause rotor misidentification. To wear in the bearings and distribute In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. 1-16...
Page 31
Specifications and Preinstallation Requirements the lubrication, perform three runs from 0 to 5000 rpm and back to 0 rpm. ➠ NOTE If the JCF-Z rotor is misidentified, the run speed will be limited to the maximum speed for the identified rotor. (The maximum speed for the JCF-Z rotor is 20 000 rpm.) 1-17...
CENTRIFUGE FUNCTION AND SAFETY FEATURES CENTRIFUGE FUNCTION The Avanti J-26XPI is a refrigerated centrifuge that generates centrif- ugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, appli- cations include: •...
Description • Dynamic Rotor Inertia Check (DRIC): As the rotor accelerates, rotor inertia is measured and the rotor energy is calculated for the speed set by the user. If the calculated rotor energy is determined to be excessive, the centrifuge recalculates a permitted set speed and uses this value to avoid possible rotor damage.
Description FRICTION REDUCTION SYSTEM (FRS) The friction reduction system (FRS) uses a mechanical rotary vane vacuum pump to reduce chamber pressure to approximately one- quarter atmosphere (190 mm Hg). The pump turns on after the run is started, before rotor friction reaches a high level. When the required vacuum level is reached, the pump turns off.
NAME RATING PLATE The name rating plate is affixed to the rear of the centrifuge. Always mention the model number and serial number when corresponding with Beckman Coulter regarding your centrifuge. CONTROLS AND INDICATORS KEY AND POWER SWITCHES The key switch and the power switch are located below the control panel (see Figure 2-1).
Description • RUN LOCK mode disables all user inputs except [ ] and START ] to ensure that current settings do not get changed. STOP • INST(RUMENT) LOCK mode disables all user inputs except ], to prevent use of the instrument after the current run is STOP complete.
Description ROTOR ID SPEED TIME TEMP RPM/RCF HR:MIN °C Figure 2-3. The Actual Value Display Indicates the installed rotor. At the start of a run, this field is blank. ROTOR ID During acceleration, the centrifuge identifies the installed rotor and displays the rotor name.
Description Rotor : JA-30.50 Speed : - - - Time : 00:00 Temp : 25°C Max Temp : 29°C Soft Function Accel : MAX Decel : Keys Keys Message Line Figure 2-4. The Setup Screen When the centrifuge power is turned on, the setup screen displays the parameters of the last run performed.
Page 41
Description • Timed run (HH:MM) — Run time up to 99 hours and 59 minutes can be set. If more than 59 minutes are entered in the minutes field, the system automatically converts the entry to hours and minutes. Decelerating begins when the set time counts down to zero. The corresponding ω...
Description Pressed to put the centrifuge into program mode, where you can enter run parameters into memory for later use. Up to thirty two-step programs can be stored. KEYPAD The keypad is used to enter numerical run parameters and to enter or recall a program number.
Operation This section contains detailed centrifuge operating procedures. A summary is provided at the start of this section. If you are an experi- enced user of this centrifuge, you can turn to the summary for a quick review of operating steps. WARNING Normal operation may involve the use of solutions and test samples that are patho-...
Operation SUMMARY OF AVANTI J-26XPI RUN PROCEDURES For runs at other than room temperature, precool or prewarm the rotor to the required temperature before the run. MANUAL RUN Press the POWER switch to on (I). Depress the foot pedal to open the chamber door.
Operation PROGRAMMED RUN Press the POWER switch to on (I). Depress the foot pedal to open the chamber door. Install the rotor according to instructions in the applicable rotor manual, then firmly close the chamber door. Press [ ], then use the keypad to enter the program number. Check that all parameters are correct and that the door is closed.
Operation Action Result CAUTION 3. Install the rotor according Do not drop the rotor onto the drive hub. The to the instructions in the drive spindle can be bent if the rotor is forced rotor manual. Make sure sideways or dropped onto the spindle hub. that the rotor is seated on Install the rotor by centering it over the hub the drive hub.
Operation SELECTING A ROTOR Action Result 1. Press [ The Rotor: field flashes, and rotor type abbreviation soft keys ROTOR (JA: fixed angle, JS: swinging bucket, JLA: lightweight fixed angle; JSP: special; JV: vertical tube; JE: elutriator; JCF-Z: continuous flow/ zonal) appear.
Page 48
Operation Entering RPM Action Result 1. Press [ The Speed: field flashes, and RPM and RCF soft keys appear. The SPEED RPM indicator illuminates in the actual value display. ROTOR ID SPEED TIME TEMP RPM/RCF HR:MIN °C Rotor : JA-30.50 Speed : - - -- x G Time :...
Operation Action Result 2. Press the RCF soft key. The RPM = - - × G field flashes. ROTOR ID SPEED TIME TEMP RPM/RCF HR:MIN °C RCF (x g) Rotor : JA-30.50 Speed : - - = 81 800 x G Time : 00:00 Temp : 25°C Max Temp : 29°C...
Page 50
Operation Action Result 2. Use they keypad to enter Entered time appears in the Time: field. the required time (up to 99 hours, 59 minutes) and press [ ENTER Rotor : JA-30.50 HH:MM Speed : 26 000 Time : 00:25 HOLD Temp : 25°C Max Temp : 29°C...
Page 51
Operation Action Result 3. Use the keypad to enter an The entry appears in the Time: field. The decimal point and E appear ω t value. automatically as you type. If you enter a value out of the achievable ω t range for that rotor, a message prompts you to enter a different value.
Operation Action Result 2. Press the HOLD soft key HOLD appears in the Time: field. to select the hold mode. ROTOR ID SPEED TIME TEMP RPM/RCF HR:MIN °C HOLD HH:MM Rotor : JA-30.50 Speed : 26 000 81 800 x G HOLD Time : 00:00 Temp : 25°C...
Operation Action Result 2. Use the keypad to enter the Entered temperature appears in the Temp: field. required temperature. 3. Press the [ ] key again Entered temperature plus 4°C appears in the Max Temp: field, and TEMP to move the cursor to the YES and NO soft keys appear.
Operation Table 3-1. Acceleration and Deceleration Settings Setting Description Maximum acceleration Full torque is used from 0 rpm until set speed is reached. (not available with the JS-24.38 and JS-24.15 rotors) Slow acceleration Approximately 2 minutes from 0 to 500 rpm. (Time depends on the mass of the rotor in use;...
Operation Deceleration Action Result 1. Press [ ] twice (if the The Decel: field flashes. MAX, SLOW, TIME, and OFF soft keys cursor is in the Accel: field, appear. press [ ] once). 2. Press the appropriate If you choose MAX, SLOW, or OFF, your selection appears in the soft key.
Operation ENDING A RUN To end a run in progress, press [ ]. The red [ ] indicator light STOP STOP flashes until the rotor comes to a complete stop. In timed runs, deceleration begins automatically when the TIME ω display counts down to zero.
Page 57
Operation Action Result 2. Press [ ] again to move the A two-step program is created and is assigned the number you cursor to the Setup: field. entered. If you enter a number that is already assigned to a program, Use the keypad to enter a you can either overwrite the program or select another program program number in this field,...
Operation Action Result 5. To add a second step to program 1, press the STEP2 soft key. 6. Enter parameters for step 2. All parameters for step 1 and 2 are saved under the selected program Press [ ] when done. number.
Page 59
Operation Action Result 2. Press [ ] again to move the The program number appears in the Setup: field. A message appears cursor to the Setup: field. asking if you want to overwrite the program. YES and NO soft keys Enter the number of the appear.
Operation RUNNING A PROGRAM Action Result 1. Press [ The Prog Recall: field flashes. STEP1 Rotor : JA-30.50 STEP2 Speed : 26 000 81 800 EXIT Time : 00:20 Temp : 20°C Max Temp : 24°C Shutdown? No Accel : 5 Decel : 5 Prog Recall: 1 Setup:...
Page 61
Operation Action Result 1. Turn the key to the Open-door operation up to 3000 rpm is enabled. JCF-Z appears in the ZONAL position. Rotor: field. ZONAL Rotor : JCF-Z JCF-Z Speed : 0 Time : 00:00 Temp : 25°C Max Temp : 29°C Accel : MAX Decel : MAX 2.
Operation Action Result WARNING 8. Open the door and unload Never try to slow or stop the rotor by hand. the rotor. ➠ NOTE 9. Press [ ] to bring the Before another zonal run can be started, you STOP rotor to a complete stop.
Page 63
Operation Action Result 1. Install the rotor. 2. Set up sample and buffer reservoirs and tubing lines as described in the rotor manual. Route tubing lines through the elutriator port holes at the left side of the door seal. Close the centrifuge door. 3.
Page 64
Operation Action Result 7. When the rotor speed has stabilized, look through the port in the centrifuge door and turn the control DELAY DELAY Delay knob until the elutriation Control ELUTRIATOR Knob chamber in the rotor is synchronized with the strobe. Sample can now be injected into the lines.
Beckman Coulter Field Service representative (in the United States, call 1-800-742-2345; outside the U.S., call your local Beckman Coulter office). To help the field service representative diagnose and correct the problem, try to gather as much information about the situation as you can,...
If this does not work, call Beckman to manual of run Coulter Field Service. Door problem, refer to manual In the U.S. call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. Continued —...
Page 67
FRS level too high Run shuts down with Call Beckman Coulter Field FRS failure, refer to maximum brake Service. manual * In the U.S. call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. Continued — † Friction Reduction System.
Page 68
Message Problem Result Recommended Action There is a problem with System shuts down Call Beckman Coulter Field Service. System error the system control soft- ware, EPROM, or RAM Press [ ] (green light on [ a) a run is in progress...
Troubleshooting ACCESSING THE ROTOR IN CASE OF POWER FAILURE WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power off (O) and disconnect the instrument from the main power source, and refer such main- tenance to qualified service personnel.
Troubleshooting Figure 4-1. Emergency Door Release Latch Access Action Result 2. Insert a 4-mm or -in. The latch disengages. Allen wrench straight through one of the holes (either one) and turn the wrench to the left (counter- clockwise) about one-half turn.
Troubleshooting Manual Door Release Interlock Lever Inner Front Panel Retaining Strip Figure 4-2. Manual Door Release Action Result 5. Pull the interlock lever out Depending on the level of vacuum in the chamber at the time of the and to the left (at about a power failure, the door may or may not open.
Page 72
Troubleshooting Action Result 7. To vent the chamber vacuum, The vacuum will be completely vented several seconds after the hose grasp the red rubber hose and is detached. pull it up until it comes off of the pump fitting (see ➠...
Troubleshooting Grasp red hose and pull up. Do not disconnect the tubing lines tied to the red hose. Vacuum Pump Red hose removed from fitting Replace red hose Figure 4-3. Venting the Chamber Vacuum...
Troubleshooting To replace the panel on the centrifuge, Action Result 1. Holding the panel at the top (with the instrument label facing out), insert the three grooved brackets on the bottom inside of the door over the front panel retaining strip (see Figure 4-4).
If the JCF-Z rotor is misidentified, the run speed will be limited to the maximum speed for the identified rotor. (The maximum speed for the JCF-Z rotor is 20 000 rpm.) In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. 4-11...
WARNING Do not use alcohol or other flammable substances in or near operating centrifuges. In the U.S., call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office.
Care and Maintenance MAINTENANCE Perform the following procedures regularly to ensure continued performance and long service life of the centrifuge. • Inspect the centrifuge chamber for accumulations of sample, dust, or glass particles from broken sample tubes. Clean as required (see , below).
IN-175 for chemical resistances of centrifuge and accessory materials. While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge, no guarantee of sterility or disinfec- tion is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.
Then set the bottom edge down. CIRCUIT BREAKER AND FUSES There are no user-replaceable fuses in the Avanti J-26XPI centrifuge. If the circuit breaker trips for any reason, the power switch will move to the OFF (O) position. Reset the circuit breaker by turning the power switch back to the ON (I) position.
Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions.
Page 82
Care and Maintenance Zonal Tubing Adapter for Cole-Parmer size 15 tubing (9.6-mm [ -in.] O.D.) ........363845 Note: kit 366431 is also required.
APPENDIX Temperature Calibration Procedure The Avanti J-26XPI centrifuge specification for temperature control is ±2°C of the set temperature. That means that your sample will stay within 2°C of set temperature at all times, after rotor and system equilibration, described below. (During transient conditions, such as acceleration and deceleration, the rotor temperatures may be outside this range.) The following procedure is provided for those cases in...
Page 84
Temperature Calibration Procedure 5. After at least 1 hour, measure the temperature of the buffer or water using a thermometer or thermocouple. 6. If the measured and set temperatures are different, note how many degrees different the measurements are and adjust the set tempera- ture up or down that number of degrees.
Beckman Coulter, vice for a reasonable period of time, Beckman Coulter will or unless such repair in the sole opinion of Beckman Coulter repair or, at its election, replace such component or accessory.
Need help?
Do you have a question about the Avanti J-26XPI and is the answer not in the manual?
Questions and answers