Cb (e7), co2 incubators, co2 incubators with o2 control, co2 incubators with active humidification.
with sterilizable ndir sensor system for co2 and microprocessor program controller mb2 (175 pages)
Incubators avantgarde.line with natural/forced convection, drying and heating ovens avantgarde.line with natural/forced convection and enhanced timer functions (36 pages)
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9040-0082, 9140-0082 left 115 V C150 9040-0080, 9140-0080 right 100 V C150 9040-0083, 9140-0083 left 100 V BINDER GmbH Address Post office box 102 D-78502 Tuttlingen Tel. +49 7462 2005 0 +49 7462 2005 100 Internet http://www.binder-world.com E-mail info@binder-world.com Service Hotline...
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EG – KONFORMITÄTSERKLÄRUNG EC - DECLARATION OF CONFORMITY CE - DECLARATION DE CONFORMITE BINDER GmbH Anbieter / Supplier / Fournisseur: Im Mittleren Ösch 5, D-78532 Tuttlingen Anschrift / Address / Adresse: -Inkubator Produkt / Product / Produit: Incubator Incubateur à CO...
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(CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006) D-78532 Tuttlingen, 17.05.2011 BINDER GmbH P. M. Binder Dr. H. von Both Geschäftsführender Gesellschafter Leiter F & E Managing Director Director R &...
Contents SAFETY........................7 Legal considerations ...........................7 Structure of the safety instructions......................7 1.2.1 Signal word panel ..........................7 1.2.2 Safety alert symbol ........................8 1.2.3 Pictograms .............................8 1.2.4 Word message panel structure......................9 Localization / position of safety labels at the unit................9 Type plate ............................10 General safety instructions on installing and operating the CO incubator ........12 Intended use .............................13...
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CONTROLLER RP1 SETTINGS ................36 Altitude of the installation site above sea level .................36 Entering the set points of temperature and CO ................38 TEMPERATURE SAFETY DEVICES ..............39 Over temperature protective device (class 1) ...................39 Safety controller (temperature safety device class 3.1)..............39 9.2.1 Setting the safety controller set point type...................39 9.2.2 Setting the safety controller set point...................41 10.
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16.1 Maintenance intervals, service......................65 16.2 Checking the air jacket heating fan....................65 16.3 Gas inlet fine filter ..........................65 16.4 Sending the unit back to BINDER GmbH ..................66 17. DISPOSAL......................66 17.1 Disposal of the transport packing .....................66 17.1.1 Outer unit packing........................66 17.1.2 Packing inside the unit, equipment ....................67...
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam- ages, direct or incidental arising out of or related to the use of this manual.
WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury CAUTION Indicates a potentially hazardous situation, which, if not avoided, may result in damage to the product and/or its functions or to property in its proximity.
Prohibition signs Do NOT touch Do NOT spray with Do NOT climb water Information to be observed in order to ensure optimum function of the product. 1.2.4 Word message panel structure Type / cause of hazard. Possible consequences. ∅ Instruction on how to avoid the hazard: prohibition Instruction on how to avoid the hazard: mandatory action Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.
C 150 -UL (door hinged right) 150-UL (door hinged left) Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER Service for these replacements. Type plate Figure 5: Position of type plate C 150 (E2) 06/2011...
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5405194 / 5601143 / 5773287 / 6079403 D 78532 Tuttlingen / Germany C 150 Serial No. 00-00000 Tel. + 49 (0) 7462/ 2005-0 Internet: www.binder-world.com Made in Germany Figure 6: Type plate C 150 (standard unit) Indications of the type plate...
(for Germany). BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or quali- fied personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
(chap. 16) is part of the intended use. Other applications are not approved. WARNING: If customer should use a BINDER chamber running in non-supervised continu- ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
A highly precise, drift-free CO infrared measuring system in combination with the permanent mixture of gas through a special proprietary gas mixing head developed by BINDER allows precise and con- stant CO concentrations for long periods. This creates optimum growth conditions for cultures. The CO sensor can be removed from the inner chamber by hand and cleaned with suitable detergents if needed.
Unit overview (10) Figure 7: CO incubator C 150 (door hinged right) Triangle instrument panel with RP1 controller for temperature and CO and main power switch (2) Gas mixing head CO sensor Pt 100 temperature sensor Shelves Lower housing cover Silicone measuring port Inner glass door (10) Water pan...
2.3 Connection panel at the rear of the unit (11) (12) (13) (14) (15) Figure 8: Rear connection panel C 150 (11) Power cable (12) Miniature fuse (13) Connection socket for zero-voltage relay alarm contact (14) Quick acting closure socket for CO gas bottle (15) Ethernet interface for computer communication (option) Triangle instrument panel C 150...
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commis- sioning the unit.
Figure 10: Lift the unit with the aid of 4 people • Permissible ambient temperature range for transport: -10 °C / 14°F to +60 °C / 140°F. You can order transport packing and rolling pallets for transportation purposes from BINDER Service. C 150 (E2) 06/2011...
Storage Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for tempo- rary decommissioning (chap. 17.2). • Permissible ambient temperature range for storage: -10 °C / 14°F to +60 °C / 140°F. • Permissible ambient humidity: max. 70% r.H., non-condensing When after storage in a cold location you transfer the unit to its warmer installation site, condensation may form in the inner chamber, on the housing or in the sensor compartment of the CO measurement.
Do not install or operate the CO incubator C 150 in potentially explosive areas. DANGER Explosion hazard. Danger of death. ∅ Do NOT operate the unit in potentially explosive areas. ∅ KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit. is harmful in high concentrations.
sensor Connect or remove the CO sensor without rotating and only when the incubator is turned off. Remove the CO sensor before removing or replacing its filter cap. The Teflon filter of the CO sensor prevents dirt and humidity from intruding into the measuring cell. It is available as a spare part. Replace it whenever it is damaged or soiled.
Water pan The water pan permits high humidity without condensation on the inner walls of the incubator. Place the positioning frame on the two marks at the bot- tom of the inner chamber. Then put the water pan in the positioning frame. Make sure that the water pan has firm contact with the inner chamber bottom and rests...
Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to con- nect the original hose nozzle. In this case, please contact BINDER Service. C 150 (E2) 06/2011...
Remove the rubber cover (b) by pulling it off. Unit rear Figure 15: Connection of the hose lead to the gas bottle Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.
A gas supply pressure above 2.5 bar / 36 psi will result in unit damage. It is thus important to check the real output pressure of gas bottles, sets of gas bottles or central gas supplies before connecting the gas hose to the incubator.
Start up After connecting the supply lines, turn on the unit by the main power switch (2). After turning on the incubator for the first time, enter the altitude of the site above sea level into the con- troller RP1 (chap. 8.1). Warming chambers may release odors in the first few days after commissioning.
C 150 was last used for, for hygiene purposes you should clean and disinfect or sterilize the interior (chap. 15). WARNING: If customer should use a BINDER chamber running in non-supervised continu- ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
Open the CO supply's pressure reducer valve and set a CO primary pressure of 2.0 bar / 29 psi. Set the main power switch (2) to position I. The pilot lamp shows the unit is ready for operation. There is a subsequent brief startup phase in which the display items at the edges of the upper controller display light up successively.
Selecting and setting the operating functions The controller's lower display always shows the operating function (e.g. “SP” = temperature set point) and the upper display shows the associated value (e.g. 37.0). When the value in the upper display shows the temperature in °C or a CO concentration in % CO , the corresponding yellow LED to the right of the display will light up.
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Operating functions that can be set in operating mode HAND Display Setting range Operating function Temperature set point 0.0 °C to 50.0 °C Must be at least 7 °C / 12.6°F above the ambient temperature in order to ensure a stable temperature inside the C 150. set point 0.0 vol.-% to 20.0 Common value: 5.0 vol.-% (factory setting), or depending on the NaHCO...
Operating mode USER: Advanced settings • In Normal display, press “MODE” and “EXIT” simultaneously for 3 seconds to access the options for selecting the C 150's operating modes. Figure 21: Selecting the operating mode • Press “▲”until the value “OP.U” (operating mode USER) appears in the upper display. •...
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Operating functions in operating mode USER Display Setting range Operating function Temperature set point 0.0 °C to 50.0 °C Must be at least 7 °C / 12.6 °F above the ambient temperature in order to ensure a stable temperature inside the C 150. set point 0.0 vol.-% to 20.0 Common value: 5.0 vol.-% (factory setting), or depending on the NaHCO...
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Display Setting range Operating function Safety controller set point when set point type is “Limit” (Lit) Limit value, i.e., maximum permitted absolute temperature value. When 0.0 °C to 60.0 °C exceeded, the safety controller triggers an alarm. For technical reasons, the limit value should exceed the temperature set point by at least 2 °C.
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Display Setting range Operating function Firmware revision of safety controller for service / maintenance pur- poses. Data record (year) for service / maintenance purposes. Data record (month) for service / maintenance purposes. Data record (day) for service / maintenance purposes. Data set version for service / maintenance purposes.
Operating mode LOCK: Locking/unlocking of the operating functions’ set- tings by operating mode HAND To avoid operating functions being changed by unauthorized persons, you can lock the operating func- tions’ settings. • In operating mode HAND, define a 3-digit numeric password in operating function “PA.H”. Locking the operating functions of operating mode HAND: •...
• Enter the password using the “▲” and “▼”buttons. • Confirm the entry with “MODE”. The controller returns to Normal display. If you have selected any password other than zero, operating mode HAND is temporarily unlocked. Changing the operating functions in operating mode HAND is now possible until a period of 30 sec. has passed with no activity.
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Toggle to operating mode USER Operating mode HAND Operating mode USER If operating mode HAND appears, toggle to operating mode USER with the arrow keys. Password request Password entry (factory setting: “1”). Entry of altitude Entry of the current altitude [km] above sea level with arrow keys. After 30 seconds the controller reverts to Normal Display automatically.
Entering the set points of temperature and CO Normal Display Entry of temperature set point Entry of temperature set point with arrow keys. Entry of CO set point Entry of CO set point with arrow keys. After 30 seconds the controller reverts to Normal Display automatically. When setting a lower temperature set point, in order to save time, we recommend cooling down the unit by turning it off and opening both doors of the unit.
If one of the over temperature protective devices permanently turns off the unit, the user cannot restart the device again. The protective cut-off devices are located internally. Only a service specialist can re- place them. Therefore, please contact an authorized service provider or BINDER Service. Safety controller (temperature safety device class 3.1) The incubator is equipped with an over temperature safety device class 3.1 acc.
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Normal Display simultaneously for 3 seconds Toggle to operating mode USER Operating mode HAND Operating mode USER If operating mode HAND appears, toggle to operating mode USER with the arrow keys. Password request Password entry (factory setting: “1”). 12 x Selecting the set point type Selecting set point type “Limit”...
Select the desired set point type. After 30 seconds the controller reverts to Normal Display automatically. 9.2.2 Setting the safety controller set point You can check and set the safety controller set point in operating modes HAND (chap. 7.2) or USER (chap.
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Password request Password entry (factory setting: “1”). 10 x Depending on the selected set point type (chap. 9.2.1) a different display appears: Entry of limit value or offset value With set point type Offset: With set point type Limit: Enter a limit value Enter an offset value Entry of limit value using the arrow keys.
Alarm functions 10.1 Alarm functions overview When operational faults occur, the controller triggers visual and audible alarm signals. The LED “ALARM” always flashes when an alarm signal is emitted. A zero-voltage relay alarm output (13) (chap. 10.3) permits transmission of the alarm e.g., to a central monitoring system.
10.3 Zero-voltage relay alarm output Collective alarm output via the zero-voltage relay alarm contact The CO incubator is equipped at the rear with a zero-voltage relay output (13) for the temperature and , which permits the transmission of alarms to an external monitoring system in order to monitor and record the alarm signals.
C 150 has cooled down. • If points 1 to 4 do not reveal the source of the fault, it may be that the unit is faulty. Please contact BINDER Service. 10.5 Temperature tolerance range alarm (high and low temperature) Main controller temperature alarm: The temperature has risen above or fallen below the temperature alarm threshold.
Turn on the unit again. You can restart normal operation as soon as the requested values have equilibrated. If the same alarm recurs, please contact BINDER Service. 10.6 Door open The open and closed position of the unit door is controlled via the door contact switch. If the door is open, the temperature and CO controls turn off.
• Open both unit doors for approx. 30 seconds. • Normal operation can be restarted, as soon as the requested values have been readjusted. If the same alarm recurs, please contact BINDER Service. 10.8 CO pressure too low The CO primary pressure at the intake valve is less than 0.3 bar / 4.4 psi below the ambient air pressure.
• Switching the zero-voltage relay alarm output Actions: • Turn off the C 150. • If necessary, clean and disinfect the C 150. Automatic sterilization is not possible with this fault. • Please contact BINDER Service. C 150 (E2) 06/2011 page 48/78...
• Replace the power plug. • Turn the C 150 on with the main power switch. • If the fault shows again, the unit could be faulty. Please contact BINDER Service. Repair must only be performed by qualified service personnel authorized by BINDER.
You need only use an accurate pH indicator or a pH-measuring electrode, which are standard equipment in cell culture laboratories. This method is not suitable for calibrating the BINDER FPI sensor system. This method is based on the acid base equilibrium of the buffer system in the culture media.
All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10% of the full-scale value. These test systems are not suitable for calibrating the BINDER FPI sensor system. Figure 28: Example of chemical indicator...
CO incubators. The CTM 01 is suitable both for reference measurements in certified laboratories, and for service purposes. Please contact the BINDER INDIVIDUAL team. 12.2 Temperature reference measurement When performing a temperature reference measurement using an electronic measuring, and temperature display device, it is important to use a device traceable to an acknowledged standards/calibration institu- tion (DKD, PTB for Germany) with valid calibration certificate.
This could happen e.g. while opening or closing the door, cleaning, loading and unloading the unit. BINDER offers a stacking adapter for direct thermal de- coupled stacking of two incubators.
Avoiding microbial contamination The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, my- coplasma, and viruses. This chapter gives an overview of potential sources of contamination and precau- tions and measures to eliminate them. 14.1 Cells and media •...
• Condensation in the inner chamber represents a particular risk of contamination. The humidifying system with water pan with integrated condensation point developed by BINDER is an effective and easy way to ensure high humidity (95 ± 2% r.H.) inside the incubator without any condensation forming on the inner surfaces.
14.5 Handling the CO incubator C 150 Any manipulation of the CO incubator involves some contamination risks, from installation to opening of the doors and regular cleaning. Installation away from sources of contamination • Do not place the CO incubator on the floor or close to windows and doors. Use the optional stand, if appropriate.
Cleaning, decontamination / disinfection, and sterilization DANGER Electrical hazard. Danger of death. ∅ Do NOT spill water or cleaning agents over the inner and outer surfaces. Disconnect the unit before cleaning. Disconnect the power plug. Completely dry the appliance before turning it on again. 15.1 Cleaning Disconnect the CO incubator from the power supply before cleaning.
For chemical disinfection, we recommend the disinfectant spray Art. No. 1002-0022. Any corrosive damage that may arise following use of other cleaning agents is excluded from liability by BINDER GmbH. 15.2 Decontamination / chemical disinfection Disconnect the CO incubator from the power supply prior to decontamination / disinfection.
15.3 Disinfection of the CO sensor To ensure complete disinfection and proper function of the sensor, BINDER recommends a wipe disinfec- tion of the sensor head with pure alcohol or non-corrosive alcohol-based surface disinfectants. The disin- fectant must be non-corrosive and free of chlorine or any acid. We recommend using the disinfectant Art.
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• Sterilization phase: The temperature is maintained for 3 hours, which ensures that 180 °C / 356 °F is reached on all internal surfaces for at least 30 minutes. • A cooling-down phase follows until 37 °C / 98.6 °F is reached. The precise duration of the entire sterilization cycle depends on the ambient temperature at the installa- tion site and can thus vary.
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• Activate the sterilization procedure: Press the controller button “180 °C” for 3 seconds to access the sterilization program. Figure 32: Initial sterilization display No sterilization can be started if the CO sensor is still plugged-in. You cannot start the sterilization if the operating mode HAND is locked.
CAUTION The glass door and inner chamber become hot during sterilization. Danger of burning. ∅ Do NOT touch the glass door and inner surfaces during sterilization. CAUTION Interruption of the temperature reaction time. Ineffective sterilization. ∅ Do NOT open the unit doors during sterilization. •...
CAUTION Glass door and inner chamber become hot during sterilization. Danger of burning. ∅ Do NOT touch the glass door and inner surfaces for approx. 4 hours after aborting ster- ilization. Aborting sterilization after less than 6 hours When aborting the sterilization prematurely, it may be that the cells/pathogens inside the unit have not all been killed.
• After a manual abortion, the C 150 reverts to its standard operational status. The indication “ - - - “ in the lower display shows that the CO sensor is disconnected. As long as the interior temperature remains above the temperature set to trigger the temperature alarm, the C 150 will trigger alarm signals.
Service personnel authorized by BINDER will check this filter for pollution at each maintenance interval and replace it, if appropriate, but at least once a year.
16.4 Sending the unit back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us.
(Elektro- und Elektronikgerätegesetz, ElektroG) from 23 March 2005, BGBl. I p. 762 or contact BINDER service who will organize taking back and disposal of the unit according to the German national law for electrical and electronic equipment (Elektro- und Elektronik- gerätegesetz, ElektroG) from 23 March 2005, BGBl.
Instruct BINDER Service to dispose of the device. The general terms of payment and delivery of BINDER GmbH apply, which were valid at the time of purchasing the unit. Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to directive 2002/96/EC.
If your distributor is not able to take back and dispose of the unit, please contact BINDER Service. Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to directive 2002/96/EC. The devices must be free from toxic, infectious or radioactive sub- stances in order to eliminate any health hazards to the employees of the recycling companies.
December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals.
18.4 C 150 technical data Exterior dimensions Width mm / inch 680 / 26.77 Height (incl. feet) mm / inch 819/ 32.24 Depth mm / inch 815 / 32.09 Depth plus door handle, I-triangle mm / inch 54 / 2.13 Depth plus power supply connection and gas connec- mm / inch 60 / 2.36...
77°F and a power supply voltage fluctuation of ±10. The temperature data is determined in accordance to BINDER factory standard following DIN 12880, observing the recommended wall clearances of 10% of the height, width and depth of the inner chamber.
18.7 Equipment and options C 150 To operate the CO incubator, use only original BINDER accessories or accessories / compo- nents from third-party suppliers authorized by BINDER. The user is responsible for any risk arising from using unauthorized accessories. Regular equipment...
18.8 Accessories and spare parts BINDER GmbH is responsible for the safety features of the unit only, provided skilled electri- cians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
Contamination clearance certificate Unbedenklichkeitsbescheinigung Declaration regarding safety and health Erklärung zur Sicherheit and gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed.
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Kind of transport / transporter / Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) __________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ C 150 (E2) 06/2011 page 77/78...
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We are aware that, in accordance with Article 823 of the German Civil Code (BGB), we are directly liable with regard to third parties, in this instance especially the employees of BINDER GmbH, who have been entrusted with the handling / repair of the unit / component. / Es ist uns bekannt, dass wir gegenüber Dritten –...
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