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Operating Manual
CB-S / CB-S-UL (E7)
CO
Incubator
2
with FPI-sensor system and RD4 controller
Model
Model version
CB-S 170
CBS170-230V
CB-S 170-UL CBSUL170-120V
CB-S 260
CBS260-230V
CB-S 260-UL CBSUL260-120V
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: +49 7462 2005 0
Fax: +49 7462 2005 100 Internet: http://www.binder-world.com
E-mail: info@binder-world.com Service Hotline: +49 7462 2005 555
Service Fax: +49 7462 2005 93 555 Service E-Mail: service@binder-world.com
Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3
Service Hotline Asia Pacific: +852 390 705 04 or +852 390 705 03
Service Hotline Russia and CIS: +7 495 988 15 16
Issue 06/2020
Art. No.
9040-0189, 9140-0189
9040-0190, 9140-0190
9040-0191, 9140-0191
9040-0192, 9140-0192
Art. No. 7001-0402
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Summary of Contents for Binder CB-S 170
- Page 1 Fax: +49 7462 2005 100 Internet: http://www.binder-world.com E-mail: info@binder-world.com Service Hotline: +49 7462 2005 555 Service Fax: +49 7462 2005 93 555 Service E-Mail: service@binder-world.com Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3 ...
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Page 2: Table Of Contents
Contents SAFETY ........................6 Personnel Qualification ........................6 Operating manual ..........................6 Legal considerations ........................... 6 Structure of the safety instructions ...................... 7 1.4.1 Signal word panel ........................7 1.4.2 Safety alert symbol ........................7 1.4.3 Pictograms ..........................8 1.4.4 Word message panel structure .................... - Page 3 14. CHAMBER SETTINGS (FOR EXPERIENCED USERS ONLY) ......54 14.1 Setting the humidity control ....................... 54 14.2 Setting the door heating offset ......................54 14.3 Adjusting the heating power – for BINDER Service only ..............55 15. NOTIFICATION AND ALARM FUNCTIONS ............55 15.1 Alarm messages ..........................55 15.2 Information messages ........................
- Page 4 16. ETHERNET NETWORK SETTINGS ..............65 16.1 Showing the network settings ......................65 16.1.1 Showing the chamber‘s MAC address ..................65 16.1.2 Showing the IP address ......................65 16.1.3 Showing the subnet mask ....................... 66 16.1.4 Showing the standard gateway ....................66 16.1.5 Showing the DNS server address ...................
- Page 5 24.5.4 Humidity ..........................98 24.5.5 Controller ..........................98 24.5.6 Sterilization ..........................98 24.6 Sending the chamber back to BINDER GmbH ................. 99 25. DISPOSAL......................100 25.1 Disposal of the transport packing ....................100 25.1.1 Outer chamber packing......................100 25.1.2 Packing inside the chamber, equipment ................100 25.2 Decommissioning ..........................
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Page 6: Safety
Operating Manual. This Operating Manual is supplemented and updated as needed. Always use the most recent version of the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to- dateness and validity of this Operating Manual. -
Page 7: Structure Of The Safety Instructions
In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual. This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly, e.g. -
Page 8: Pictograms
1.4.3 Pictograms Warning signs Stability hazard Electrical hazard Hot surface Explosive Atmosphere Gas cylinders Lifting hazard suffocation and Pollution Hazard poisoning hazard Harmful substances Biohazard Risk of corrosion and / or chemical burns Mandatory action signs Disconnect the power Lift with several persons Mandatory regulation Read operating plug... -
Page 9: Localization / Position Of Safety Labels At The Chamber
Figure 2: Position of labels on the CO incubator CB-S on the CO incubator CB-S-UL Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER Service for these replacements. CB-S / CB-S-UL (E7) 06/2020 page 9/119... -
Page 10: Type Plate
CB-S 170 Serial No. 00000000000000 Im Mittleren Ösch 5 78532 Tuttlingen / Germany Made in Germany www.binder-world.com Figure 4: Type plate (example CB-S 170 standard chamber, 9040-0189) Indications of the type plate Information (example) BINDER Manufacturer: BINDER GmbH CB-S 170... -
Page 11: General Safety Instructions On Installing And Operating The Chamber
(for Germany: DGUV guidelines 213-850 on safe working in laboratories, issued by the employers’ liability insurance association). BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relat- ing to chamber safety are replaced in the event of failure with original spare parts. - Page 12 Familiarize yourself with any potential health risks caused by the charging material, the contained mois- ture constituent or by reaction products that may arise during the temperature process. Take adequate measures to exclude such risks prior to putting the CO incubator into operation.
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Page 13: Precautions When Working With Co Gas
Precautions when working with CO Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO warning system. -
Page 14: Intended Use
Such ingredients include in particular acids and halides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH. The chamber does not dispose of any measures of explosion protection. -
Page 15: Foreseeable Misuse
The requirements described in the Operating Manual for installation site and ambient conditions (Chap. 3.4) must be met. WARNING: If customer should use a BINDER chamber running in non-supervised continu- ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible. -
Page 16: Residual Risks
• Non-observance of messages regarding cleaning and disinfection of the chamber. • Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation, cleaning or maintenance. • Cleaning activity while chamber is turned on. • Operation of the chamber with a damaged housing or damaged power cord. •... -
Page 17: Operating Instructions
• Absence of a plausibility check to rule out erroneous inscription of electrical components • Performance of repair work by untrained/insufficiently qualified personnel • Inappropriate repairs which do not meet the quality standard specified by BINDER • Use of replacement parts other than BINDER original replacement parts •... -
Page 18: Measures To Prevent Accidents
1.14 Measures to prevent accidents The manufacturer took the following measures to prevent dangers: • Indications on the type plate See operating manual chap. 1.6. • Operating manual An operating manual is available for each chamber. • Overtemperature monitoring The chamber is equipped with a temperature display, which can be read from outside. The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc. -
Page 19: Chamber Description
A highly precise, drift-free CO infrared measuring system in combination with the perma- nent mixture of CO gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO concentrations for long periods. This creates optimum growth conditions for cultures. -
Page 20: Chamber Overview
The chamber is regularly equipped with an Ethernet interface for computer communication, enabling monitoring via a network. The BINDER APT-COM™ 4 Multi Management Software (option, chap. 20.4) permits networking of up to 100 chambers and connection to a computer, as well as recording and repre- senting temperature and CO data. -
Page 21: Inner Chamber
Inner chamber (11) (10) Figure 6: CO incubator CB-S / CB-S-UL (example CB-S 170), outer door open Triangle instrument panel with RD4 controller for temperature and CO Glass door handle Water pan Shelves (not used) Pt 100 temperature sensor sensor... -
Page 22: Connection Panel On The Rear Of The Chamber
(12b) Socket for IEC connector plug / power cable 100-120 V AC for C-UL (13) DIN-socket for zero-voltage relay alarm outputs (14) DIN socket for analog outputs 4-20 mA (available by BINDER INDIVUDUAL Customized Solutions) (15) Ethernet interface for computer communication (16a) Miniature fuse T10 A (L) 250 V AC for C... -
Page 23: Chamber Doors
Chamber doors The outer chamber door is equipped with a heater on its inner side. The door must be closed while the chamber is operating normally in order to ensure stable climatic conditions in the inner chamber. An additional glass door enables viewing of the samples without disturbing the temperature in the interior and contaminating the samples sealing the interior of the chamber. -
Page 24: Completeness Of Delivery, Transportation, Storage, And Installation
Note on second-hand chambers (Ex-Demo-Units): Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber. -
Page 25: Guidelines For Safe Lifting And Transportation
• Permissible ambient temperature range for transport: -10 °C / 14°F to +60 °C / 140°F. You can order transport packing and rolling pallets for transportation purposes from BINDER Service. Storage Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for temporary decommissioning (chap. - Page 26 NOTICE Danger of overheating due to lack of ventilation. Damage to the chamber. ∅ Do NOT install the chamber in unventilated recesses. Ensure sufficient ventilation for dispersal of the heat. Observe the prescribed minimum distances when installing the chamber. Do not install or operate the chamber in potentially explosive areas.
- Page 27 Notes on handling carbon dioxide (CO Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO warning system.
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Page 28: Installation And Connections
Installation and connections Shelves You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the shelf straight and then insert it so it will go smoothly inside the chamber. Permitted shelf loads: Maximum load on one single shelf: 10 kg / 22 lb Maximum total load on all shelves:... -
Page 29: Water Pan
The CO sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F. NOTICE Danger of damage to the CO sensor by excess temperature. Damage to the CO sensor. ∅ Do NOT autoclave the CO Sensor. ∅... -
Page 30: Gas Connection
Make sure that the water pan has firm contact with the inner chamber bottom and rests tightly on it. Fill the water pan with 1 liter of distilled, sterilized water. We recommend cleaning (chap. 22.1) and refilling the pans 2 to 3 times a week. For evacuation, remove the water pan. -
Page 31: Connecting The Co Gas Cylinder
4.4.1 Connecting the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO warning system. - Page 32 Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to con- nect the original hose nozzle. In this case, please contact BINDER Service. Remove the rubber cover (b) by pulling it off.
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Page 33: Gas Cylinder Connection Kit (Option)
4.4.3 Gas cylinder connection kit (option) The gas cylinder connection kit for CO (Art. no. 8012-0014) includes the following parts for connecting a gas cylinder to the CO incubator: • Pressure reducer with manometers for cylinder pressure (high pressure gauge) and outlet pressure (low pressure gauge) •... -
Page 34: Electrical Connection
• Only use original connection cables from BINDER according to the above specification. • Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located on the chamber’s type plate (centrally located at the bottom of the left-hand side of the... -
Page 35: Functional Overview Of The Rd4 Chamber Controller
“Set points” menu directly at the controller or use the APT-COM™ 4 Multi Management Software (option) specially developed by BINDER. The controller offers various notifications and alarm messages with visual and audible indication. All con- troller setting remain valid until the next manual change. They are stored also after turning off the cham- ber. -
Page 36: Menu Structure Of The Controller And Access Levels
• Admin: The password enables access to advanced controller functions and settings. Factory setting is 00 01. • Service: The password enables access to all controller functions (for BINDER Service only). As soon as a password has been assigned, access to the respective functions is blocked and only avail- able after entering the correct password. -
Page 37: Performance During And After Power Failures And Shut Down
(chap. 22). WARNING: If customer should use a BINDER chamber running in non-supervised continu- ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible. -
Page 38: Preset Factory Parameters
1 min chap. 13.4 Password for “User” access level. chap. 10.2.1 Password for “Admin” access level. chap. 10.2.2 The following settings should only be modified by BINDER service: Humidity control 0 % r.h. chap. 14.1 Door heating Offset value 3.0 °C chap. -
Page 39: Altitude Of The Installation Site Above Sea Level
• Current time (chap. 12.4), format HH:MM: Use the arrow buttons to set the hours, continue with the OK button. Use the arrow buttons to set the minutes, confirm with the OK button Set the controller to the desired temperature, and CO set points that shall be used to operate the cham- ber (chap. -
Page 40: Temperature And Co Set-Point Entry
Temperature and CO set-point entry Required access level: “User”. Setting ranges Control ranges Temperature 0 °C / 32 °F up to 50 °C / 122 °F 6 °C / 10.8 °F above ambient up to 50 °C / 122 °F Humidity 0 vol.-% up to 20 vol.-% 0 vol.-% up to 20 vol.-%... -
Page 41: Placing Samples In The Incubator
If no CO concentration is indicated (display showing “ – – – – ”) the CO sensor is not con- nected. Note when setting high gas concentrations Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. -
Page 42: Setting Special Controller Functions
Setting special controller functions In the “Functions on/off” menu you can define the switching state of the special controller functions. Required access level: “User”. Path: Normal display Setpoints Functions on/off The functions are displayed from left to right. Example: Function 1 “Humidity off” activated = 1000. Function 1 “Humidity off” deactivated = 0000. Submenu “Functions on/off”. -
Page 43: Deactivated Co 2 Control
Deactivated CO control When operating the chamber without a CO2 gas connection, you can deactivate CO2 control with the function “CO2 off” (chap. 9) to avoid alarms of the CO gas system. No CO tolerance range alarms and no pressure alarm will be emitted. The actual CO value continues to be displayed in Normal Display. -
Page 44: Assign And Modify A Password
10.2 Assign and modify a password In this menu you can assign and modify the passwords of the “User” and “Admin” access levels. Required access level: “Admin”. Keep the password well in mind. There is no access to the corresponding menu functions without the correct password. -
Page 45: Temperature Safety Devices
If one of the over temperature protective devices permanently turns off the chamber, the user cannot restart the device again. The protective cut-off devices are located internally. Only a service specialist can replace them. Therefore, please contact an authorized service provider or BINDER Service. 11.2 Overtemperature safety controller class 3.1 The chambers are regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880:2007). -
Page 46: Setting The Safety Controller Mode
11.2.1 Setting the safety controller mode Required access level: “User”. Path: Normal display Setpoints Safety controller Mode Press the OK button to enable the setting. Setting the safety controller mode The current setting flashes. Use the arrow buttons to select between LIMI (Limit) and OFFS (Offset). -
Page 47: Message And Measures In The State Of Alarm
11.2.3 Message and measures in the state of alarm The state of alarm is indicated visually in Normal display. If the buzzer is enabled (chap. 15.3) there is an additional audible alert. The heating turns off. As soon as the inner chamber temperature has cooled down below the safety controller value, the heating is released and temperature control continues. -
Page 48: General Controller Settings
General controller settings The general settings can be accessed in the “Settings” submenu, which is available for users with “Ser- vice” or “Admin” authorization level. It serves to enter date and time, select the language for the controller menus and the desired temperature unit and to configure the controller’s communication functions. The display of some network settings is available for all users in the “Chamber... -
Page 49: Setting The Current Date
12.3 Setting the current date Required access level: “Admin”. Following start-up of the chamber (chap. 6.3), it is “User”. Path: Normal display Settings Chamber Date Press the OK button to enable the setting. Setting the date: day The current setting flashes. Enter the current day with the arrow but- tons. -
Page 50: Setting The Current Time
12.4 Setting the current time Required access level: “Admin”. Following start-up of the chamber (chap. 6.3), it is “User”. Path: Normal display Settings Chamber Time Press the OK button to enable the setting. Setting the time: hours The current setting flashes. Enter the current hour with the arrow but- tons. -
Page 51: Setting The Chamber Address
12.6 Setting the chamber address This setting is required for the communication with the BINDER APT-COM™ 4 Multi Management Soft- ware. The chamber address settings in the chamber controller and in the software must be identical. Required access level: “Admin”. -
Page 52: Tolerance Range Settings
Tolerance range settings In this menu you can define for temperature and CO the deviation between the actual value and setpoint, which that shall cause a tolerance range alarm. The entered value defines the limit of permitted devia- tions from the set-point (exceeding and falling below). Reaching this limit triggers tolerance alarm. In addition you can specify a delay time for this alarm. -
Page 53: Setting The Co Tolerance Range
13.3 Setting the CO tolerance range Path: Normal display Settings Various CO2 range Press the OK button to enable the setting. Setting the CO tolerance range The current setting flashes. Enter the desired CO range with the ar- row buttons. Entry range: 1.0 vol.-% up to 10.0 vol.-% Confirm the entry with the OK button. -
Page 54: Chamber Settings (For Experienced Users Only)
If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance. Path: Normal display... -
Page 55: Adjusting The Heating Power - For Binder Service Only
14.3 Adjusting the heating power – for BINDER Service only Depending on the duration of the door opening, a heating power factor for humidity recovery time can be adapted. Factory setting: 12.0. This setting is intended for BINDER Service only! - Page 56 Alarm messages overview: Moment of alarm message and Condition Icon Alarm message switching the zero-voltage alarm relay output after configurable time Chamber door open “Door open” (chap. 13.4). Factory setting: 1 minute Setpoint of the safety controller “Safety controller” immediately class 3.1 exceeded Tolerance range alarm: actual after configurable time...
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Page 57: Information Messages
15.2 Information messages Information messages provide information about the settings made or the current controller condition. In Normal display a text message indicates the condition. The “Information” icon is lit. Information message (example: sterilization running) Sterilization Information messages overview: Moment of information Condition Icon Information message... -
Page 58: Activating / Deactivating The Audible Alarm (Alarm Buzzer)
Nor- display. 15.4 Measures in case of alarm Only qualified service personnel authorized by BINDER must perform repair. Repaired chambers must comply with the BINDER quality standards. 15.4.1 Door open alarm The open and closed position of the chamber door is controlled via the door contact switch. If the door is open, the temperature and CO controls turn off. -
Page 59: Temperature Tolerance Range Alarm (Overtemperature / Too Low Temperature)
• To decrease the temperature, proceed as follows: Turn off the chamber. Open both chamber doors for approx. 5 minutes. Turn on the chamber again. You can restart normal operation as soon as the re- quested values have equilibrated. If the same alarm recurs, please contact BINDER Service. CB-S / CB-S-UL (E7) 06/2020 page 59/119... -
Page 60: Co Tolerance Range Alarm (Co Over/Under Concentration)
• Open both chamber doors for approx. 30 seconds. Respect the precautions when working with CO gas (chap. 1.8). • Normal operation can be restarted, as soon as the requested values have been readjusted. • Reset the alarm message. If the same alarm recurs, please contact BINDER Service. 15.4.5 CO pressure alarm If CO control has been deactivated (chap. -
Page 61: Power Failure Alarm
• If these points do not reveal the source of the fault, it may be that the chamber is faulty. Please contact BINDER Service. The outlet pressure of the gas cylinder must be 2.0 bar / 29 psi above the ambient pressure. -
Page 62: Alarms Referring To Temperature Sensor Failures
• Turn off the chamber. • If necessary, clean and disinfect the chamber. Automatic sterilization is not possible with this fault. • Please contact BINDER Service. Failure of inner temperature sensor • Actual temperature value display shows “ – – – – ”... -
Page 63: Alarm On Ineffective Sterilization
• Turn off the chamber. • Remove the sensor (chap. 4.2), replace it if necessary. • If necessary, clean, disinfect and sterilize the chamber before restarting it • Please contact BINDER Service. sensor not connected • Actual CO value display shows “ – – – – ”... -
Page 64: Zero-Voltage Relay Alarm Output
15.5 Zero-voltage relay alarm output Collective alarm output via the zero-voltage relay alarm contact The chamber is equipped at the rear with a zero-voltage relay output (13) for temperature and CO , which permits the transmission of alarms to an external monitoring system in order to monitor and record the alarm signals. -
Page 65: Ethernet Network Settings
Ethernet network settings The settings of the “Ethernet” submenu are used for networking chambers with an Ethernet interface, e.g. to connect them with BINDER’s APT-COM™ 4 Multi Management Software (option, chap. 20.4). 16.1 Showing the network settings Required access level: “User”. -
Page 66: Showing The Subnet Mask
16.1.3 Showing the subnet mask Path: Normal display Chamber info Ethernet Subnet mask Display of the subnet mask (exam- ple) Toggle forth and back with the Back button and the OK button. Subnet mask 0.0.0.0 With the arrow-down button you can now change to the next parameter (standard gateway). With the Back button you can go back to the “Ethernet”... -
Page 67: Showing The Dns Chamber Name
16.1.6 Showing the DNS chamber name Path: Normal display Chamber info Ethernet chamber name Display of the DNS chamber name (example) Toggle forth and back with the Back button and the OK button. DNS chamber name BinderRD4-000cd80a281 With the Back button you can go back to the “Ethernet” submenu and, repeatedly pressing it, to Normal display. -
Page 68: Selecting The Type Of Assignment Of The Dns Server Address (Automatic / Manual)
16.2.2 Selecting the type of assignment of the DNS server address (automatic / manual) Access to this function is possible only if automatic IP address assignment has been selected (chap. 16.2.1). Path: Normal display Settings Ethernet DNS server Press the OK button to enable the setting. Selection of the type of assignment of the DNS server address. -
Page 69: Setting The Subnet Mask
IP address assignment (sample values). The forth section of the IP address is shown. Enter the desired value with the arrow buttons. Confirm the setting with the OK button. IP address With the arrow-down button you can now change to the enter the subnet mask. With the Back button you can go back to the “Ethernet”... -
Page 70: Assigning The Dns Server Address
• Chamber data for BINDER Service “DL2” This data is intended for use by BINDER Service. It also contains information from the self-test func- tion. The storage rate is fix (1 minute). Temperature values are always given out in °C. -
Page 71: Storage Capacity
(chap. 18.2) and when loading a new firmware version by BINDER service, the entire data memory is deleted. BINDER service can also install the configuration by means of a setup program without deleting the data. Regardless of this, BINDER Service can delete the data via a setup program. -
Page 72: Usb Menu: Data Transfer Via Usb Interface
For detailed information on the file content see chap. 17.1. • Service data The "Service" folder is created on the USB stick and can be sent to BINDER Service. In addition to the configuration and recorder data, it contains further service-relevant information. -
Page 73: Import Function
18.2 Import function Required access level: “Admin”. Function “Import configuration”. To import configuration data from the USB stick, press the OK button. Import configuration With the arrow-down button you can now change to the setting of the “Export configuration” function. 18.3 Export functions Required access level: any user Function “Export configuration”. -
Page 74: Ongoing Data Transfer
18.4 Ongoing data transfer A moving arrow symbol indicates the progress of the data transfer. Example: Data recording is running. Export service data Attention! Danger of data loss! Do not disconnect the USB stick from the device during ongoing data transfer! After successful transfer, the controller shows again the initial function “Import configuration”. -
Page 75: Reference Measurements
You need only use an accurate pH indicator or a pH-measuring electrode, which are standard equipment in cell culture laboratories. This method is not suitable for calibrating the BINDER FPI sensor system. This method is based on the acid base equilibrium of the buffer system in the culture media. -
Page 76: Measuring Co Directly Via Chemical Indicator Tubes
All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10% of the full-scale value. These test systems are not suitable for calibrating the BINDER FPI sensor system. Figure 17: Example of chemical indicator... -
Page 77: Temperature Reference Measurement
CO incubators. The CTM 01 is suitable both for reference measurements in certified laboratories, and for service purposes. Please contact the BINDER INDIVIDUAL team. 19.2 Temperature reference measurement When performing a temperature reference measurement using an electronic measuring, and temperature display device, it is important to use a device traceable to an acknowledged standards/calibration institu- tion (DKD, PTB for Germany) with valid calibration certificate. -
Page 78: Base On Castors (Option)
This could happen e.g. while opening or closing the door, cleaning, loading and unloading the chamber. BINDER offers a flat stacking frame for safe stacking of two incubators. -
Page 79: Analog Outputs For Temperature And Co (Option)
20.6 Analog outputs for temperature and CO (option) With this option, the CO incubator is equipped with analog outputs 4-20 mA for temperature and CO These outputs allow transmitting data to external data registration systems or devices. The connection is realized as a DIN socket at the rear of the CO incubator as follows: ANALOG OUTPUT 4-20 mA DC PIN 1: Temperature –... -
Page 80: Working And Behavior In The Lab
• Use air conditioning with special filters. • Reduce the number of personnel and their movements in the lab by careful positioning all relevant equipment. For practical reasons, install the CO incubator close to the laminar air flow bench. • Regular microbiological monitoring of the cell culture laboratory. 21.3 Working and behavior in the lab Sources of contamination are often the laboratory personnel themselves (surface germs, oral flora droplet transfer) and handling the equipment and cultures. -
Page 81: Handling The Co Incubator
• Condensation in the inner chamber represents a particular risk of contamination. The humidifying sys- tem with water pan with integrated condensation point developed by BINDER is an effective and easy way to ensure high humidity (95 +/- 2% r.h.) inside the incubator without any condensation forming on the inner surfaces. -
Page 82: Cleaning, Decontamination / Disinfection, And Sterilization
• Replace the CO sterile filter (once or twice a year depending on usage). What to do in case of contamination? • Throw away / autoclave contaminated cultures. • Carefully inspect cultures that seem to be uncontaminated. • Clean the incubator as described. Wipe the inner chamber and the doors with a disinfectant and allow to dry. -
Page 83: Cleaning
We recommend using the neutral cleaning agent Art. No. 1002-0016 for a thorough cleaning. Any corrosive damage that may arise following use of other cleaning agents is excluded from liability by BINDER GmbH. Use only the products recommended by BINDER for cleaning and disinfection NOTICE Danger of corrosion by using unsuitable cleaners. -
Page 84: Decontamination / Chemical Disinfection Of The Chamber
For chemical disinfection, we recommend the disinfectant spray Art. No. 1002-0022. Any corrosive damage that may arise following use of other disinfectants is excluded from liability by BINDER GmbH. With every decontamination / disinfection method, always use adequate personal safety con- trols. -
Page 85: Disinfection Of The Co
22.3 Disinfection of the CO sensor To ensure complete disinfection and proper function of the sensor, BINDER recommends a wipe disinfec- tion of the sensor head with pure alcohol or non-corrosive alcohol-based surface disinfectants. The disin- fectant must be non-corrosive and free of chlorine or any acid. We recommend using the disinfectant Art. -
Page 86: Hot-Air Sterilization At 180 °C / 356 °F
We recommend regular disinfection of the CO sensor. NOTICE Danger of damage to the CO sensor when connecting or removing it during opera- tion. Damage to the CO sensor. Connect or remove the CO sensor only with the chamber turned off. Recommended procedure: •... -
Page 87: Performing A Hot-Air Sterilization
The chamber can perform an automatically controlled hot-air sterilization cycle. This procedure will take approx. 12 hours and consists of the following steps: • Heating up phase: The incubator heats up the inner chamber as fast as possible to the sterilization set-point temperature •... -
Page 88: Starting And Running The Hot-Air Sterilization Cycle
• Empty the water pan. WARNING Danger of implosion caused by a filled water pan during sterilization. Damage to the chamber. Empty the water pan before starting the hot-air sterilization. • Clean the chamber. • Water pan and shelves must be inside the incubator, the water pan at its usual place on the bottom. Before starting a hot-air sterilization, the entire interior must be clean and dry. -
Page 89: Performance During Sterilization
23.3.2 Performance during sterilization Normal display during sterilization (example during heating up phase) Sterilization The information message “Sterilization” is shown alternating with the indication “Temp. / CO2”. The CO actual value display shows “ – – – – ” because the CO sensor is disconnected. -
Page 90: Prematurely Terminating The Sterilization Cycle - Effects
23.4 Prematurely terminating the sterilization cycle – effects Prematurely terminating the sterilization cycle means terminating it before the entire 12-hour duration is completed. Whether effective sterilization has occurred depends on the time that has elapsed before. • Premature termination after less than 6 hours: Prevents effective sterilization. This is indicated by the alarm message “Sterilization failed”. -
Page 91: Premature Termination After More Than 6 Hours, I.e., During The Cooling-Down Phase: Successful Sterilization
23.4.2 Premature termination after more than 6 hours, i.e., during the cooling-down phase: successful sterilization The duration of the entire sterilization is approx. 12 hours. If you want to shorten the sterilization proce- dure in order to save time, you can prematurely terminate it during the cooling-down phase, i.e. no sooner than after 6 hours. -
Page 92: Opening The Outer Door
• Do not open the chamber doors until the interior temperature has dropped to 37 °C / 98.6 °F. • After premature termination the chamber reverts to its standard operational status. • The CO actual value display shows “ – – – – ” because the CO sensor is disconnected. -
Page 93: Maintenance, And Service, Troubleshooting, Repair, Testing
For personnel requirements please refer to the Service Manual. • Repair Repair of the chamber can be performed by BINDER Service or by BINDER qualified service partners or technicians, in accordance with the description in the Service Manual. After maintenance, the chamber must be tested prior to resuming operation. -
Page 94: Maintenance Intervals, Service
When exchanging the sensor, you must repeat the CO adjustment. We recommend taking out a maintenance agreement. Please consult BINDER Service. BINDER telephone hotline: +49 (0) 7462 2005 555 BINDER fax hotline:... -
Page 95: Gas Inlet Fine Filter
If there are is a technical fault or shortcoming, take the chamber out of operation and inform BINDER Service. If you are not sure whether there is a technical fault, pro- ceed according to the following list. If you cannot clearly identify an error or there is a technical fault, please contact BINDER Service. -
Page 96: Temperature
No. Fault description Possible cause Required measures Pt 100 sensor defective. Chamber does not heat up. Heating element defective. Contact BINDER service. Semiconductor relay defective. Chamber is heating permanent- Semiconductor relay defective. Contact BINDER service. ly. Set-point is not respected. -
Page 97: Co 2
Turn off the chamber. If appro- “ – – – – ”. Alarm message sensor defective. priate, replace sensor (chap. “CO2 sensor defective” 4.2). Contact BINDER service. Actual CO value display shows Turn off the chamber. Connect sensor not connected, “... -
Page 98: Humidity
Doors not closed. Close door properly. Door gaskets defective. Replace door gaskets. Place the chamber on a Chamber placed on very cold BINDER stand to increase the floor. distance to the floor. Doors not closed. Close door properly. Condensation on the door. -
Page 99: Sending The Chamber Back To Binder Gmbh
24.6 Sending the chamber back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us. -
Page 100: Disposal
Disposal 25.1 Disposal of the transport packing 25.1.1 Outer chamber packing Packing element Material Disposal Straps to fix packing on pallet Plastic Plastic recycling (no image) Shipping box Cardboard Paper recycling Edge stuffing, top PE foam Plastic recycling PE foam Plastic recycling Pallet with foamed plastic stuffing... -
Page 101: Decommissioning
In this case, clean and dry the incubator running at 37 °C / 98.6 °F with doors open for at least one hour before loading it with samples. BINDER recom- mends performing a hot air sterilization of the chamber before commissioning. -
Page 102: Disposal Of The Chamber In The Member States Of The Eu Except For The Federal Republic Of Germany
According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control instruments” (category 9) only intended for professional use“. They must not be disposed of at public col- lecting points. -
Page 103: Disposal Of The Chamber In Non-Member States Of The Eu
NOTICE Danger of violation against existing law if not disposed of properly. Alteration of the environment. For final decommissioning and disposal of the chamber, please contact BINDER ser- vice. Follow the statutory regulations for appropriate, environmentally friendly disposal. -
Page 104: Technical Description
(certified since December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals. -
Page 105: Cb-S / Cb-S-Ul Technical Data
The technical data refers to the so defined usable volume. Do NOT place samples outside this usable volume. Do NOT load this volume by more than half to enable sufficient airflow inside the CO incuba- tor. Do NOT divide the usable volume into separate parts with large area samples. Do NOT place samples too close to each other in order to permit circulation between them and thus obtain a homogenous distribution of temperature and CO 26.4 CB-S / CB-S-UL technical data... -
Page 106: Important Conversion Data For Non-Si Units
+22 °C +/- 3 °C / 71.6 °F +/- 5.4 °F and a power supply voltage fluctuation of +/-10%. The technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN 12880:2007. All indications are average values, typical for chambers produced in series. We reserve the right to change technical specifications at any time. -
Page 107: Conversion Table For Gas Inlet Pressures, Bar - Psi
65.2 26.7 Equipment and options (extract) To operate the chamber, use only original BINDER accessories or accessories / components from third-party suppliers authorized by BINDER. The user is responsible for any risk arising from using unauthorized accessories. Regular equipment Microprocessor controller RD4 for temperature and CO infra-red absorption measuring system Hot-air sterilization at 180 °C / 356°F... -
Page 108: Options, Accessories, And Spare Parts (Extract)
26.8 Options, accessories, and spare parts (extract) BINDER GmbH is responsible for the safety features of the chamber only, provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts. -
Page 109: Dimensions
26.9 Dimensions CB-S / CB-S-UL 170: [Dimensions in mm] CB-S / CB-S-UL (E7) 06/2020 page 109/119... - Page 110 CB-S / CB-S-UL 260: [Dimensions in mm] CB-S / CB-S-UL (E7) 06/2020 page 110/119...
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Page 111: Certificates And Declarations Of Conformity
Certificates and declarations of conformity 27.1 EU Declaration of Conformity CB-S / CB-S-UL (E7) 06/2020 page 111/119... - Page 112 CB-S / CB-S-UL (E7) 06/2020 page 112/119...
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Page 113: Product Registration
Product registration CB-S / CB-S-UL (E7) 06/2020 page 113/119... -
Page 114: Contamination Clearance Certificate
Contamination clearance certificate Unbedenklichkeitsbescheinigung 29.1 For chambers located outside the USA and Canada Declaration regarding safety and health Erklärung zur Sicherheit und gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed. - Page 115 Kind of transport / transporter / Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) __________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ CB-S / CB-S-UL (E7) 06/2020 page 115/119...
- Page 116 We are aware that, in accordance with Article 823 of the German Civil Code (BGB), we are directly liable with regard to third parties, in this instance especially the employees of BINDER GmbH, who have been entrusted with the handling / repair of the unit / component. / Es ist uns bekannt, dass wir gegenüber Dritten –...
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Page 117: For Chambers In The Usa And Canada
Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA number. Please be aware that size specifications, voltage specifications as well as performance specifi- cations are available on the internet at www.binder-world.us... - Page 118 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is com- pleted by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
- Page 119 4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc. for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.
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