Cb (e7), co2 incubators, co2 incubators with o2 control, co2 incubators with active humidification.
with sterilizable ndir sensor system for co2 and microprocessor program controller mb2
4.5.2 Water supply installation ......................37 4.5.3 BINDER Pure Aqua Service (option) ..................37 Electrical connection ......................... 38 Handling and aligning the divided inner door, gas proof (optional equipment) ......... 39 FUNCTIONAL OVERVIEW OF THE MB2 CHAMBER CONTROLLER ....40 Operating functions in normal display ....................
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Principle of controller entries ......................49 Performance during and after power failures ..................49 Performance when opening the door ....................50 START UP ......................50 Turning on the chamber ........................50 Controller settings upon start up ....................... 51 Entering the altitude of the installation site ..................52 Factory settings ..........................
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14.4.1 Adapting the display parameters ..................109 14.4.2 Touchscreen calibration ......................110 14.5 Network and communication ......................111 14.5.1 Serial RS485 interface (available via BINDER INDIVIDUAL Customized Solutions) ..111 14.5.2 Ethernet ..........................112 14.5.2.1 Configuration ......................... 112 14.5.2.2 Display of MAC address ....................113 14.5.3...
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18.1 Communication software APT-COM™ 3 DataControlSystem (option)........... 124 18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ..124 18.3 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) ..... 125 18.4 Analog outputs for temperature and CO (option) ................
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22.2 Checking the humidity system fan ....................145 22.3 Gas inlet fine filter ........................... 145 22.4 Replacing the CO sensor ....................... 145 22.5 Sending the chamber back to BINDER GmbH ................146 23. DISPOSAL ......................147 23.1 Disposal of the transport packing ....................147 23.1.1 Outer chamber packing ......................
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual.
WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury. CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury. CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity.
Mandatory action signs Mandatory regulation Read operating Lift with several persons Disconnect the power instructions plug Wear protective gloves Wear safety goggles Environment protection Prohibition signs Do NOT touch Do NOT spray with Do NOT climb water Information to be observed in order to ensure optimum function of the product. 1.2.4 Word message panel structure Type / cause of hazard.
• on the outer door above the door handle incubator CB incubator CB-UL Figure 1: Position of labels on the chamber Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER service for these replacements. CB (E7) 06/2018 Page 10/174...
CB 170 Serial No. 00000000000000 Im Mittleren Ösch 5 Made in Germany 78532 Tuttlingen / Germany www.binder-world.com Figure 2: Type plate (example of CB 170 regular chamber) Indications of the type plate (example) Information BINDER Manufacturer: BINDER GmbH CB 170...
BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany). BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
During and after a sterilization the temperature of the inner surfaces almost equals the set-point. CAUTION The inner doors, the inner door and glass door handles, and the inner chamber will become hot during a sterilization. Danger of burning. ∅ Do NOT touch the inner doors, the inner door and glass door handles, and the inner surfaces after a sterilization.
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Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible. General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures.
Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store and use gas cylinders only in well-ventilated locations. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). •...
Due to the special demands of the Medical Device Directive (MDD), these chambers are not qualified for sterilization of medical devices as defined by the directive 93/42/EWG. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
Measures to prevent accidents The manufacturer took the following measures to prevent dangers: • Indications on the type plate See operating manual chap. 1.4. • Operating manual An operating manual is available for each chamber. • Overtemperature monitoring The chamber is equipped with a temperature display, which can be read from outside. The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
1.10 Resistance of the humidity sensor against harmful substances The following list of harmful substances refers only to the humidity sensor and does not include any other materials incorporated in the chamber or prohibited substances in relation to explosion protection. Some gases - especially clean gases - do not have any influence on the humidity sensor.
A resistance humidifying system humidifies the air. For this purpose, use deionized (demineralized) water. The option BINDER Pure Aqua Service allows using the chamber with any degree of water hardness. Controller: The efficient program controller is equipped with a multitude of operating functions, in addition to recorder and alarm functions.
Pt 100 temperature probe sensor (chamber with O control) Humidity sensor (chamber with active humidification) Internal socket 230V (max. 3 A) (option, available via BINDER INDIVIDUAL Customized Solutions, chap. 18.3) Perforated shelves, made of stainless steel Glass door handle Measuring access port Inner glass doors Permadry™...
(1b) Socket for IEC connector plug / power cable 230 V AC External socket for extra-low voltage supply (option for CB 170 / CB 220, chap. 18.6) DIN-socket for zero-voltage relay alarm outputs DIN socket for analog outputs 4-20 mA (option, chap. 18.4)
Note on second-hand chambers (Ex-Demo-Units): Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber.
• If the humidifying system has NOT been emptied: +3 °C / 37.4 °F to +60 °C / 140 °F. • After BINDER Service has emptied the humidifying system: -10 °C / 14 °F to +60 °C / 140 °F.
The chambers can be stacked on top of each other (two chambers maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 18.8.1) or the stacking adapter (chap. 18.8.2).
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An example of how to evaluate laboratory volume and air change rate: Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters / 3,531.5 cubic feet with 10 incubators CB 170, opened 4 times per hour? Calculation: CO...
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0.00758 cubic meters x 10 x 4 div. 100 cubic meters = 0.003032, i.e. 0.303 % or 3032 ppm. 0.2676852 cubic feet x 10 x 4 div. 3,531.467 cubic feet = 0.003032, i.e. 0.303 % or 3032 ppm. Result: The maximum permissible value of 5000 ppm is not exceeded under these operation conditions.
Put the Permadry™ water pan on the bottom of the inner chamber in a way that both notches lock into place. • The front side of the CB 170 / CB 220 Permadry™ water pan is marked “FRONT”. Figure 9: Letters “FRONT” indicating the front of the CB 170 / CB 220 Permadry™ water pan...
Fill only the outer pan with distilled, sterilized water up to the filling level marking on the edge of the inner pan. Maximum filling quantity of the outer pan: CB 56: approx. 0.7 liters, CB 170: approx. 1.3 liters, CB 260: approx. 2.0 liters.
Gas connections General information for safe handling of gas cylinders: • Store and use gas cylinders only in well ventilated areas. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). •...
4.4.1 Connection of the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation warning system.
The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 25.4) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times. Conversion table for gas inlet pressures, bar –...
Establishing the connection to the chamber Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp. Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (11) DN 6 on the chamber rear, as described in chap.
Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to connect the original hose nozzle. In this case, please contact BINDER Service. CB (E7) 06/2018...
Remove the rubber cover (b) by pulling it off. Chamber rear Figure 13: Connecting the hose lead to the gas cylinder Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.
1 µS/cm (ultrapure water), may cause acid corrosion due to its low pH). • Tap water that has been treated by the optional water treatment system BINDER Pure Aqua Service (disposable system). A reusable measuring equipment to assess the water quality is included (chap.
4.5.3 BINDER Pure Aqua Service (option) The optional BINDER water treatment system (disposable system) is available to treat tap water. The lifetime depends on water quality and the amount of treated water used. The measuring equipment to assess the water quality is reusable.
Measuring access port Figure 15: Divided inner door, gas proof (example: CB 170) • Pull the handles to open each of the individual glass doors. Do not press too hard while closing them to avoid pushing the doors through the gasket into the inner chamber.
The MB2 program controller permits programming temperature and CO and humidity cycles, and specifying special controller functions for each program section. You can enter values or programs directly at the controller or use the APT-COM™ 3 DataControlSystem software (option) specially developed by BINDER. Operating mode Temperature values...
Operating functions in normal display Current operating mode Text list for information icons Date, time, authorization level of the logged-in user, memory Quick setpoint entry Continue to next screen Back to Normal display Information Program start Setpoint entry Event list Display of active alarms Access to main menu Figure 17: Operating functions of the MB2 controller in normal display (sample values, chamber with O...
Display views: Normal display, program display, chart-recorder display Press the Change view icon to toggle between normal display, program display and chart- recorder display. Press the Normal display icon to return from program display and chart recorder display back to Normal display.
Controller icons overview Navigation icons in Normal display Icon Signification Function Main menu Access from Normal display to the main menu Alarm Access from Normal display to the list of active alarms Event list Access from Normal display to the event list Access from Normal display to the setpoint entry menu: setpoint Setpoint setting entry for Fixed value operation, turning on/off CO...
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Functional icons in the chart recorder display Icon Signification Function Show legend Show legend Hide legend Hide legend Switch legend Switch between legend pages Show indication “Door open” (B2) Show indications Hide indications Hide indication “Door open” (B2) Pause chart recorder and change to history display. Data recording History display continues.
Operating modes The MB2 program controller operates in the following operating modes: • Idle mode The controller is not functional, i.e., there is no heating, no CO inlet or O inlet (O control) and no humidification (active humidification). The chamber approximates ambient values. You can activate and deactivate this operating mode with the “Idle mode”...
Controller menu structure Use the navigation icons in the screen footer in Normal display to access the desired controller functions. The available functions depend on the current authorization level “Service”, “Admin” or “User” (chap. 13.1). This is selected either during login or can be available without password protection. Main menu: program settings, further information, “Service”...
• Access to service data, controller reset to factory settings (chap. 5.5.3) • Available only for users with “Service” and “Admin” authorization level. Full functional range only for BINDER Service (users with “Service” authorization level). “Programs” submenu • Access to the controller’s program functions (chap. 8, 9, 10)
5.5.3 “Service” submenu The “Service” submenu is available for users with “Service” or “Admin” authorization level. When logged- in with “Admin” authorization level the user will find information to tell the BINDER Service in service case. Path: Main menu > Service Serial number of the chamber, setup version chap.
Principle of controller entries In the selection and entry menus there are icons displayed in the footers which you can use to take over the entry or cancel it. Entry menu (example) Selection menu (example) After completing the settings there are the following possibilities: Press the Confirm icon to take over the entries and exit the menu or continue the menu sequence.
To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
Controller settings upon start up The window „Language selection“ enables the language selection, in case that it’s activated in the “Start-up” menu. Afterwards occurs a request of the time zone and the temperature unit. The controller will function in the operating mode, which was active before the last shut-down. It controls temperature, CO (chamber with O control) and humidity (chamber with active humidification) in fixed...
Entering the altitude of the installation site After first turning on the chamber, it is required to enter the altitude above sea level of the installation site into the controller. This entry serves to correct the calculation of CO concentration in vol.-% from the measurement of partial pressure.
Equilibration time Temperature • Equilibration time is max. 4 hours. • After turning in the chamber, after approx. 5 minutes the CO concentration equilibrates automatically to the pre-set value of 5 vol.-% CO • Equilibration time is max. 0.5 hours. (chamber with O control) •...
Required gas supply for the chamber with O control In the chamber with O control, cell growth can be additionally influenced by a variable oxygen content. Depending on the required range of O concentration, the type of gas which is required may vary. To this end, two equipment variants are available: 6.7.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O...
Activating / deactivating CO and humidity control If CO control and/or O control (chamber with O control) and/or humidity control (chamber with active humidification) shall not be used, it is possible to deactivate them in the controller. Press the Setpoint setting icon to access the “Setpoint” setting menu from Normal display. “Setpoints”...
If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance. CB (E7) 06/2018...
Set-point entry in “Fixed value” operating mode In Fixed value operating mode you can enter set-points for temperature, CO and O (chamber with O control) and humidity (chamber with active humidification), and the switching-state of up to 16 operation lines. All settings made in Fixed value operating mode remain valid until the next manual change.
Set-point entry through the “Setpoints” menu Press the Setpoint setting icon to access the “Setpoint” setting menu from Normal display. “Setpoints” menu. Select “Fixed value operation setpoints” to access the individual parameters. • Select the field “Temperature” and enter the desired temperature setpoint. Setting range: 20 °C up to 60 °C.
Direct setpoint entry via Normal display Alternatively you can also enter the setpoints directly via Normal display. Normal display. Select the setpoint you want Example: “Temperature” entry menu. Enter the to change. desired setpoint and confirm entry with Confirm icon. CB (E7) 06/2018 Page 59/174...
• Operation line “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with O control) • Operation line “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other operation lines are without function.
Safety instructions for setting high gas concentrations Notes on handling carbon dioxide (CO Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system.
Timer program: stopwatch function During an entered duration the controller constantly equilibrates to the setpoints entered in Fixed value operation mode (temperature, CO (chamber with O control), humidity (chamber with active humidification), configuration of the operation lines). This duration can be entered as a “Timer program”. During the program runtime, any setpoint changes do not become effective;...
Stopping a running timer program 8.2.1 Pausing a running timer program Press the Program pause icon to interrupt the program. The program is paused. The program runtime stops running down, the time display flashes. There are the following options: Press the Program start icon to continue the program Press the Cancelling icon to cancel the program 8.2.2 Cancelling a running timer program...
Time programs The MB2 program controller permits programming time programs with real-time reference. It offers 25 program memory positions with up to 100 program sections each. (chamber with O control), For each program section you can enter set-points for temperature, CO and humidity (chamber with active humidification), section duration, type of set-point transition (ramp or step) and the tolerance ranges.
9.1.1 Performance during program delay time During the configured program delay time until program start, the controller equilibrates to the current setpoints of Fixed value operation mode. Modifications of these setpoints are effective. When the configured moment for program start is reached, the program delay time ends and the program starts running.
Creating a new time program Path: Main menu > Programs > Time program “Time program” menu: Enter the program name and, if desired, overview of the existing programs. additional program information in the corresponding fields. Select an empty program place. Press the Confirm icon.
Program editor: “Edit program” menu Select the desired function and press the Confirm icon. The program editor offers following options: • Change the program name • Copy program • Replace program: Replacing an new or an existing program with the copied program. This menu point is visible only after a program has been copied.
Section editor: section management Path: Main menu > Programs > Time program Select the desired program. Program view. Section view (example: section 1). Select the desired program section There are the following options: (example: section 1) Select a parameter to enter or modify the according value (chap.
9.6.1 Add a new program section Section editor: “Edit section” menu. Select “Create new section” and press the Confirm icon. Then select whether to insert the new section before or after the current section. Press the Confirm icon. The new section opens. 9.6.2 Copy and insert or replace a program section Program view.
Press the Edit icon to open the section editor if you want the current section to be replaced or the copied section to be inserted before or after it Program view. Section view (example: section 1). Select the section to be replaced or before or Press the Edit icon to open the section editor after which the copied section shall be inserted (example: section 2) and press the...
Value entry for a program section Path: Main menu > Programs > Time program Select the desired program and section. The section view gives access to all parameters of a program section. You can enter or modify the values. Program name and section number Section duration Type of setpoint transition: ramp or step Operation lines...
9.7.2 Set-point ramp and set-point step You can define the type of transitions for temperature, CO concentration, O concentration (chamber with O control) and humidity (chamber with active humidification) for each individual program section. “Ramp” mode: Gradual set-points changes The set-point of a given program section functions as the section’s start value. During the section’s duration, each set-point gradually passes to the set-point of the subsequent program section.
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Selecting the setting “Ramp” or “Step” Section view (partial view). In the field “Course” select the desired setting “Ramp” or “Step”. “Ramp” and “Step” mode example (representation of a temperature course) ------------1-------------------------2------------------------3------------4----------5------------ W/°C 40.0 39.5 39.0 38.5 38.0 37.5 37.0 t/min.
• Operation line “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with O control) • Operation line “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other operation lines are without function.
9.7.4 Setpoint entry • Select the field “Temperature” and enter the desired temperature setpoint. Setting range: 20 °C up to 60 °C. Confirm entry with Confirm icon. The controller returns to the section view. • Select the field “CO2” and enter the desired CO setpoint.
• Select the field “Tolerance band min” and enter the desired lower tolerance band value. Setting range: -99999 to 99999. Confirm entry with Confirm icon. The controller returns to the section view. • Select the field “Tolerance band max” and enter the desired upper tolerance band value. Setting range: -99999 to 99999.
9.7.7 Saving the time program Section view. Program view. After the all desired values of the program Press the Confirm icon to take over the section have been configured, press the programming. Confirm icon to take over the programming. The controller changes to the Normal display. The controller changes to the program view.
Week programs The MB2 program controller permits programming week programs with real-time reference. It offers 5 week program places in total with up to 100 shift points for each week program. Path: Main menu > Programs> Week program 10.1 Starting an existing week program In Normal display press the Program start icon to access the “Program start”...
10.3 Creating a new week program Path: Main menu > Programs > Week program Enter the program name and, if desired, “Week program” menu: additional program information in the overview of the existing programs. corresponding fields. Select an empty program place. Select the set-point course “Ramp”...
10.4 Program editor: program management Path: Main menu > Programs > Week program “Week program” menu: Program view (example: program 1). overview of the existing programs. If a new program has been created, there is Select an existing program just one program section.
Program view. To add a new section, select “Create new With a new section no weekday is specified. section” and press the Confirm icon. Therefore the section is first marked in red and The program view opens. cannot be saved. A new section is always added at the very bottom (example: section 3).
10.5 Section editor: section management Path: Main menu > Programs > Week program Select the desired program. Program view. Section view (example: section 1). Select the desired program section There are the following options: (example: section 1) ...
10.5.1 Add a new program section Section editor: “Edit section” menu. Program view. Select “Create new section” and press the With a new section no weekday is specified. Confirm icon. Therefore the section is first marked in red and cannot be saved. A new section is always added at the very bottom (example: section 3).
Select “Replace section” to replace the selected section with the copied section Select “Insert section” to additionally add the copied section. Press the Confirm icon. If you selected “Insert section” the sections are automatically arranged in the correct chronological order. Section editor: “Edit section”...
“Change program name” menu. In the field “Course” select the desired setting “Ramp” or “Step” and press the Confirm icon. 10.6.2 Weekday In the field “Weekday” select the desired weekday. With “Daily” selected, this section will run every day at the same time. Section view.
• Operation line “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with O control) • Operation line “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other operation lines are without function.
Notification and alarm functions 11.1 Notification and alarm messages overview 11.1.1 Notifications Notifications are indicated by information icons displayed in the screen header in Normal display An information icon serves as an indication of a certain condition. If this condition persists, in some cases an alarm will be triggered after a fix or configurable interval. As long as the condition persists, the information icon therefore continues to be displayed also in state of alarm.
11.1.2 Alarm messages Start after Zero-voltage relay Condition Alarm message condition occurred alarm output Having reached the setpoint, the actual temperature value deviates by more than the set tolerance range value and longer than the set delay After configurable time from the setpoint “Temperature delay time (chap.
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“CO2 sensor fault” immediately immediately BINDER Service. Chamber with O control: “O2 sensor fault” immediately immediately sensor defective. Contact BINDER Service Chamber with active humidification: “Humid sensor fault” immediately immediately Humidity sensor defective. Contact BINDER Service Hot air sterilization running “Sterilization”...
11.2 State of alarm 1. Visual indications in Normal display: alarm message, screen header flashing in red color 2. Audible alert, if the buzzer is enabled (chap. 11.4). 3. In some cases: Switching the zero-voltage relay alarm output (chap. 11.6) to transmit the alarm e.g., to a central monitoring system.
11.4 Activating / deactivating the audible alarm (alarm buzzer) Path: Main menu > Settings > Chamber “Chamber” submenu (example). In the field “Audible alarm” select the desired setting “off“ or “on” and press the Confirm icon. 11.5 Tolerance range settings and alarm delay times Path: Main menu >...
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Alarm message Start after condition occurred “Temperature range” after configurable time “CO2 range after configurable time “O2 range after configurable time “Humidity range after configurable time “Door open” after configurable time If the condition (deviation from the tolerance range or door opening) ends within the configured alarm delay time, no alarm will be triggered after the delay time.
11.6 Zero-voltage relay alarm output The chamber is equipped with a zero-voltage relay output which permits the transmission of some alarm messages to a central monitoring system. The connection is realized as a DIN socket (3) on the chamber rear. A suitable DIN plug is enclosed.
The user cannot restart the device again. The protective cut-off device is located internally. Only a service specialist can replace it. Therefore, please contact an authorized service provider or BINDER Service. 12.2 Overtemperature safety controller class 3.1 The chambers are regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880:2007).
12.2.1 Safety controller modes You can select between “Limit (absolute)” and “Offset (relative)” safety controller mode • Limit: Absolute maximum permitted temperature value This setting offers high safety as a defined temperature limit will not be exceeded. It is important to adapt the safety controller set-point after each modification of the temperature set-point.
Regularly check the safety controller setting for set-point type “Limit” or “Offset” • in Fixed value operating mode according to the entered set-point temperature value • in program mode according to the highest temperature value of the selected temperature program Set the safety controller set-point by approx.
• All passwords can be changed in the “log out” submenu (chap. 13.3). “Service” authorization level • Authorization level only for BINDER service • Extensive authorization for controller operation and configuration, access to service data • The passwords for “Service”, “Admin” and “User” authorization levels can be changed in the “log out”...
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Operation after user login At user login, the authorization level is selected and confirmed by entering the respective password. Following user login, controller operation is available, recognizable by the open-lock icon in the header. The available controller functions correspond to the user’s authorization level. Password protection activated for all levels: operation without user login is locked If passwords have been assigned for all authorization levels, the controller is locked without...
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Information window To check the authorization level of the user currently logged-in, select in Normal display the arrow far right in the display header. The information window shows date and time, the controller’s free memory space and under “Authorization” the authorization level of the current user. If passwords have been assigned for all authorization levels, a user without login (password entry) has no authorization.
13.2 Log in Path: Main menu > User > Log in Controller without a user logged-in Selection of user type (example) All selection possibilities are password protected Controller with logged-in user After completing the settings, press the Confirm icon to take over the entries and exit the menu, or press the Close icon to exit the menu without taking over the entries.
13.3 Log out Path: Main menu > User > Log out User logoff with “Admin” authorization Controller Controller with without a user logged-in user logged-in User logoff with “Admin” authorization Controller Controller with without a user logged-in user logged-in 13.4 User change If the password function has been deactivated (chap.13.5.2) this function is not available.
User selection (example) All selection possibilities are password protected Controller with logged- in user 13.5 Password assignment and password change This function is not available for a user logged-in with “User” authorization. 13.5.1 Password change A logged-in user can change the passwords of his current level and of the next lower level(s). Example: A user with “Admin”...
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Selection of the authorization level Enter desired password. If desired, press the Change (example: view with “Admin” authorization) keyboard icon to access other entry windows. In the “Keyboard switch” window you can select different keyboards to enter uppercase and lowercase letters, digits, and special characters.
13.5.2 Deleting the password for an individual authorization level A user logged-in with “Service” or “Admin” authorization can delete the passwords of his current level and of the next lower level(s). To do this no password is entered during a password change. Path: Main menu >...
13.5.3 New password assignment for “service” or “admin” authorization level when the password function was deactivated If the password protection for an authorization level has been deactivated, i.e., no password is assigned, no login for this level is possible. Therefore access to this authorization level is available without login. If the password for the “Service”...
13.6 Activation code Certain functions of the controller can be unlocked with a previously generated activation code. The activation code enables access to functions available only in the “Service” authorization level by users without a “Service” authorization. Such functions include e.g., adjustment or extended configurations.
General controller settings Most of the general settings can be accessed in the “Settings” submenu, which is available for users with “Service” or “Admin” authorization level. It serves to enter date and time, select the language for the controller menus and the desired temperature unit and to configure the controller’s communication functions.
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Or later: Path: Main menu > Settings > Date and time “Date and time” submenu. “Date / time” entry menu. Select the field “Date / time”. Enter date and time and press the Confirm icon. “Date and time” submenu. “Date and time” submenu. In the field “Daylight saving time switch”...
14.3 Selecting the temperature unit Following start-up of the chamber: Or later: Path: Main menu > Settings > Chamber Select the desired temperature unit and press the Confirm icon. Change of the temperature unit between °C and °F. If the unit is changed, all values are converted accordingly C = degree Celsius 0 °C = 31°F...
• Select the field “Brightness”. Move the grey slide to the left or right to define the brightness of the display • left = darker (minimum value: 0) • right = brighter (maximum value: 100) Press the Confirm icon. • Select the field “Wait time for screen saver” and enter the desired waiting time for the screen saver in seconds.
This menu allows to configure the communication parameters of the RS485 interface. The device address is required to recognize chambers with this interface type in a network, e.g. when connecting it to the optional communication software BINDER APT-COM™ 3 DataControlSystem (chap. 18.1). In this case do not change the other parameters.
14.5.2 Ethernet 14.5.2.1 Configuration Path: Main menu > Settings > Ethernet “Ethernet” submenu. • In the field “IP address assignment” select the desired setting “Automatic (DHCP)“ or “Manual“. With selection “Manual” you can enter the IP- address, the subnet mask and the standard gateway manually.
Internet. The IP address is available via Chamber information > Ethernet. The BINDER web server opens. Enter the user name and password which have been assigned for the web server in the controller menu. This enables online access to the controller display, to see e.g., the event list or error messages.
14.5.4 E-Mail As soon as an alarm was triggered, an e-mail is sent to the configured e-mail address. Path: Main menu > Settings > Email E-mail address entry: “Email” submenu. Select the desired e-mail address field and enter the e-mail address. You can use the Keybord change icon for entry.
14.6 USB menu: Data transfer via USB interface The USB port is located in the instrument box. When you insert a USB-stick, the “USB” menu opens. Depending on the user’s authorization level, different functions (highlighted in black) are available for the logged-in user.
General information 15.1 Service contact page Path: Main menu > Contact 15.2 Current operating parameters Press the Information icon to access the “Info” menu from Normal display. “Info” menu. Select the desired information. • Select “Program operation” to see information on a currently running program. •...
(chap. 16.2) the Event list is cleared. 15.4 Technical chamber information Path: Main menu > Device info Chamber name and setup Versions of CPU, I/O module and safety for BINDER controller Service Information on digital and analog inputs and for BINDER outputs and phase angle outputs...
Chart recorder display This view offers graphic representation of the measurement course. Data representation imitates a chart recorder and allows recalling any set of measured data at any point of time taken from the recorded period. 16.1 Views Press the Change view icon to access the pen recorder display. 16.1.1 Show and hide legend Show legend...
16.1.3 Show and hide specific indications Show indications Hide indications Press the Show indications icon to display the indication “Door open” (B2). Indication “Door open” displayed. 16.1.4 History display History display Press the History display icon to change to the history display. History display.
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History display: Curve selection Curve selection Press the Curve selection icon to access the “Curve selection” submenu. “Curve selection” submenu. Select the curves to be displayed by checking the checkbox of the corresponding parameter. Press the Confirm icon History display: Search the required instant Search Press the Search icon to access the “Search”...
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History display: Zoom function Zoom Press the Zoom icon to access the “Zoom” submenu. “Zoom” submenu. Select the zoom factor and press the Confirm icon History display: Show and hide scroll buttons to scroll to an instant Show scroll buttons Hide scroll buttons Press the Show scroll buttons icon to access the “Page selection”...
16.2 Setting the parameters This menu allows setting the storage interval, the type of values to be shown and the scaling of the temperature and humidity charts. Path: Main menu > Settings > Measurement chart “Measurement chart” submenu. • Select the field “Storage interval” and enter the desired storage interval. Confirm entry with Confirm icon.
• Water intake temperature NOT below +5 °C / 41 °F and not exceeding 40 °C / 104 °F. BINDER GmbH is NOT responsible for the water quality provided by the customer. Any problems and malfunctions that might arise following use of water of deviating quality is excluded from liability by BINDER GmbH.
Options 18.1 Communication software APT-COM™ 3 DataControlSystem (option) The chamber is regularly equipped with an Ethernet interface (5) that can connect the BINDER communication software APT-COM™ 3 DataControlSystem. The actual values of temperature, CO (chamber with O control) and humidity (chamber with active humidification) are given at adjustable intervals.
Risk of burns and other injuries. Tightly close each access port with two plugs during operation. 18.3 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) The interior socket is activated and deactivated in the controller via the switching state of an operation line (chap.
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The maximum head load must not exceed a capacity of 20 W. CAUTION Exceeding the setpoint temperature. Damage to the cultures. ∅ Do NOT exceed the maximum head load of 20 W. ∅ Do NOT connect equipment with a nominal capacity > 20 W. Heat emission of electrical devices connected inside the CO incubator may modify the temperature range.
BINDER Data Loggers are equipped with a keyboard and a large LCD display, alarm functions and a real-time function. Measurement data are recorded in the Data Logger and can be read out after the measurement via the RS232 interface of the Data Logger.
18.6 Access port for extra-low voltage (option) The access port (B) (8-pin) for extra-low voltage (ELV) consists of a LEMO socket (which can be covered) and a LEMO connector. It is suitable to connect two electrical devices, one inside and one outside the incubator.
(chap. 21.3). The mounting instructions 7001-0145 delivered with the stacking adapter describe its installation (Art. No. 9051-0032 for CB 56, Art. No. 9051-0026 for CB 170, Art. No. 9051- 0030 for CB 260).
BINDER recommends using the base on castors. The mounting instructions 7001-0147 delivered with the base on castors describe its installation (Art. No. 9051-0031 for CB 56, Art. No. 9051-0028 for CB 170, Art. No. 9051- 0029 for CB 260).
All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10 % of the full-scale value. These test systems are not suitable for calibrating the BINDER sensor system. 19.1.3...
Avoiding microbial contamination The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, mycoplasma, and viruses. This chapter gives an overview of potential sources of contamination and precautions and measures to eliminate them. 20.1 Cells and media •...
Condensation in the inner chamber represents a particular risk of contamination. The Permadry™ system developed by BINDER is an effective and easy way to ensure high humidity (≥95 % r.h.) inside the chamber without any condensation forming on the inner surfaces.
• The chamber should be calibrated / precisely adjusted. • Let BINDER Service adjust the setting of the door heating in reaction to critical ambient conditions. Regular cleaning, decontamination and sterilization • Clean the shelves, glass door, gaskets, and inner chamber weekly (for cleaning see chap. 21.1, for decontamination see chap.
We recommend using the neutral cleaning agent Art. No. 1002-0016 for thorough cleaning. Any corrosive damage that may arise following use of other cleaning agents is excluded from liability by BINDER GmbH. Any corrosive damage caused by a lack of cleaning, is excluded from liability by BINDER GmbH. CAUTION Danger of corrosion.
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∅ Do NOT spray cleaning detergents directly on the CO sensor. Wipe the sensor with a lint-free cloth soaked with cleaning agent. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the sensor.
∅ Do NOT spray the disinfectant directly on the CO sensor. Wipe the sensor with a lint-free cloth soaked with the disinfectant. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the sensor.
Recommended precautions: To protect the eyes use sealed protective goggles. CAUTION Eye contact. Eye damage due to chemical burns. ∅ Do NOT empty into drains. Wear protective goggles. Following frequent use of the disinfectant spray and prior to hot-air sterilization, remove any agent remainder by using the neutral cleaning agent and then a moistened towel to avoid formation of permanent residues.
Note: in the case when two chambers are directly stacked on top of each other without using the original BINDER stacking stand or the BINDER stacking adapter, the exact maintenance of the incubation set parameters in one of the chambers while carrying out sterilization in the other one cannot be assured.
21.3.3 Starting the sterilization cycle and running the hot-air sterilization In Normal display press the Program start icon to access the “Program start” menu. “Program start” menu • In the field “Program type” select the setting “Time program”. • In the field “Program” select the program “Sterilization”. •...
Opening the outer door leads to prematurely terminating the running sterilization cycle. If this happens during the heating up or holding phase (during 4 hours after starting the sterilization cycle), sterilization will be ineffective (chap. 21.3.4.1). CAUTION Opening the outer door during the heating up or holding phase. Interruption of the temperature reaction time.
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective sterilization After prematurely terminating the sterilization cycle after less than 4 hours, the information “Sterilization failed” is shown on the controller display. The interior may still be hot. Do not touch the glass door and inner surfaces! Confirm the message with the Confirm icon.
The information “Successful sterilization” on the controller display indicates a successful hot-air sterilization. The interior may still be hot. Do not touch the glass door and inner surfaces! Confirm the message with the Confirm icon. After confirming the message the controller changes to Fixed value operation mode. The setpoints of Fixed value operation mode are then equilibrated.
Replace the door gasket only when cold. Otherwise, the door gasket may become damaged. We recommend taking out a maintenance agreement. Please consult BINDER Service. BINDER telephone hotline:...
When using gas with a technical grade of 99.5 %, we recommend changing the fine gas filter once a year. Please consult BINDER Service. When using gases with less pureness, the changing intervals should be shorter. 22.4 Replacing the CO sensor Pull the power plug.
22.5 Sending the chamber back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us.
Disposal 23.1 Disposal of the transport packing 23.1.1 Outer chamber packing Packing element Material Disposal Straps to fix packing Plastic Plastic recycling on pallet (no image) Shipping box Cardboard Paper recycling Edge stuffing, top PE foam Plastic recycling PE foam Plastic recycling Pallet with foamed plastic stuffing...
(Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl. I p. 1739) or contact BINDER service who will organize taking back and disposal of the chamber according to the German national law for electrical and electronic equipment (Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl.
According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control instruments” (category 9) only intended for professional use“. They must not be disposed of at public collecting points.
Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to Directive 2012/19/EU. The devices must be free from toxic, infectious or radioactive substances in order to eliminate any health hazards to the employees of the recycling companies.
Troubleshooting Only qualified service personnel authorized by BINDER must perform repair. Repaired chambers must comply with the BINDER quality standards. 24.1 General Fault description Possible cause Required measures No power supply. Check connection to power supply. Check power supply for voltage of 100- Wrong voltage.
Temperature sensor Transfer the cultures to another reference method.. defective. incubator and contact BINDER Service. 24.3 Gas cylinder pressure too low The alarm messages indicate that the pressure in the supply lines of CO (chamber with O control)
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Controller malfunction. controller. If necessary, contact BINDER Service. Pressure sensor system Acknowledge the alarm on the defective. controller. Contact BINDER Service. Acknowledge the alarm on the cylinder is not connected controller. Correctly connect the gas correctly. cylinder. Connected gas cylinder is Acknowledge the alarm on the closed.
Acknowledge the alarm on the sensor defective. controller. Contact BINDER Service. Recovery time (up to 5 vol.-% ) after doors were open for sensor system defective. Contact BINDER Service. 2 minutes is < 2 minutes CB (E7) 06/2018 Page 154/174...
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The Transfer the cultures to another pH indicator of the cell culture sensor defective. incubator and contact BINDER Service. medium changes its normal color. Chamber with O control: control not adjusted. Calibrate and adjust O control.
Doors not closed properly. Close door properly. Humidity set too high. Reduce humidity (chap. 6.9) Place the chamber on a BINDER Chamber placed on very cold stand to increase the distance to the floor. floor. Condensation on the doors or Door gaskets defective.
Main power switch turned off. Turn on the main power switch. Log in with the required higher Menu functions not available with authorization. or contact BINDER Menu functions not available. current authorization level. service to obtain an activation code (chap. 13.6).
(certified since December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals.
25.3 Definition of usable volume The usable volume illustrated below is calculated as follows: A, B, C = internal dimensions (W, H, D) a, b, c = wall separation a = 0.1*A b = 0.1*B c = 0.1*C = (A - 2 * a) * (B - 2 * b) * (C - 2 * c) Figure 30: Determination of the usable volume The technical data refers to the defined usable volume.
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Chamber size Shelves Number of shelves, regular Number of shelves, max. mm x mm 396 x 289 551 x 442 614 x 502 Size of shelf (external) Width x Depth inch x inch 15.59 x 11.38 21.69 x 17.40 24.17 x 19.76 Maximum load per shelf Kg / lbs.
25.5 Equipment and Options (extract) To operate the chamber, use only original BINDER accessories or accessories / components from third-party suppliers authorized by BINDER. The user is responsible for any risk arising from using unauthorized accessories. Regular equipment...
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INDIVIDUAL Customized Solutions) Freshwater bag (0.9 liters / 0.32 cu.ft.) Divided inner door, gas-proof, stainless steel, 4 times (CB 56), 6 times (CB 170), 8 times (CB 260) Shelves for divided inner door, gas-proof, stainless steel Stacking stand with castors lockable by breaks (CB 170 / CB 210)
25.6 Accessories and spare parts (extract) BINDER GmbH is responsible for the safety features of the chamber only, provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
8012-1550 Spatial temperature measurement including certificate (27 measuring points) 8012-1592 For information on components not listed here, please contact BINDER Service. 25.7 Important conversion data for non-SI units 1 ft = 0.305 m 1 m = 100 cm = 3.28 ft = 39.37 inch 1 mbar = 0.0145 psi...
Contamination clearance certificate 28.1 For chambers located outside the USA and Canada Declaration regarding safety and health Erklärung zur Sicherheit and gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace, require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed.
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Kind of transport / transporter / Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) ___________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ CB (E7) 06/2018 Page 170/174...
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We are aware that, in accordance with Article 823 of the German Civil Code (BGB), we are directly liable with regard to third parties, in this instance especially the employees of BINDER GmbH, who have been entrusted with the handling / repair of the chamber / component. / Es ist uns bekannt, dass wir gegenüber...
Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA number. Please be aware that size specifications, voltage specifications as well as performance www.binder-world.us...
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Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is completed by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
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4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc. for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.
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