Appendix A - Reagents; Best Practices For Solution Preparation: Vitamin & Cholesterol Analysis; Best Practices For Solution Preparation: Fat Analysis - Ankom Flex Analyte Extractor Operator's Manual

Operator's Manual
16. Appendix A – Reagents
Best practices for solution preparation: Vitamin & Cholesterol Analysis
Where high volume usage makes daily solution preparation less practical, the following guidelines can be used.
Solution Reservoir
Ethanol 1. Green
12.7N KOH 2. Yellow
Water 3. Blue
Hexane 4. Red

Best practices for solution preparation: Fat Analysis

Where high volume usage makes daily solution preparation less practical, the following guidelines can be used.
Solution Reservoir
Ethanol 1. Green
3N HCl 2. Yellow
Water 3. Blue
Hexane 4. Red
pg. 48
Solution
Make up
Content
2% (w/v) Weigh 8g ± 0.1g
Pyrogallol Pyrogallol
(or equivalent) (or equivalent) into
in Ethanol 500ml volumetric flask.
Make up to mark with
95% Ethanol
12.7N KOH 500g KOH + 500ml water
Water Deionized water
0.05g/L BHT in 0.05g Butylated
Hexane hydroxytoluene (BHT) +
1L Hexane
Solution
Make up
Content
2% (w/v) Weigh 8g ± 0.1g
Pyrogallol Pyrogallol
(or equivalent) (or equivalent) into
in Ethanol 500ml volumetric flask.
Make up to mark with
95% Ethanol
3N HCl
Water Deionized water
0.05g/L BHT in 0.05g Butylated
Hexane hydroxytoluene (BHT) +
1L Hexane
Storage
Shelf Life
Requirements
Make up fresh Actinic glass, Vented
daily. Reservoir, Room
Temperature (15-
o
25 C)
Replenish as Unvented Reservoir,
needed. Clean Room Temperature
bottle every two (15-25
weeks.
Replenish as Vented Reservoir,
needed. Clean Room Temperature
bottle every two (15-25
weeks.
Replenish as Unvented Reservoir,
needed. Clean Room Temperature
bottle every two (15-25
weeks.
Storage
Shelf Life
Requirements
Make up fresh Actinic glass, Vented
daily. Reservoir, Room
Temperature (15-
o
25 C)
Unvented Reservoir,
Room Temperature
(15-25
Replenish as Vented Reservoir,
needed. Clean Room Temperature
bottle every two (15-25
weeks.
Replenish as Unvented Reservoir,
needed. Clean Room Temperature
bottle every two (15-25
weeks.
o
C)
o
C)
o
C)
o
C)
o
C)
o
C)
Rev 1/22/2021