Ankom TDF Operator's Manual
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Ankom TDF Operator's Manual

Dietary fiber analyzer
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Operator's Manual
Rev 9/20/18
2052 O'Neil Rd, Macedon NY 14502
Telephone: (315) 986-8090
Fax: (315) 986-8091
www.ankom.com

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  • Page 1 Operator’s Manual Rev 9/20/18 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.com...
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  • Page 3: Table Of Contents

    Dietary Fiber Analyzer ................... 16 Blanks ..................................16 IDF/SDF Analysis ................................. 17 TDF Analysis (AOAC 991.43) ............................31 TDF Analysis (AOAC 985.29 / 2001.03) ........................44 Protein Determination ..............................59 Ash Determination ..............................61 Productivity Enhancement ............................63 Status Screen ................................67 Fault Handling ................................
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  • Page 5: Introduction

    ANKOM Technology or from your local authorized ANKOM distributor. Contact Information Committed to your total satisfaction, we are always available to help you get the most from your ANKOM products and to receive any comments or suggestions you might have to help us improve.

  • Page 6: Method Comparison (Manual Vs. Automated)

    Dietary Fiber Analyzer. Steps highlighted in YELLOW represent tasks done by a technician. Steps highlighted in GREEN (1a-1g, 9, 19, and 30) are no longer needed because of Filter Bag Technology. All other steps (including mixing) are done automatically by the ANKOM Dietary Fiber Analyzer under computer control.

  • Page 7: Instrument Description

    The automated fiber recovery is achieved by first digesting samples within bags using enzyme treatments. Depending on the filter bag configuration, the instrument then collects the IDF and SDF residue using two separate filters, or collects the TDF residue using one filter. Once captured in the filters the residue is dried, weighed, and corrected for protein and ash content to determine the IDF, SDF, and/or TDF values.
  • Page 8 Operator’s Manual External Components – Left Side View Power Switch Front Cover Arm Front Cover (TDF56) Enzyme Containers (TDF35, TDF36, TDF37) Pressure Gauge Nitrogen Supply Port 80-100 psi HCl Container (TDF38) (5.5-6.9 bar) Single Container Holder External Components – Right Side View Front Cover Arm 78% EtOH Container (TDF41) Control Panel (TDF17)
  • Page 9 Operator’s Manual Internal Components – Rear View In-Line Heater Main Electrical Cabinet IDF Inlet Valve Stations 4, 5 & 6 IDF Inlet Valve SDF Inlet Valve Tubing Support Panel Stations 1, 2 & 3 Stations 1, 2 & 3 Torsion Bar (for SDF Inlet Valve Front Cover) Stations 4, 5 &...

  • Page 10: Service Access Position

    WARNING: Attempts to override safety features or to use this instrument in a manner not specified by ANKOM Technology voids the warranty and may result in serious injury or even death. This system is designed to meet and/or exceed the applicable standards of CE and CSA.

  • Page 11: Instrument Installation

    Because the instrument is powered by pneumatics, do NOT run it without an adequate Nitrogen Supply. You can run approximately 5 to 10 full assays (IDF/SDF or TDF) with a 304 cu ft tank of nitrogen. Instrument Installation Procedure To install the ANKOM Dietary Fiber Analyzer, follow the steps below.
  • Page 12 Operator’s Manual 1 – Pinch Valve Tubing Set (TDF71) 1 – Long Life Pump Tube Set (TDF99) 1 - Line Flush Tubing assy (TDF70) 1 – Amylase Container assy (TDF35) 1 – Protease Container assy (TDF36) 1 – AMG Container assy (TDF37) 1 –...
  • Page 13 Operator’s Manual 2 – Container Filters (8202) 1 – Buffer Filter (8203) 4 – Bolts (5626) 4 – Nuts with star washers (167) 4 – Acorn Nuts (8409) 1 – Lighted Magnifier (TDF45) 2 – Fuses (8305-15A or 8306-8A) Accessories specific to the 2009.11 & 2011.25 method upgrades can be found in the "Automated AOAC 2009.01/2011.25 and AACC 32-45.01 method"...
  • Page 14 25 pin connector Place the Chemical Containers in their holders. If you are going to run an IDF/SDF or TDF analysis immediately after installing the instrument, fill the Chemical Containers above the Minimum Level lines and fill the Enzyme Containers with at least 15 ml of solution before connecting the containers to the instrument.
  • Page 15 Operator’s Manual Connect the Enzyme Containers to the corresponding ports. The Enzyme Containers connect into labeled, color-coded ports on the left side of the instrument using push-on connectors. Make sure that you hear a click sound when you connect the enzyme containers to the ports. Push-on Connector Connect the Nitrogen Supply to the Nitrogen Supply Port.

  • Page 16: Dietary Fiber Analysis Support Items

    BLANK values are used in the IDF, SDF, and TDF calculations. The following BLANK values (based on extensive research done in the ANKOM lab) are the default values loaded in the IDF_SDF and TDF spreadsheets in the MS-Excel workbook provided with the instrument.

  • Page 17: Idf/Sdf Analysis

    Operator’s Manual IDF/SDF Analysis An IDF/SDF analysis measures the amount of IDF and SDF within a given sample. This requires the use of ANKOM DF-I and DF-S filter bags. When starting a new run, the ANKOM Dietary Fiber Analyzer must have bags installed at all stations (a total of twelve bags).
  • Page 18 When you turn the power on, the instrument will run through an initialization process and the Control Panel will turn on. On the Touch Screen Display, select the instrument function you would like to perform. The Control Panel on the ANKOM Dietary Fiber Analyzer uses Touch Screen technology. To operate the instrument you will press identified buttons on the Touch Screen Display and buttons below the screen.
  • Page 19 Operator’s Manual Press the Line Charge button. The following screen will be displayed. 9.1.2 Vacuum xx.x Line Charge ← cur: - x.x max: - x.x Amylase Protease EtOH78 EtOH95 Water Buffer Press the ALL button to charge all of the lines. Each button will change color as the associated line is being 9.1.3 charged.
  • Page 20 Operator’s Manual 10. Install SDF filter bags on the ANKOM Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C, and D by lifting them off of the locator rods. Clamp Bar A Clamp Bar B Clamp Bar C Clamp Bar D 10.2...
  • Page 21 Diatomaceous Earth added in SDF Bags?  11. Add DE to the SDF filter bags on the ANKOM Dietary Fiber Analyzer. 11.1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle.
  • Page 22  After you confirm that the DE is added, the Clamp Bar D button on the Control Panel is disabled until the SDF process completes. 12. Install IDF filter bags on the ANKOM Dietary Fiber Analyzer. 12.1 Gently pull the black IDF Delivery Nozzle out toward you.
  • Page 23 Operator’s Manual 12.4 Place at least 20 mm (0.75 inches) of the filter section of the IDF bag inside the top of the SDF bag to allow for the flow of liquid into the SDF bag after the IDF process is complete. Heating Paddle Top part of the filter is Clamp Bar B...
  • Page 24 Operator’s Manual 13.2 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer. Repeat steps 13.1 – 13.2 for all IDF stations. 13.3 13.4 Re-install Clamp Bar A by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument.
  • Page 25 Operator’s Manual AOAC ← 991.43 Check supply containers IDF/SDF Amylase -> Amylase -> AMG -> HCL Buffer -> Mes Tris Buffer  15. Verify that the fluid containers have the correct fluids for this procedure. Verify that the fluid supply containers are configured according to the screen above and press the check mark () button. The following screen will be displayed.
  • Page 26 Operator’s Manual 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in your lab is connected to the instrument and turned on. The pressure gauges on the instrument should show 50-55 psi on the left and about 4 psi on the right. Press the check mark () button on the Touch Screen Display.
  • Page 27 Operator’s Manual AOAC ← 991.43 IDF/SDF Press START to begin START 20. Start the instrument. The instrument is now completely set up and ready to automatically run the IDF/SDF procedure. Press the START button to begin. At the beginning of each new run, the instrument automatically runs a tube integrity test. For the first run after a power-up cycle, or for the first run after the instrument has been idle for twelve hours, the following screen will be displayed.
  • Page 28 After the instrument has completed an IDF process the IDF residue that has been collected in the IDF bag must be manually rinsed twice with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand (sold separately) for rinsing filter bags with acetone.
  • Page 29 After the instrument has completed an SDF process the SDF residue that has been collected in the SDF bag must be manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand for rinsing filter bags with acetone.
  • Page 30 Protein of Blank Filter Bag (g) Ash of Blank Filter Bag (g) Original wt of Diatomaceous Earth in Blank Filter Bag (g) 31. Calculate the % TDF value by adding the % IDF and % SDF values. pg. 30 Rev 9/20/18...

  • Page 31: Tdf Analysis (Aoac 991.43)

    TDF Analysis (AOAC 991.43) A TDF analysis directly measures the amount of TDF within a given sample without separately measuring the IDF and SDF fractions. TDF can be determined using multiple methods. This section describes the procedure for using the ANKOM Dietary Fiber Analyzer to determine TDF based on the AOAC 991.43 method.
  • Page 32 When you turn the power on, the instrument will run through an initialization process and the Control Panel will turn on. On the Touch Screen Display, select the instrument function you would like to perform. The Control Panel on the ANKOM Dietary Fiber Analyzer uses Touch Screen technology. To operate the instrument you will press identified buttons on the Touch Screen Display and buttons below the screen.
  • Page 33 AOAC 991.43 AOAC 985.29 IDF/SDF AOAC 991.43 AOAC 2001.03 Diagnostics Press the AOAC 991.43 TDF button. The following screen will be displayed. AOAC ← 991.43 SDF Bags (and clamp bar D) installed?  Notice that the function you selected is now displayed in the top right corner of the screen and a back button is displayed in the top left corner of the screen.
  • Page 34 Operator’s Manual 10. Install SDF filter bags on the ANKOM Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C, and D by lifting them off of the locator rods. Clamp Bar A Clamp Bar B Clamp Bar C Clamp Bar D 10.2...
  • Page 35 Diatomaceous Earth added in SDF Bags?  11. Add DE to the SDF filter bags on the ANKOM Dietary Fiber Analyzer. 11.1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle.
  • Page 36  After you confirm that the DE is added, the Clamp Bar D button on the Control Panel is disabled until the SDF process completes. 12. Install IDF Flow-thru bags on the ANKOM Dietary Fiber Analyzer. 12.1 Gently pull the black IDF Delivery Nozzle out toward you.
  • Page 37 Operator’s Manual 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the top of the SDF bag to allow for the flow of solution into the SDF bag after the digestion process is complete. Heating Paddle 20 mm (0.75 inches) of the IDF Flow-Thru bag inside the...
  • Page 38 Operator’s Manual Repeat steps 13.1 – 13.2 for all IDF stations. 13.3 13.4 Re-install Clamp Bar A by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. 13.5 Press the check mark () button on the Touch Screen Display.
  • Page 39 16. Fill fluid containers. To ensure that you have enough fluids to run a complete TDF procedure, you must begin with fluid levels above the Minimum Level lines on the chemical containers and at least 15 ml of each enzyme. Add fluids and enzymes as necessary.
  • Page 40 Operator’s Manual 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in your lab is connected to the instrument and turned on. The pressure gauges on the instrument should show 50-55 psi on the left and about 4 psi on the right. Press the check mark () button on the Touch Screen Display.
  • Page 41 20. Start the instrument. The instrument is now completely set up and ready to automatically run the TDF procedure. Press the START button to begin. At the beginning of each new run, the instrument automatically runs a tube integrity test.
  • Page 42 23. Rinse the SDF filter bags with acetone. After the instrument has completed a TDF process the TDF residue that has been collected in the SDF bag must be manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand (sold separately) for rinsing filter bags with acetone.
  • Page 43 Repeat steps 25.1 – 25.3 for each SDF filter bag used in the fiber analysis. 25.4 26. Determine the Protein content within the TDF residue. See the “Protein Determination” section of this manual for recommended procedures. 27. Determine the Ash content within the TDF residue.
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  • Page 45: Tdf Analysis (Aoac 985.29 / 2001.03)

    TDF Analysis (AOAC 985.29 / 2001.03) A TDF analysis directly measures the amount of TDF within a given sample without separately measuring the IDF and SDF fractions. TDF can be determined using multiple methods. This section describes the procedure for using the ANKOM Dietary Fiber Analyzer to determine TDF based on the AOAC 985.29 and 2001.03 methods.
  • Page 46 When you turn the power on, the instrument will run through an initialization process and the Control Panel will turn on. On the Touch Screen Display, select the instrument function you would like to perform. The Control Panel on the ANKOM Dietary Fiber Analyzer uses Touch Screen technology. To operate the instrument you will press identified buttons on the Touch Screen Display and buttons below the screen.
  • Page 47 AOAC 991.43 AOAC 985.29 IDF/SDF AOAC 991.43 AOAC 2001.03 Diagnostics Press either the AOAC 985.29 TDF button or the AOAC 2001.03 TDF button depending on the method you're doing. One of the following screens will be displayed. AOAC AOAC ← 985.29 ←...
  • Page 48 Operator’s Manual 10. Install SDF filter bags on the ANKOM Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C, and D by lifting them off of the locator rods. Clamp Bar A Clamp Bar B Clamp Bar C Clamp Bar D 10.2...
  • Page 49 SDF Bags?   11. Add DE to the SDF filter bags on the ANKOM Dietary Fiber Analyzer. 11.1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle.
  • Page 50  After you confirm that the DE is added, the Clamp Bar D button on the Control Panel is disabled until the SDF process completes. 12. Install IDF Flow-thru bags on the ANKOM Dietary Fiber Analyzer. 12.1 Gently pull the black IDF Delivery Nozzle out toward you.
  • Page 51 Operator’s Manual 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the top of the SDF bag to allow for the flow of solution into the SDF bag after the digestion process is complete. Heating Paddle 20 mm (0.75 inches) of the IDF Flow-Thru bag inside the...
  • Page 52 Operator’s Manual Repeat steps 13.1 – 13.2 for all IDF stations. 13.3 13.4 Re-install Clamp Bar A by setting it on the locator rods. Make sure the letter is on the top of the bar and the rubber material is facing in toward the instrument. 13.5 Press the check mark () button on the Touch Screen Display.
  • Page 53 16. Fill fluid containers. To ensure that you have enough fluids to run a complete TDF procedure, you must begin with fluid levels above the Minimum Level lines on the chemical containers and at least 15 ml of each enzyme. Add fluids and enzymes as necessary.
  • Page 54 Operator’s Manual 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in your lab is connected to the instrument and turned on. The pressure gauges on the instrument should show 50-55 psi on the left and about 4 psi on the right. Press the check mark () button on the Touch Screen Display.
  • Page 55 20. Start the instrument. The instrument is now completely set up and ready to automatically run the TDF procedure. Press the START button to begin. At the beginning of each new run, the instrument automatically runs a tube integrity test.
  • Page 56 Operator’s Manual 21. Adjust pH before the Protease digestion. Just before the Protease digestion (after the bags have cooled) the instrument will stop, open Clamp Bar A, display the screen below, and make a sound to remind you to manually adjust pH. pH adjustment Adjust pH to 7.5 +/- 0.2 by adding NaOH solution.
  • Page 57 24. Rinse the SDF filter bags with acetone. After the instrument has completed a TDF process the TDF residue that has been collected in the SDF bag must be manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand (sold separately) for rinsing filter bags with acetone.
  • Page 58 Operator’s Manual 28. Determine the Ash content within the TDF residue. See the “Ash Determination” section of this manual for recommended procedures. 29. Calculate the % TDF value. % TDF )/2] - P - A - B X 100 [(( f...

  • Page 59: Protein Determination

    Operator’s Manual Protein Determination AOAC methods 991.43, 985.29, and 2001.03 suggest that the Kjeldahl method be used for determining protein content. Since a large amount of acid is used in the Kjeldahl digestion process, make sure you use enough base in the Kjeldahl distillation process.
  • Page 60 Operator’s Manual SDF / TDF – Kjeldahl Method To determine the protein content within the SDF or TDF residue, follow the procedure below. Cut the filter bag 8 -10 mm above the filter. Fold the remainder of the filter bag in thirds from side to side.

  • Page 61: Ash Determination

    Operator’s Manual Ash Determination ANKOM Technology recommends that you use ceramic crucibles with fitted covers to avoid cross contamination or loss of ash. Fold the filter bags so that they easily fit into the crucibles. To determine the ash content within the IDF residue, follow the procedure below.
  • Page 62 SDF bag be ashed. To determine the ash content within the SDF / TDF residue, follow the procedure below. Starting at the bottom of the filter part of the bag, fold the bag upward, leaving at least 10 mm at the top of the clear polypropylene that is not folded.

  • Page 63: Productivity Enhancement

    To enhance the productivity of your instrument, you can begin the digestion process (run in the IDF part of the instrument) of a new procedure (IDF/SDF or TDF) while the precipitation process (run in the SDF part of the instrument) of a previous procedure (IDF/SDF or TDF) is still running.
  • Page 64 Prepare samples and configure the instrument for a new digestion. Refer to the IDF/SDF Analysis and TDF Analysis sections of this manual, following all steps except for those that deal with the precipitation process (SDF). This will include interaction with the Touch Screen Display to verify the instrument set-up (as shown in the IDF/SDF Analysis and TDF Analysis sections).
  • Page 65 Operator’s Manual Install new SDF bags on the ANKOM Dietary Fiber Analyzer and continue the run. When an SDF process completes while an IDF process is in progress, the following screen is displayed. The Method & Procedure shown in the top right corner is the one that is running in the top bags.
  • Page 66 You must be careful while in Expert Mode or you could cause problems! The IDF/SDF and TDF analyses both start filling the SDF bag with EtOH about an hour after the IDF process starts. Therefore, if you start the IDF process, and wait more than an hour to properly install your SDF bags, the instrument will deliver EtOH to the empty bottom stations, wasting EtOH, and ruining a run.

  • Page 67: Status Screen

    Box #3 displays the method name along with the most recent procedure that you have started (either “IDF/SDF” or “TDF”). For example, “AOAC 991.43 IDF/SDF” is displayed when the most recent procedure started is an IDF/SDF. If you start a TDF procedure while you are still running the SDF process from an IDF/SDF procedure, “TDF”...
  • Page 68 Bottom process – Name Box #8 always displays “SDF” to represent an SDF process within an IDF/SDF procedure or the precipitation process within a TDF procedure. Bottom process – Progress Box #9 displays the actual time remaining for the bottom process.
  • Page 69 Operator’s Manual When the Display Temperatures button is pressed, the following screen is displayed showing the temperatures of the In- line Heater and all six Heating Paddles. Temperature Monitor (°C) ← Paddle 1 Paddle 2 Paddle 3 Paddle 4 Paddle 5 Paddle 6 In-line Heater The values shown on this screen will dynamically change as the temperatures change.
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  • Page 71: Fault Handling

    Button #2 is only displayed when a low input pressure fault occurs. When button #2 is pressed, the fault is reset. Next fault Multiple faults can occur during a procedure. Pressing button #3 will walk through all of the existing faults. List of Faults and Alerts (see www.ankom.com for the most current Fault Handing information) Name Description Result...
  • Page 72 In-progress processes See "Technical FAQs" Over Temp delivery is reporting a temperature that is are aborted. section at www.ankom.com. higher than the specified range. Paddle Heater One of the Paddle Sensors is reporting a IDF process continues. See "Technical FAQs"...

  • Page 73: Diagnostics Mode

    Operator’s Manual Diagnostics Mode To help solve problems that may occur while running the ANKOM Dietary Fiber Analyzer, and to assist with periodic maintenance procedures, a computer controlled Diagnostics Mode is available through the Touch Screen Display. Select a Function AOAC 991.43...
  • Page 74 Operator’s Manual The value on the Mixer Duty Cycle screen is only used during Diagnostics and in an AOAC 2009.01/2011.25 assay during the 16 hour Amylase/AMG digestion and Trizma phases. Heater Test When you press the Heater Test button on the Diagnostics screen, the following screen will be displayed. Heater Test ←...
  • Page 75 Operator’s Manual Motor Test When you press the Motor Test button on the Diagnostics screen, the following screen will be displayed. Motor Test Vacuum xx.x ← cur: xx.x max: xx.x Motor Speed Amount (ml) 120 RPM 240 RPM Set Valves 10.0 Motor Direction...
  • Page 76 Operator’s Manual Digestion Times When you press the Digestion Times button the following screen will be displayed. AOAC 991.43/985.29/2001.03 ← Digestion Times (Press button with number to change) Amylase Protease HCl/AMG Digestion Time Digestion Time Digestion Time (minutes) (minutes) (minutes) 991.43/985.29 SDF 2001.03 SDF Precipitation Time...
  • Page 77 This diagnostic allows you to pump individual fluids, or all of the fluids, into the lines from the Chemical Containers to the Tubing Support Panel. If by accident you run out of any fluid during a normal IDF/SDF or TDF run, there may not be enough fluid in the lines for the next assay.
  • Page 78 Expert Mode When Expert Mode is “ON,” all of the question screens in the IDF/SDF and TDF procedures are bypassed except for the “Check pH manually?” and “Filter minutes OK?” screens. See the “Productivity Enhancement” section of this manual for more information about Expert Mode.
  • Page 79 Service Mode – AOAC 2009.01/2011.25 Registration When you purchase the upgrade to Total Integrated Dietary Fiber, ANKOM Technology will provide you with a registration number that you will use to configure your instrument to run the AOAC 2009.01 and 2011.25 methods. When you press the AOAC 2009.01/2011.25 Registration button on the Service Mode screen, the following screen will be displayed.
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  • Page 81: Periodic Maintenance

    Replace tubing as needed. Maintain pinch valve tubing On a yearly basis, replace all pinch valve tubing (TDF71) at the IDF, SDF, waste, and enzyme pinch valves. Refer to TDF Service Procedure 5 (TS005) at www.ankom.com. Lubricate peristaltic pump and pump tubing On a monthly basis, apply TDF107 Synthetic Grease to the peristaltic pump tubing and rollers to reduce wear and extend life of the pump tubing.

  • Page 82: Troubleshooting & Replacement Parts

    Operator’s Manual Troubleshooting & Replacement Parts The ANKOM Technology web site has the most current troubleshooting and replacement parts information. Therefore, if you have any questions about the operation of your ANKOM Dietary Fiber Analyzer, or if you need replacement parts, please visit our web site at www.ankom.com.

  • Page 83: Qc / Calibration Checks

    Operator’s Manual QC / Calibration Checks The ANKOM Dietary Fiber Analyzer is an automated and highly integrated instrument. Utilizing an on-board computer, the instrument automatically checks its operation in multiple ways. For example, during each run the instrument automatically checks for Tube Integrity.
  • Page 84 Operator’s Manual Service Mode ← Volume Temperature Calibration Sensor Corrections IDF Count Bypass Amylase Phase Initial AOAC 2009.01/ Heat-up 2011.25 (NO) Registration Press the Volume Calibration button. The following screen will be displayed. Volume Calibration ← Calibration Time START xx.x Label and weigh six small empty glass beakers.
  • Page 85 Operator’s Manual Install extra plastic containers under all six IDF Delivery Nozzles to catch any fluid currently in the lines. With containers in place, press the OK button. After fluid empties into the containers, the following screen will be displayed. Volume Calibration ←...
  • Page 86 Operator’s Manual Volume Calibration ← Final fluid delivery in progress... When the fluid delivery is complete, the following screen will displayed. Volume Calibration ← Enter average volume delivered by pressing the number input, Calibration Time entering the number, then pressing the “SET”...
  • Page 87 Operator’s Manual Status Vacuum xx.x AOAC pH required? cur: - x.x Method max: - x.x Procedure Overall Progress Action in progress Time remaining: IDF Aborted XX:XX:XX Time remaining: XX:XX:XX Main Menu Display Temps/Pressures Press the Main Menu button. The following screen will be displayed. Select a Function AOAC 991.43 AOAC 985.29...
  • Page 88 Operator’s Manual Service Mode ← Volume Temperature Calibration Sensor Corrections IDF Count Bypass Amylase Phase Initial AOAC 2009.01/ Heat-up 2011.25 (NO) Registration Press the Temperature Sensor Corrections button. The following screen will be displayed. Temperature Sensor Corrections ← Enter the difference between actual and Save to measured temperatures.

  • Page 89: Appendix A - Reagents (Aoac 991.43, 985.29, 2001.03)

    Enzyme solutions for AOAC 991.43, 985.29, and 2001.03 methods With no specific enzyme activity standards referred to in the dietary fiber methods, ANKOM has chosen to use the activity numbers specified by Megazyme based on their extensive research. As always, refer to the specific method for Enzyme purity information, and run specified test samples as appropriate.
  • Page 90 (b) Ethanol 95%. (c) Ethanol 78%—Place 821 ml of 95% ethanol into a 1 L volumetric flask. Dilute to volume with H (d) Diatomaceous earth (DE)—(ANKOM DE1, DE2, or equivalent). (e) Acetone—reagent grade. General solutions unique to the AOAC 991.43 method (f) MES—2-(N-Morpholino)ethanesulfonic acid (No.

  • Page 91: Appendix B - Instrument Out-Of-Use Procedure

    BEFORE the instrument is left unused for one week or longer. Flush and purge the lines. These instructions are available with additional pictures in TDF Service Procedure 15 located at www.ankom.com. 1.1 With the instrument turned on, press the Diagnostics button on the Touch Screen Display.
  • Page 92 When returning the instrument to service after a period of non-use, you must close and latch the pump doors and charge all of the lines before running. See the IDF/SDF Analysis, TDF Analysis, or Diagnostics sections of this manual for information about charging the lines.

  • Page 93: Appendix C - De-Fatting Procedure

    1-4 microns. As a result, the likelihood of sample loss is eliminated. To de-fat your sample prior to running the IDF/SDF or TDF analyses, follow the steps below. Number all the XT4 Filter Bags to be used for testing.
  • Page 94 Carefully cut the XT4 Filter Bags inside the IDF Filter Bags or the IDF Flow-Thru Bags on the TDF instrument. To get the XT4 Filter Bag pieces into the TDF bags, hold each XT4 Filter Bag inside the appropriate TDF bag and cut the XT4 into approximately a dozen pieces being careful not to lose any sample.

  • Page 95: Appendix D - Accessories (Sold Separately)

    Operator’s Manual Appendix D – Accessories (sold separately) Contact ANKOM Technology or an authorized dealer to order accessories. TDF51 – Rinse Stand assy DE1/DE2 – Diatomaceous Earth (DE) HS – Heat Sealer (120v) HSi – Heat Sealer (220v) DF-S – SDF Filter Bags (50) DF-I –...
  • Page 96 Automation saves time and money! ANKOM Technology is an international company with products that include… A2000 Fiber Analyzer • Crude Fiber (AOCS Ba 6a-05), ADF, NDF • Automatically adds solutions and rinses • Batch process - up to 24 samples at one time XT15 Fat Extractor •...

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