Ankom Flex Analyte Extractor Operator's Manual page 21

6.9.7.4. Close the evaporation chamber door manually.
6.9.7.5. Press "YES," confirming that all four round bottom flasks are installed properly.
6.9.8. Confirm Solution Reservoirs are Full
6.9.8.1. Confirm that each solution reservoir is filled with the appropriate chemicals. (Refer to Appendix A -
Reagents)
6.9.8.2. Ensure that each reservoir cap is closed tightly.
6.9.8.3. Once all the reservoirs are full, press "YES."
6.9.9. Start Line Prime
When set-up steps have been completed, press "START
RUN."
The Line Prime method will take 7-10 minutes to
complete.
6.9.10. End of Line Prime
6.9.10.1. Turn the large black release ring counterclockwise (to the left), to release the round bottom flasks.
6.9.10.2. Remove the round bottom flasks. They will remain clean during the line prime method and can be used
for the next assay.
6.9.10.3. Remove and clean digestion vessels for subsequent use. Refer to FLEX Service Procedure FLS011.
NOTE:
It is very important to thoroughly clean vessel bottom assemblies after use. Use water, and NOT
ethanol or acetone, as final rinse solution. The digestion vessels must not be left assembled for extended
periods of time (e.g. overnight). If digestion vessels are left assembled for extended periods of time, the O-
rings in the vessel bottom assemblies will deform and future integrity could be compromised.
6.9.10.4. Remove the SPE columns. They will remain clean during the line prime method and can be used for
future assays.
6.9.10.5. Wipe the digestion vessel tops spray
nozzles and O-rings, and the vessel bases
with a towel wetted with water.
6.9.10.6. Dispose of waste bottle reservoir materials according to SDS guidelines and internal SOP. This reservoir
will contain a mixture of all the solutions that has been used in an assay, which may include organic
solvents, strong bases, and acids.
Rev 1/22/2021
Operator's Manual
pg. 23