Qc And Calibrations; Solution Delivery Calibration - Ankom Flex Analyte Extractor Operator's Manual

15. QC and Calibrations

Solution Delivery Calibration

The purpose of the solution delivery calibration is to confirm that the instrument pump is delivering the correct amount of
solution. This calibration should be done when a new chemical is added, or if the user would like to confirm that solution
delivery is accurate. Wear gloves and safety glasses when running this calibration.
15.1.1. Fill each solution reservoir with the appropriate reagents to at least half
the bottle volume, refer to Appendix A - Reagents. Be sure to use the
correct reservoir position for each solution as certain solutions must be
vented (1 and 3) or unvented (2 and 4). Close all reservoir caps when
filled. For further details on how to fill the reservoir bottles, refer to FLEX
Service Procedure FLS005 on the website.
15.1.2. From the Home Screen, press "DIAGNOSTICS."
15.1.3. Select "SOLUTION DELIVERY CALIBRATION."
The following screen will be displayed. Follow
the instructions on the screen.
15.1.4. If calibrating for one new chemical, follow the
calibration steps for that specific reservoir
only. If calibrating all four reservoirs, follow
the specific sequence listed below. Select
reservoir 4 to begin.
Sequence:
1. Reservoir 4 - Hexane
2. Reservoir 1 - EtOH
3. Reservoir 3 - H
4. Reservoir 2 – KOH
5. Reservoir 3 – H
15.1.5. A list of solutions is provided on the right side of the screen. The user will choose the solution that is in the
selected reservoir and then press "Next."
NOTE:
The user can add a new solution by pressing "NEW SOLUTION". The user must enter an accurate
boiling point for safety checks. Once this is complete, press "Next". The instrument cannot run Diethyl Ether
or Acetone.
15.1.6. Label four 100 ml plastic calibration cups, 1-4.
Rev 1/22/2021
O
2
O (Run this reservoir again to clear lines of KOH)
2
Operator's Manual
pg. 45