Metrohm 883 Basic IC plus Manual page 61

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883 Basic IC plus
Connecting the separation column
1 Removing the stoppers
Remove the stoppers from the separation column.
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2 Installing the inlet of the separation column
CAUTION
When inserting the column, ensure that it is inserted correctly
based on the marked flow direction.
There are 3 possibilities:
Attach the column inlet directly onto the guard column or,
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if the guard column is connected to the separation column using
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a connection capillary: Connect the column inlet to the guard col-
umn outlet capillary using a PEEK pressure screw (6.2744.070) or,
if no guard column is used (not recommended): Connect the col-
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umn inlet capillary to the inlet of the separation column using a
short pressure screw (6.2744.070).
3 Rinsing the separation column
Place a beaker under the outlet of the separation column.
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Start manual control in MagIC Net and select the high-pressure
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pump: Manual ▶ Manual control ▶ Pump
– Flow: Increase gradually up to the flow rate recommended
in the column leaflet.
– On
Rinse the separation column with eluent for approx. 10 minutes.
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Stop the high-pressure pump in the manual control in MagIC Net
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again: Off.
4 Removing the coupling
Remove the coupling (6.2744.040) from the column outlet capil-
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lary.
5 Installing the outlet of the separation column
Fasten the column outlet capillary to the column outlet using a
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short PEEK pressure screw (6.2744.070).
3 Installation
53
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