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Metrohm 889 Manual

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889 IC Sample Center
Manual
8.889.8001EN

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  • Page 1 889 IC Sample Center Manual 8.889.8001EN...
  • Page 3 Metrohm AG CH-9101 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com 889 IC Sample Center Manual 8.889.8001EN 02/2010 dm...
  • Page 4 Teachware Metrohm AG CH-9101 Herisau teachware@metrohm.com This documentation is protected by copyright. All rights reserved. Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above.

  • Page 5: Table Of Contents

    Connecting pump and column .......... 15 Tubing ................. 16 3.4.1 Tubing guide ................. 16 Outlet tubings ..............17 Rinsing the syringe ............. 18 4 Functioning Injection modes ..............19 Syringe and buffer loop ............. 20 ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 6 5.3.2 Replacing the sample needle ..........33 5.3.3 Replacing the air needle ............35 5.3.4 Replacing the fuses ..............36 Quality Management and validation with Metrohm ..37 6 Troubleshooting Error list ................38 6.1.1 Rack unit ................38 6.1.2 Needle unit ................
  • Page 7 Declaration of Conformity ..........52 Warranty (guarantee) ............53 Quality Management Principles ........54 10 Accessories 10.1 Scope of delivery 2.889.0010 ..........56 10.2 Scope of delivery 2.889.0020 ..........58 10.3 Optional accessories 2.889.0010 ........59 10.4 Optional accessories 2.889.0020 ........60 Index ￭￭￭￭￭￭￭￭...
  • Page 8 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 889 IC Sample Center - front ............. 7 Figure 2 889 IC Sample Center - rear .............. 8 Figure 3 Interior view without covering ............10 Figure 4 Interior with sampling device ............11 Figure 5 Interior with installed cooling option.

  • Page 9: Introduction

    1 Introduction 1 Introduction Instrument description The 889 IC Sample Center is a robust Autosampler for high sample through- put, optimized for the challenges of the modern analytic laboratory. Its greatest advantages are speed and small sample volumes. Special features: Control of syringes with high resolution.

  • Page 10: Instrument Components

    This instrument is suitable for processing chemicals and flammable samples. The usage of the 889 IC Sample Center therefore requires that the user has basic knowledge and experience in the handling of toxic and caustic sub- stances. Knowledge with respect to the application of the fire prevention measures prescribed for laboratories is also mandatory.

  • Page 11: Safety Instructions

    To maintain this state and ensure non-hazardous operation of the instrument, the following instructions must be observed carefully. 1.3.2 Electrical safety The electrical safety when working with the instrument is ensured as part of the international standard IEC 61010. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 12 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.3 Safety instructions Warning Only personnel qualified by Metrohm are authorized to carry out service work on electronic components. Warning Never open the housing of the instrument. The instrument could be dam- aged by this. There is also a risk of serious injury if live components are touched.

  • Page 13: Tubing And Capillary Connections

    Personnel safety Warning Wear protective goggles and working clothes suitable for laboratory work while operating the 889 IC Sample Center. It is also advisable to wear gloves when caustic liquids are used or in situations where glass vessels could break.

  • Page 14: Flammable Solvents And Chemicals

    The correct disposal of your old equipment will help to prevent negative effects on the environment and public health. More details about the disposal of your old equipment can be obtained from your local authorities, from waste disposal companies or from your local dealer. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 15: Overview Of The Instrument

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument 2 Overview of the instrument Front and rear Figure 1 889 IC Sample Center - front Tubing guide Door to the sample chamber For capillaries and tubings. With handle. Connector for the outlet tubing...

  • Page 16: Opening The Device

    Opening the device Warning Mobile parts are to be found in the interior of the 889 IC Sample Cen- ter. Reaching into the interior during operation puts personnel at a serious risk of injury. Open the door only if the device is in idle mode. Due to safety considerations, no needle movements are carried out by the device when the door is open.
  • Page 17 Opening the door Proceed as follows: 1 Grip the door handle: 2 Carefully pull out the door and press it upwards until it is in a horizontal position. 3 Slide the door into the housing. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 18: Interior View

    1 Press the two black buttons on the sides of the housing (above) simul- taneously. 2 Carefully pull out the covering towards the front. Interior view Figure 3 Interior view without covering ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 19: Interior

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument Interior The following parts are to be found in the interior of the 889 IC Sample Center: Figure 4 Interior with sampling device Syringe Needle arm Injection valve Sample drip pan Sample chamber Needle washing position Washing bottle ￭￭￭￭￭￭￭￭...

  • Page 20: Cooling Option

    Figure 5 Interior with installed cooling option. If the cooling option is installed, pull the covering of the cooling option off towards the front. Now you can place the sample racks or micro titer plates. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 21: Installation

    Grip the device underneath with both hands when you are transporting it. Always hold the device in an upright position. Before you switch on the 889 IC Sample Center, allow the device to stand for at least one hour in order to adapt to the room temperature.

  • Page 22: Connecting A Computer

    Objects placed on the device could also impair the cooling output. Objects can be placed at the sides of the 889 IC Sample Center. If an object is placed on only one side, then a minimum clearance of 5 cm must be maintained.

  • Page 23: Connecting Pump And Column

    MagIC Net™ . Connecting pump and column The 889 IC Sample Center is supplied with the necessary mounted hose and capillary connections. Connect the high pressure pump and the separation columns to the still unoccupied ports 1 and 6 on the injection valve with one suitable PEEK capillary each (6.1831.010).

  • Page 24: Tubing

    Port 6 of the injection valve. Sample loop Port 2 and 5 of the injection valve. Tubing The 889 IC Sample Center is equipped with the following standard tubing: Tubing/Capillary Material/Dimensions Standard sample needle and SS (inert-coated): 97 mm x 0.8 mm OD capillary (15 µL)

  • Page 25: Outlet Tubings

    General waste Connect one outlet tubing (included in the scope of delivery) on the left- hand tubing connector on the front of the 889 IC Sample Center. Guide the other end into a waste container underneath the 889 IC Sample Center.

  • Page 26: Rinsing The Syringe

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.6 Rinsing the syringe Rinsing the syringe The syringe must be rinsed bubble-free prior to the start-up of the 889 IC Sample Center. Fill the washing bottle with isopropanol. Warning Do not use any saline or buffer solutions. Crystals could block or damage the entire system.

  • Page 27: Functioning

    The syringe aspirates the sample out of the sample vessel and into the sample loop. The buffer loop between the syringe and the injection valve prevents the contamination of the syringe. Washing solution is required in order to: ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 28: Syringe And Buffer Loop

    The pickup injection offers maximum accuracy (the same as with partial loopfill) and no loss of sample, but somewhat lower reproducibility, namely a relative standard deviation (RSD) better than 1% for injection volumes > 10 µL. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 29: Full Loop Injection

    The injection valve is in INJECT position. The sample needle penetrates the vial with the air needle. An overpressure channeled in through the air needle ensures that no air or steam bubbles form during the aspi- ration of the sample. 2 Rinsing the aspirating line ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 30 4 Filling the sample loop The sample loop is quantitatively filled by a multiple of the loop volume being pumped through the loop. 3 × loop volume with sample loops ≤ 100 µL ￭ ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 31 30 µL for one injection, and at least 35 µL for injections without air segments. In the case of high-viscosity samples, larger rinsing volumes must be programmed and the speed of the syringe stroke must be reduced in order to improve reproducibility. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 32: Partial Loopfill Injection

    The injection valve is in INJECT position. The sample needle penetrates the vial with the air needle. An overpressure channeled in through the air needle ensures that no air or steam bubbles form during the aspi- ration of the sample. 2 Rinsing the aspirating line ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 33 The injection valve is switched over to the LOAD position. This causes an homogenous sample plug to be present at the inlet to the sample loop. 4 Filling the sample loop partially The programmed injection volume is now aspirated into the sample loop. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 34 30 µL for one injection, and at least 35 µL for injections without air segments. In the case of high-viscosity samples, larger rinsing volumes must be programmed and the rate of the syringe stroke must be reduced in order to improve reproducibility. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 35: Pickup Injection

    3 Filling the aspirating line with transport solution For the first injection, the syringe aspirates a segment of transport sol- ution in order to fill the sample line with transport solution. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 36 4 Switching the injection valve to LOAD The needles switches from the washing position to the sample vial. The injection valve switches to the LOAD position. 5 Aspirating sample The programmed injection volume is aspirated from the sample vial. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 37 The injection valve switches to the INJECT position. The sample loop is now part of the flow path of the eluent; the sample is transported to the separation column. The determination is started. This sequence is repeated for each injection. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 38 No overpressure is permitted to be applied in the sample vessel, because the air segment would expand during the change from sample position to transport position. This could lead to a considerable error with respect to the injection volume of the sample. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 39: Handling And Maintenance

    4 Remove the covering by pulling towards the front. 5.2.1 Cleaning in general Generally speaking, the 889 IC Sample Center requires little upkeep. Clean the outside of the housing with a soft cloth and a mild cleaning fluid. Other parts which require regular care include: Valve drip pan.

  • Page 40: Maintenance And Service

    5.3.1 Sample loop The 889 IC Sample Center is equipped as standard with a 100 µL sample loop. Other sample loops can be mounted. Please note however that the correct combination of syringe and buffer loop is required in order to achieve good results.

  • Page 41: Replacing The Sample Needle

    Replacing the sample needle Replace the sample needle as follows: 1 Open the Manual control in MagIC Net™ . 2 Under device selection, 889 IC Sample Center, select the tab Nee- dle. Under Position/Input, select Exchange position and click on [Start].
  • Page 42 5.3 Maintenance and service The needle moves into the exchange position. 4 Open the door of the 889 IC Sample Center. 5 If working with the cooling option, remove the covering of the cooling option by pulling it towards the front.

  • Page 43: Replacing The Air Needle

    Wash function. To interrupt the rinsing procedure, click on Stop. Note The needle settings may need to be adjusted. In MagIC Net™, open the configuration settings of the 889 IC Sample Center and select the Needle tab. Perform the necessary setting.

  • Page 44: Replacing The Fuses

    General tab, under Wash, click on Start. To interrupt the rinsing pro- cedure, click on Stop. 5.3.4 Replacing the fuses The fuses installed in the 889 IC Sample Center are of the type 2 × 2.5 A Warning The device must be disconnected from the electricity source before the fuses are replaced.

  • Page 45: Quality Management And Validation With Metrohm

    Metrohm agent. Validation Please contact your local Metrohm agent for support in validating instru- ments and software. Here you can also obtain validation documentation to provide help for carrying out the Installation Qualification (IQ) and the Operational Qualification (OQ).

  • Page 46: Troubleshooting

    6.1 Error list 6 Troubleshooting The 889 IC Sample Center is controlled by the MagIC Net™ PC software. If a problem occurs which is directly related to the 889 IC Sample Center, then an error number will be identified in a message window. The significance of the error numbers is listed in the following chapter.

  • Page 47: Needle Unit

    In order to reach the horizontal 1. Check whether the horizontal move- Home position of the needle, ment of the needle is obstructed. the expected number of steps 2. If possible, remove possible obstacles. has not been carried out. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 48 2. If possible, remove possible obstacles. If the error occurs again, please call the Metrohm Service. Error 319 The sample needle arm is loca- Call the Metrohm Service. ted in an undefined vertical position. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 49: Syringe Unit

    Ensure that the required unload vol- ￭ ume of the syringe is lower or the same as the maximum syringe vol- ume. Check whether the required unload ￭ volume is possible assuming the cur- rent position of the syringe piston. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 50: Injection Valve Unit

    Have a look at the technical specifications a temperature of over 48 °C in the device manual of the sample has been measured. changer and ensure that the range of the operating temperature has not been exceeded. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 51: Electronics

    Contact your service point for additional help. 6.2.1 Autosampler Problem Cause Remedy The reproducibility There is air in the flow path. Carry out a standard washing procedure does not correspond to (in MagIC Net™ under Manual con- the specifications. trol). ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 52 2. Carry out a standard washing proce- dure. 3. If washing solution is flowing out of the injection port, check the needle. If no washing solution is flowing out of the injection port, remove the buffer tubing from the valve. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...
  • Page 53 6. If the ports 3 and 4 are leaking, check the rotor sealing. If not, manually test the valve again. Warning Observe the maximum pressure of 350 bar in order to avoid any valve leakage. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 54: Appendix

    I/O interface Caution The manufacturer assumes no liability for damage which is either directly or indirectly caused by connections between the 889 IC Sample Center and devices which do not meet the relevant safety standards. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 55: Properties Of The I/O Interface

    Pin assignment of I/O socket and plug Table 1 Inputs and outputs of the I/O interface Pin No. Assigment Output - Common (COM) Output - Normally open (NO) Input 1 (TTL) Input 2 (TTL) Output - Normally close (NC) +5 volts ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 56: Technical Specifications

    Integrated container for the washing solution Moistened materi- PTFE, TEFZEL, PEEK als in the flow path Injection cycle time < 60 s, in all injection modes for 1 injection≤ 100 µL including 300 µL wash cycle ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 57: Analytical Characteristics

    Range: 4 °C to 3 °C below ambient temperature Air temperature in the sample vessel: 4 °C ±2 °C (at the temperature sensor) (Temperature at 80% relative humidity and ambient temperature of 25°C) ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 58: Mains Connection

    EN/IEC 61000-3-2 ￭ EN/IEC 61000-3-3 ￭ Immunity Standards fulfilled: EN/IEC 61326-1 ￭ EN/IEC 61000-6-2 ￭ EN/IEC 61000-4-2 ￭ EN/IEC 61000-4-3 ￭ EN/IEC 61000-4-4 ￭ EN/IEC 61000-4-5 ￭ EN/IEC 61000-4-6 ￭ EN/IEC 61000-4-8 ￭ EN/IEC 61000-4-11 ￭ ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 59: Ambient Temperature

    360 mm Depth 1.889.0010 510 mm 1.889.0020 575 mm Weight 1.889.0010 19 kg 1.889.0020 21 kg Material of hous- Cover Steel Base Steel Side panels PC/ABS (UL 94 V0) Front PC/ABS (UL 94 V0) PMMA Panes ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 60: Conformity And Warranty

    Name of commodity 889 IC Sample Center Autosampler for usage in ion chromatography systems. This instrument has been built and has undergone final type testing accord-...

  • Page 61: Warranty (Guarantee)

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9 Conformity and warranty Metrohm Ltd. is holder of the SQS certificate ISO 9001:2000 Quality man- agement system for development, production and sales of instruments and accessories for ion analysis. Herisau, 10 Mai, 2009 D. Strohm A. Dellenbach Vice President, Head of R&D...

  • Page 62: Quality Management Principles

    Components All components used in the Metrohm instruments have to satisfy the quality standards that are defined and implemented for our products. Suppliers of components are audited by Metrohm as the need arises.
  • Page 63 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9 Conformity and warranty cation and Performance Verification of the system components or in carrying out the System Validation for the quantitative determination of a substance in a given matrix. ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 64: 10 Accessories

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 10.1 Scope of delivery 2.889.0010 10 Accessories Note Subject to change without notice. 10.1 Scope of delivery 2.889.0010 Qty. Order no. Description 1.889.0010 IC Sample Center 6.1608.110 Wash bottle 250 mL to IC Sample Center Bottle for washing solution in the IC Sample Center.
  • Page 65 6.2743.1xx 6.2151.020 Cable USB A - USB B 1.8 m USB connecting cable Length (m): 6.2743.107 PP Vials 0.7 mL to 889, 100 pcs 100 sample vials. 6.2743.127 Septum to 6.2743.1XX Si/PTFE 100 pcs 100 pieces 6.2744.010 Pressure screw 5x With UNF 10/32 connection.

  • Page 66: Scope Of Delivery 2.889.0020

    Sample rack for the IC Sample Center with 48 sample positions. For sample vials 6.2743.1xx 6.2151.020 Cable USB A - USB B 1.8 m USB connecting cable Length (m): 6.2743.107 PP Vials 0.7 mL to 889, 100 pcs 6.2743.127 Septum to 6.2743.1XX Si/PTFE 100 pcs ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

  • Page 67: Optional Accessories 2.889.0010

    6.2122.020 Germany, …: Type CEE(7), VII 6.2122.040 USA, …: Type NEMA/ASA 6.2122.070 U.600.0020 Microfuse 8.889.8001EN Manual 889 IC Sample Center 10.3 Optional accessories 2.889.0010 Qty. Order no. Description 6.1562.170 Tefzel transfer tubing 1000 µL To IC Sample Center 6.1829.060 PTFE capillary with connector / 1/8 in. / 1/16 To IC Sample Center 6.2743.100...

  • Page 68: Optional Accessories 2.889.0020

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 10.4 Optional accessories 2.889.0020 Qty. Order no. Description 6.2743.117 PP Vials 0.3 mL to IC Sample Center 100 sample vials. 6.2743.120 Septum to 6.2743.1XX Si/PTFE 1000 pieces 6.2816.210 Needle with capillary, 15 µL To IC Sample Center 10.4 Optional accessories 2.889.0020 Qty.

  • Page 69: Index

    Validation ......... 37 (EMC) ........50 Vial .......... 46 Electrostatic charge ....4 Outlet tubings ......17 Warranty ........53 Full loop Injection ..... 21 Partial loopfill injection ..... 24 Windows ........15 Fuse ......... 36 ￭￭￭￭￭￭￭￭ 889 IC Sample Center...

This manual is also suitable for:

2.889.0010 8892.889.0020 889

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