Loading The Rotor; Closing Biosafety Systems - Hettich ROTOFIX 46 Operating Instructions Manual

11

Loading the rotor

Standard centrifuge containers of glass will not stand RCF values exceeding 4000 (DIN 58970, pg. 2).

Check the rotor for firm seating.

With swing-out rotors all rotor positions must be lined with identical hangers. Certain hangers are marked with
the number of the rotor position. These hangers may only be used in the respective rotor position. Hangers that
are marked with a set number (e.g. S001/4) may only be used in the set.

The rotors and hangers may only be loaded symmetrically. The centrifuge containers have to be distributed
evenly on all rotor positions. For authorised combinations see Chapter "Anhang/Appendix, Rotoren und
Zubehör/Rotors and accessories".
In the case of angle rotors all possible rotor positions must be loaded, see chapter "Anhang/Appendix, Rotoren
und Zubehör/Rotors and accessories".
Rotor is evenly loaded

On certain hangers, the weight of the maximum load or the weight of the maximum load and the maximum
weight of the completely loaded hanger is specified. This weight may not be exceeded. In case of exception, see
chapter " Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm
specified for the maximum loading includes the total weight of adapter, frame, centrifuging container and content.

In containers with rubber inserts, the same number of rubber inserts must always be among the centrifuge
containers.

Always fill the centrifuge containers outside of the centrifuge.

No liquid should be allowed to enter the centrifugal chamber during filling and swinging out of the hangers.

The maximum filling quantity for the centrifuge containers specified by the manufacturer must not be exceeded.

In order to maintain the weight differences within the centrifuge container as marginal as possible, a consistent
fill level in the containers is to be heeded.
12

Closing biosafety systems

In order to guarantee that it is sealed, the lid to the biosafety system has to be firmly closed.
In order to prevent the packing ring from twisting when opening and closing the cover, the packing ring must
be lightly rubbed with talcum powder or a rubber care product.
If the hanger of a biosafety system is used without the lid, the packing ring must be removed from the hanger
in order to prevent the packing ring from being damaged during the centrifugation run. Damaged packing rings
must not be used to seal the biosafety system.
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
Lid with screw top:

Close the lid firmly by hand by turning in clockwise direction.
Not permitted!
Rotor is not evenly loaded
EN
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".The weight
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