9
•
Specifications
Time constant and response time
9.1.2
Flow Cells
Type
Standard flow cell, SST
Standard Bio flow cell,
PEEK
Semi-micro flow cell, SST
Semi-micro Bio flow cell,
PEEK
Semi preparative flow cell
Nano flow cell
Capillary flow cell
Mic flow cell
Page 152
For best performance, minimize the frequency and the amplitude of
changes in the ambient temperature to 1 °C/hour.
According to ASTM E1657-98, the time constant is converted to the
response time by multiplying by the factor 2.2 (i.e., response time =
2.2 x time constant).
The flow cell specifications are as follows:
Path
Illuminate
length
d volume
10 mm
11 µL
10 mm
11 µL
7 mm
2.5 µL
7 mm
2.5 µL
0.4 mm
0.7 μL
10 mm*
3 nL
10 mm*
45 nL
10 mm*
180 nL
* There is no exact definition of the light path in capillary flow cells as is for conventional
flow cells. Therefore, you should not use this value for calculating the absorption
coefficient or the detector sensitivity according to the Lambert-Beer law.
Pressure
Materials in the
limit
flow path
12 MPa
Stainless steel,
fused silica, PEEK,
fluoropolymers,
titanium
5 MPa
PEEK, Fused silica
12 MPa
Stainless steel,
fused silica, PEEK,
fluoropolymers,
titanium
5 MPa
PEEK, fused silica
10 MPa
PEEK, fused silica
20 MPa
Fused silica
20 MPa
Fused silica
20 MPa
Fused silica
Variable Wavelength Detectors (VC-D40, VF-D40)
Biocompatibility
No
Yes
No
Yes
Yes
Yes
Yes
Yes
Operating Manual