Thermo Scientific Vanquish VC-D40 Operating Manual page 96

6 • Operation
Page 96
Situation after Shutdown
Device and flow cell shall
be transported or shipped
after shutdown
NOTICE
Residual samples, impurities from the column or buffers with high salt
concentrations can deposit in the flow cell. This can lead to damage of
the flow cell. In addition, solvents containing acid can damage the flow
cell.
• Always flush the flow cell with an appropriate solvent before
interrupting operation.
• Fill the flow cell with pure isopropanol using the flushing and
injection kit.
• The flow cell should not be filled with pure water to avoid the
growth of algae. If you want to fill the flow cell with water, you need
to add 10% HPLC-grade isopropanol.
6. Turn off the pump flow to the device. Wait until the system pressure
is down to zero before you continue the shutdown of the device.
7. Disconnect the capillaries from the flow cell inlet and outlet.
8. After removing the flow connections, protect the flow cell inlet and
outlet ports with the plugs supplied with the flow cell. Protect the
inlet capillaries with caps.
9. The step depends as follows:
Situation
Device and all other system modules
remain in the system stack and are to be
turned off
If no additive is used
Flush the system with
isopropanol.
Steps
Turn off the system with the system
power button on the system base.
Variable Wavelength Detectors (VC-D40, VF-D40)
If an additive is used
Flush the system first with
several volumes of
methanol and water
(50:50) (for example,
1.0 mL/min for
10 minutes with the
standard system) to
prevent salt buildup in the
fluidics. If the solvents in
the flow cell are not
miscible with water, use
an appropriate
intermediate solvent.
Afterward, flush the
system with isopropanol.
Operating Manual