Thermo Scientific Vanquish VC-D40 Operating Manual page 83

System Equilibration
Variable Wavelength Detectors (VC-D40, VF-D40)
Operating Manual
System equilibration should include the following operations:
• Purging the pump (all channels, including those not used for the
application)
• Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run
• Warming up (or cooling down) all temperature-controlled devices in
the system to the starting temperature. Temperature-controlled
devices can be, for example
Column compartment and post-column cooler
¨
Sample compartment thermostatting in the autosampler
¨
Flow cell in a fluorescence detector
¨
Evaporation tube in a charged aerosol detector
¨
• Turning on the lamp (or lamps) in the UV/VIS detector
• Monitoring the pump pressure and pressure ripple and checking
that the pressure is stable and the ripple within reasonable limits for
the application
• Monitoring the detector signal and checking whether the detector
signal is stable so that the drift and signal noise are within
reasonable limits for the application
• Performing an autozero of the detector baseline
TIP The Chromeleon software supports procedures for automatically
starting a chromatography system in the software (Smart Startup). The
startup procedure includes the operations for system equilibration. For
details, refer to the Chromeleon Help.
TIP When you start the data acquisition or perform an autozero, the
shutter in the light path before the flow cell is opened automatically.
After the data acquisition, close the shutter, if required.
6 • Operation
Page 83