Reflected Light Brightfield/Darkfield Observation Procedure - Olympus BX-RLA2 Instructions Manual

Reflected light brightfield/darkfield illuminator
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REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE

}The following flow shows the basic operating procedure for reflected light brightfield or darkfield observation. The operating
procedures for polarized light and Nomarski DIC observations will be described separately in their descriptions. (The micro-
scope frame used is the BX51TRF.)
Select the brightfield (BF) or
darkfield (DF) observation.
Set the main switch to " I "
(ON).
Disengage the
analyzer,
polarizer, filter,
etc. from the
light path.
Check
interlocking of
the ND filter.
Select the light path
(trinocular tube only).
Place the specimen on the
stage.
Engage the 10X objective in
the light path.
5
(Controls Used)
(Page)
@ Mirror selector lever
(P. 7)
² Main switch
³ ND filter knob
(P. 10)
| Light path selector knob
ƒ Stage plate
... X/Y-axis knobs
† Revolving nosepiece
Bring the specimen in focus.
Adjust the
brightness.
Adjust the
interpupillary
distance.
Adjust the
diopter.
Adjust the aperture iris dia-
phragm and field iris dia-
phragm.
} Open both iris diaphragms
in case of DF observation.
Engage the desired objective
in the light path and bring the
specimen in focus.
Insert the
required
filters.
Adjust the
brightness.
Start observation.
(Controls Used)
(Page)
‡ Coarse/fine adjustment
knobs
Š Brightness control knob
‰ Binocular tube
‹ Diopter adjustment ring
ΠAS knob
(P. 9)
™ FS knob
(P. 7/8)
† Revolving nosepiece
‡ Coarse/fine adjustment
knobs
š Filter insertion slot
(P. 11)
Š Brightness control knob

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