Transmitted Light Darkfield Observation; Observation Procedure - Olympus BX45 Instructions Manual

4-2 Transmitted Light Darkfield Observation

1
Mounting the Optical Device (DF Ring)
}The optical device (DF ring) can be mounted in the same way as the optical device (PH ring). Please see page 18.
2

Observation Procedure

# Disengage the polarizer and analyzer from the light path.
1. Turn the turret (@ in Fig. 33) to engage the BX45-DFA in the light path.
2. Engage an applicable objective in the light path.
UIS2 Series
DF Ring
BX45-DFA
UIS Series
DF Ring
BX45-DFA
* Objectives with magnification of 10X or more and NA or 0.7 or less can be used.
Objectives equipped with iris diaphragm can also be used if their NA can be reduced to no more than 0.7.
3. Place the specimen on the stage and focus on the specimen.
4. Remove the eyepieces, look into the eyepiece sleeves to locate the objective pupil, and turn the optical device centering
screw holes (above the
5. Attach the eyepiece again, observe the darkfield image, and repeat centering to obtain the best possible darkfield effects.
6. Adjust the condenser height to obtain uniform darkfield illumination across the upper and lower halves of the condenser
image.
7. Open the field iris so that regular illumination is obtained.
When switching the darkfield observation objective or the observation mode between darkfield and another
method, be sure to keep your eyes away from the eyepiece.
When you switch the objective or turret between the darkfield and other positions while looking into the eyepiece.
Otherwise, the direct light may enter your eyes.
20
PlanCN10X, PlanCN20X, PlanCN40X, PlanN10X, PlanN20X, PlanN40X, PlanN50XOI,
UPlanFLN10X, UPlanFLN20X, UPlanFLN60XOI, UPlanFLN100XOI, UPlanSApo10X
Ach10X, Ach20X, Ach40X, Plan10X, Plan20X, Plan40X, Plan50XOI, UPlanFl10X, UPlanFI20X,
UPlanFI100XOI3, UPlanApo10X, UPlanApo20X, UPlanApo100XOI3
markings) to center the DF ring so that no light exits through the objective pupil.
Applicable Objectives*
Applicable Objectives*