Running Protocols; Prepare The Instrument For Running A Protocol; Prepare The Reagents And Plasticware - Agilent Technologies Magnis NGS Prep System User Manual

Running Protocols

Prepare the instrument for running a protocol

Prepare the Magnis/Magnis Dx NGS Prep System so that it is ready to run a protocol.
1 Verify that the instrument deck is cleared of all labware from previous runs.
2 Turn on the instrument and close the instrument door.
See
3 At the
Each time the system is turned on, it performs a series of start up activities (see
page 21) immediately after login. These activities may take several minutes to perform. Make
sure the instrument door remains closed for the duration of these activities. After completion
of the start up activities, the software opens to the
blue, indicating that it is ready for use.
4 (Optional) Perform the 30-minute UV decontamination quick cycle to decontaminate the
surfaces of the instrument deck. See
You can begin
For instructions on manually decontaminating the surfaces of the instrument deck, see
the deck surfaces and instrument exterior"

Prepare the reagents and plasticware

Prepare the samples, target enrichment reagents, and other materials needed for the protocol run
according to the user manual for your specific Magnis Target Enrichment Kit. The user manual
contains details on the materials required to run a protocol and instructions on loading your DNA
samples into the Magnis Sample Input Strip.
The image below describes the orientation of the Magnis Sample Input Strips. Make sure to track
the locations of your samples as you load them into the strip.
Figure 18 Orientation of the Magnis Sample Input Strips
CAU T I ON
Magnis and Magnis Dx NGS Prep System User Guide
"Turn on the instrument"
Login screen, enter the credentials for your user account.

"Prepare the reagents and plasticware"

Do not add any writing or labels to the strips, plates, and other labware that
may obscure the barcodes.
on page 20 for instructions.
Home screen
"Decontaminating with UV Light"
on page 51.
and the system's LED light is
on page 41.
while the decontamination is running.
Figure 6 on
"Clean
35