Agilent Technologies MX3000P Installation Instructions

M 3000P/M
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NSTRUMENT
Plate Storage and Handling Instructions
The plate is shipped on dry ice. Store at –20°C upon receipt. Do not remove the foil wrapper until immediately
before performing the run.
Wear chemical-resistant powder-free gloves whenever handling the qualification plate. This will ensure that no
finger oils or other debris are transferred to the optical seal.
Preparing for the Qualification Test
Note
The Mx3000P or Mx3005P instrument and MxPro software must be set up, according to the
instructions in the system Setup and User's Guide, prior to running the Qualification Test.
The the Qualification Test requires filter gain settings of ×8 for FAM and ×1 for ROX as per factory
settings. To check and modify the filter gain settings, click
Just before starting the Instrument Qualification Test run, thaw the plate at room temperature for
15–30 minutes, leaving the plate sealed in the foil wrapper while thawing.
After thawing, remove the foil wrapper and then mix the contents of the wells by completing the following
actions five times:
• Invert the plate. Use a sharp, swift, downward motion to move the liquid to the seal on top of each well.
Observe the liquid to verify that the liquid has moved from the bottoms to the tops of the tubes.
• Re-invert the plate, and use the same motion to return the liquid to the bottom of each well, again verifying
liquid movement before proceeding.
After completing five mixing cycles, collect the liquid at the bottom of the wells. Brief centrifugation of the
plate at 1000 rpm is highly recommended, in order to dislodge any bubbles and to collect the liquid.
If a plate centrifuge is not available, shake the liquid down to the bottom of the tubes (using the same motion
described above for mixing). Shake-down the plate until the majority of the liquid is at the bottom of the tube
and only minimal amounts of liquid are retained on the tube seal or sides. It is important to shake-down any
noticeable liquid on the tube seal (e.g. visible semi-circles or crescent shapes). Small amounts of liquid spray
retained on the tube sides should not affect the test results.
Prior to loading the plate, verify that no bubbles are present at the bottoms of the tubes, beneath the liquid.
While it is critical to remove any bubbles from the bottom of the tubes, small bubbles near the liquid meniscus
should not affect the test results.
Important
Bubbles trapped at the bottom of the tubes will interfere with fluorescence readings, invalidating
the test.
3005P QPCR S
UALIFICATION
YSTEM
β–A
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CTIN NSTALL
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LATE NSTRUCTIONS
Instrument > Filter Set Gain Settings.