Istance Calculation Module; Objectives And Output - UVITEC Cambridge FIREREADER MAX User & Service Manual

Objectives and output

The FireReader 1D Distance module features the calculation of electrophoretic distances
according to markers or standards:
- in R.F. values
- in isoelectric points
This function allows the assignment of RF values to the detected bands. To do so, an origin
line and a front line must be defined. Usually the origin is set to value 0 and the front to 1,
but the FireReader 1D allows you to set your own values for origin and front lines.
At the end of the process, you can have the following outputs:
- Profile and migration curve
- Calculated distance in between a front line and an end line.
The distance calculation process follows the band detection:
Note: you can access the distance menu by clicking directly on the distance tab.
UVITEC – Cambridge
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FIREREADER FIREREADER
FIREREADER FIREREADER
D
I S T A N C E C A L C U L A T I O N M O D U L E
SERIES SERIES
SERIES SERIES
(values between 0 and 1)
(pH units)