UVITEC Cambridge FIREREADER MAX User & Service Manual page 6

EXPOSURE MENU ......................................................................................................................................................44
Access to the Exposure modules ............................................................................................................................... 44
Auto-exposure ........................................................................................................................................................... 46
Manual exposure ...................................................................................................................................................... 49
Serial exposure .......................................................................................................................................................... 52
Focus point adjustment and motorized zoom controls ............................................................................................. 54
ANALYSIS MENU ........................................................................................................................................................56
Access to the analysis modules ................................................................................................................................. 56
M
OLECULAR WEIGHT ANALYSIS MODULE
Objectives and output ......................................................................................................................................................58
Molecular weight module (MW) operating environment ........................................................................................59
Toolbar in details..................................................................................................................................................................62
1 - D ............................................................................................................................................................. 69
E T E C T
A - Edit lane ..........................................................................................................................................................................69
B - Edit band .........................................................................................................................................................................73
C - Add marker values ......................................................................................................................................................77
2 - A - M
N A L Y S E
A - Molecular weight results .............................................................................................................................................82
Return to the 1D acquisition module ........................................................................................................................ 83
C
OLONY COUNTING ANALYSIS MODULE
Objectives and output ......................................................................................................................................................84
Colony counting (CC) module operating environment ..........................................................................................85
Toolbar in details..................................................................................................................................................................87
A
U T O M A T I C C O U N T I N G
Detection ..............................................................................................................................................................................94
Return to the 1D acquisition module ........................................................................................................................ 95
M
A N U A L C O U N T I N G
Manual counting .................................................................................................................................................................96
Return to the 1D acquisition module ........................................................................................................................ 97
D
ISTANCE CALCULATION MODULE
Objectives and output ......................................................................................................................................................98
Distance calculation module operating environment .............................................................................................99
Toolbar in details................................................................................................................................................................101
1- D ......................................................................................................................................................... 108
E T E C T
A - Edit lane ........................................................................................................................................................................108
B - Edit band .......................................................................................................................................................................111
C - Distance .......................................................................................................................................................................114
2 - A - D
N A L Y S E
A - Distance results ...........................................................................................................................................................117
Return to the 1D acquisition module ...................................................................................................................... 118
O 1D
PTICAL DENSITY ANALYSIS MODULE
Objectives and output ....................................................................................................................................................119
Optical density / 1D (OD-1D) operating environment ............................................................................................119
Toolbar in details................................................................................................................................................................122
1- W
I N D O W D E F I N I T I O N
A - Lane definition ............................................................................................................................................................128
B - Background subtraction ...........................................................................................................................................131
C -Spot separation ...........................................................................................................................................................136
2- A - S
N A L Y S E
Principles of quantification .............................................................................................................................................140
A -Quantity of reference ................................................................................................................................................141
Return to the 1D acquisition module ...........................................................................................................................144
EDIT MENU .............................................................................................................................................................. 145
Access to the Edit module ....................................................................................................................................... 145
Text ......................................................................................................................................................................... 146
Crop ......................................................................................................................................................................... 150
Rotation .................................................................................................................................................................. 151
Pseudo color ............................................................................................................................................................ 152
UVITEC - Cambridge
..........................................................................................................................58
O L E C U L A R W E I G H T
............................................................................................................................84
.................................................................................................................................... 94
........................................................................................................................................... 96
...................................................................................................................................98
............................................................................................................................... 117
I S T A N C E
........................................................................................................................ 119
.............................................................................................................................. 128
P O T Q U A N T I F I C A T I O N
........................................................................................................... 82
................................................................................................... 140
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