C -Spot Separation - UVITEC Cambridge FIREREADER MAX User & Service Manual

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Click on the "Help" button. You automatically access the user manual at the chapter
corresponding to the function
C –Spot separation
In order to measure the volume of a particular spot, you need:
To define the boundary around the spot;
To compare the intensity data inside the boundary with the data of other spots or of a
standard.
A volume is the sum of the pixel intensity inside a defined boundary. The purpose of the spot
separation is to define this boundary.
The spot separation process follows the background subtraction.
Note: you can either access the spot separation function by clicking on the next button of the
background subtraction or directly by clicking on the spot separation of the Window definition
folder.
The dashboard details the spot separation parameters:
Modify the spot separation
Define a threshold
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