UVITEC Cambridge FIREREADER MAX User & Service Manual page 91

UVITEC – Cambridge
91/225
Before
A saturated image is inappropriate for image quantification
with image analysis software. The saturation option allows you to
visualise in red, pixels that have the maximum grey level (4095 for a 12-
bit image or 16 383 for a 14-bit image or 65536 for a 16-bit image) in
order to avoid to flatten the peaks.
Select the saturation option and the saturated pixels are displayed in
red:
Note: A saturated image creates quantification error when studied by
image analysis software. Gel-doc systems have to indicate to the user if
the image is saturated and if it is then necessary to modify the
integration time.
Saturated pixels are not
displayed
The pseudo colors can display different types or levels of fluorescence in
an image. It replaces the original grey levels of the image by another
palette color. The 1D software has several predefined palette designs.
Select your palette design from the followings:
Original image
After
Saturated pixels are
displayed in red
Coomassie painted image